Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products

Autores
Astoreca, Andrea Luciana; Ortega, Leonel Maximiliano; Fígoli, Cecilia Beatriz; Cardós, M.; Cavaglieri, L.; Bosch, María Alejandra; Alconada Magliano, Teresa María
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The concern regarding toxicity from the presence of deoxynivalenol (DON) in wheat that affects both economy and public health leads to the need to find appropriate detection methods for determining the degree of DON contamination in terms of the equipment available and the speed required for obtaining the incidence. The objective of this study was to compare the performance of two alternative analytical techniques for DON quantification for use in the food industry with a reference technique. Samples of wheat and the commercial by-products were analysed by high-performance liquid chromatography (HPLC) with an ultraviolet detector as the reference method and the results compared with those obtained from a rapid lateral-flow immunochromatographic device (Reveal Q+) and of a Fourier-transform-infrared (FTIR) spectroscopy technique. Pearson’s correlation coefficient between the HPLC and Reveal-Q+ data (0.45), although significant (P<0.0003), was lower than that obtained between HPLC and the FTIR method (0.94, P<0.0001). Both methods were considered efficient in quantifying DON levels in wheat-flour samples. This study was aimed at assisting the producers in choosing an appropriate tool for the purpose of analysis and upon consideration of the available equipment.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI)
Materia
Química
deoxynivalenol
wheat
high-performance liquid chromatography
Fourier-transform infrared spectroscopy
lateral-flow immunochromatography
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/79428
Acceder
id SEDICI_45e947c416d5f96c4707fd52ef3c91fd
oai_identifier_str oai:sedici.unlp.edu.ar:10915/79428
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial productsAstoreca, Andrea LucianaOrtega, Leonel MaximilianoFígoli, Cecilia BeatrizCardós, M.Cavaglieri, L.Bosch, María AlejandraAlconada Magliano, Teresa MaríaQuímicadeoxynivalenolwheathigh-performance liquid chromatographyFourier-transform infrared spectroscopylateral-flow immunochromatographyThe concern regarding toxicity from the presence of deoxynivalenol (DON) in wheat that affects both economy and public health leads to the need to find appropriate detection methods for determining the degree of DON contamination in terms of the equipment available and the speed required for obtaining the incidence. The objective of this study was to compare the performance of two alternative analytical techniques for DON quantification for use in the food industry with a reference technique. Samples of wheat and the commercial by-products were analysed by high-performance liquid chromatography (HPLC) with an ultraviolet detector as the reference method and the results compared with those obtained from a rapid lateral-flow immunochromatographic device (Reveal Q+) and of a Fourier-transform-infrared (FTIR) spectroscopy technique. Pearson’s correlation coefficient between the HPLC and Reveal-Q+ data (0.45), although significant (P<0.0003), was lower than that obtained between HPLC and the FTIR method (0.94, P<0.0001). Both methods were considered efficient in quantifying DON levels in wheat-flour samples. This study was aimed at assisting the producers in choosing an appropriate tool for the purpose of analysis and upon consideration of the available equipment.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI)2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/79428enginfo:eu-repo/semantics/altIdentifier/issn/1875-0796info:eu-repo/semantics/altIdentifier/doi/10.3920/WMJ2016.2121info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:06:24Zoai:sedici.unlp.edu.ar:10915/79428Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:06:24.438SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
title Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
spellingShingle Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
Astoreca, Andrea Luciana
Química
deoxynivalenol
wheat
high-performance liquid chromatography
Fourier-transform infrared spectroscopy
lateral-flow immunochromatography
title_short Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
title_full Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
title_fullStr Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
title_full_unstemmed Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
title_sort Analytical techniques for deoxynivalenol detection and quantification in wheat destined for the manufacture of commercial products
dc.creator.none.fl_str_mv Astoreca, Andrea Luciana
Ortega, Leonel Maximiliano
Fígoli, Cecilia Beatriz
Cardós, M.
Cavaglieri, L.
Bosch, María Alejandra
Alconada Magliano, Teresa María
author Astoreca, Andrea Luciana
author_facet Astoreca, Andrea Luciana
Ortega, Leonel Maximiliano
Fígoli, Cecilia Beatriz
Cardós, M.
Cavaglieri, L.
Bosch, María Alejandra
Alconada Magliano, Teresa María
author_role author
author2 Ortega, Leonel Maximiliano
Fígoli, Cecilia Beatriz
Cardós, M.
Cavaglieri, L.
Bosch, María Alejandra
Alconada Magliano, Teresa María
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Química
deoxynivalenol
wheat
high-performance liquid chromatography
Fourier-transform infrared spectroscopy
lateral-flow immunochromatography
topic Química
deoxynivalenol
wheat
high-performance liquid chromatography
Fourier-transform infrared spectroscopy
lateral-flow immunochromatography
dc.description.none.fl_txt_mv The concern regarding toxicity from the presence of deoxynivalenol (DON) in wheat that affects both economy and public health leads to the need to find appropriate detection methods for determining the degree of DON contamination in terms of the equipment available and the speed required for obtaining the incidence. The objective of this study was to compare the performance of two alternative analytical techniques for DON quantification for use in the food industry with a reference technique. Samples of wheat and the commercial by-products were analysed by high-performance liquid chromatography (HPLC) with an ultraviolet detector as the reference method and the results compared with those obtained from a rapid lateral-flow immunochromatographic device (Reveal Q+) and of a Fourier-transform-infrared (FTIR) spectroscopy technique. Pearson’s correlation coefficient between the HPLC and Reveal-Q+ data (0.45), although significant (P<0.0003), was lower than that obtained between HPLC and the FTIR method (0.94, P<0.0001). Both methods were considered efficient in quantifying DON levels in wheat-flour samples. This study was aimed at assisting the producers in choosing an appropriate tool for the purpose of analysis and upon consideration of the available equipment.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI)
description The concern regarding toxicity from the presence of deoxynivalenol (DON) in wheat that affects both economy and public health leads to the need to find appropriate detection methods for determining the degree of DON contamination in terms of the equipment available and the speed required for obtaining the incidence. The objective of this study was to compare the performance of two alternative analytical techniques for DON quantification for use in the food industry with a reference technique. Samples of wheat and the commercial by-products were analysed by high-performance liquid chromatography (HPLC) with an ultraviolet detector as the reference method and the results compared with those obtained from a rapid lateral-flow immunochromatographic device (Reveal Q+) and of a Fourier-transform-infrared (FTIR) spectroscopy technique. Pearson’s correlation coefficient between the HPLC and Reveal-Q+ data (0.45), although significant (P<0.0003), was lower than that obtained between HPLC and the FTIR method (0.94, P<0.0001). Both methods were considered efficient in quantifying DON levels in wheat-flour samples. This study was aimed at assisting the producers in choosing an appropriate tool for the purpose of analysis and upon consideration of the available equipment.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/79428
url http://sedici.unlp.edu.ar/handle/10915/79428
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1875-0796
info:eu-repo/semantics/altIdentifier/doi/10.3920/WMJ2016.2121
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1846064119216603136
score 12.749369

Publicaciones similares