Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays

Autores
Siano, Gabriel German; López Márquez, Juan Manuel; Pujato, Nazarena; Berli, Claudio Luis Alberto; Peretti, Leandro Ezequiel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Lateral flow immunoassays (LFI) are widely used worldwide for the detection of different analytes because they combine multiple advantages such as low production cost, simplicity, and portability, which allows biomarkers detection without requiring infrastructure or highly trained personnel. This work aims to provide solutions to some steps of the LFI manufacturing process, particularly the controlled dispensing of reagents in the form of test lines. Automating this stage of LFI production provides greater control over the amounts of reagents deposited and greater reproducibility between and within batches. In this work, a Cartesian 3D printer was adapted and syringe pumps controlled by the printer itself were used. Thus, the control of their speeds, together with the output calibers in the dispensing tips and with the specific volume of each syringe, allowed optimizing the widths of the lines. As an application case, antigens of Leptospira interrogans, causal agent of Leptospirosis, were dispensed on nitrocellulose membranes, and the recognition reaction was tested. The results obtained indicate that it is indeed possible to automate dispensing tasks with the proposed system, reaching quality levels similar to those obtained with commercial instruments, but at a much lower cost, and with a higher adaptation and configuration capacity. This makes it possible to avoid manual seeding, taking better advantage of previous immunochromatographic optimizations and facilitating the development and lab-scale production steps of LFI.
Fil: Siano, Gabriel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas. Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: López Márquez, Juan Manuel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Peretti, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Médicas; Argentina
III Brazil-Argentine Microfluidics Congress; VI Congreso de Microfluídica Argentina
Buenos Aires
Argentina
Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes
Agencia Nacional de Promoción Científica y Tecnológica
Consejo Nacional de Investigaciones Científicas y Técnicas
Materia
DISPENSING
3D PRINTER
LATERAL FLOW IMMUNOASSAYS
IMMUNOCHROMATOGRAPHY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237169
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassaysSiano, Gabriel GermanLópez Márquez, Juan ManuelPujato, NazarenaBerli, Claudio Luis AlbertoPeretti, Leandro EzequielDISPENSING3D PRINTERLATERAL FLOW IMMUNOASSAYSIMMUNOCHROMATOGRAPHYhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Lateral flow immunoassays (LFI) are widely used worldwide for the detection of different analytes because they combine multiple advantages such as low production cost, simplicity, and portability, which allows biomarkers detection without requiring infrastructure or highly trained personnel. This work aims to provide solutions to some steps of the LFI manufacturing process, particularly the controlled dispensing of reagents in the form of test lines. Automating this stage of LFI production provides greater control over the amounts of reagents deposited and greater reproducibility between and within batches. In this work, a Cartesian 3D printer was adapted and syringe pumps controlled by the printer itself were used. Thus, the control of their speeds, together with the output calibers in the dispensing tips and with the specific volume of each syringe, allowed optimizing the widths of the lines. As an application case, antigens of Leptospira interrogans, causal agent of Leptospirosis, were dispensed on nitrocellulose membranes, and the recognition reaction was tested. The results obtained indicate that it is indeed possible to automate dispensing tasks with the proposed system, reaching quality levels similar to those obtained with commercial instruments, but at a much lower cost, and with a higher adaptation and configuration capacity. This makes it possible to avoid manual seeding, taking better advantage of previous immunochromatographic optimizations and facilitating the development and lab-scale production steps of LFI.Fil: Siano, Gabriel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas. Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: López Márquez, Juan Manuel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; ArgentinaFil: Peretti, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Médicas; ArgentinaIII Brazil-Argentine Microfluidics Congress; VI Congreso de Microfluídica ArgentinaBuenos AiresArgentinaComisión Nacional de Energía Atómica. Centro Atómico ConstituyentesAgencia Nacional de Promoción Científica y TecnológicaConsejo Nacional de Investigaciones Científicas y TécnicasComisión Nacional de Energía Atómica. Centro Atómico Constituyentes2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237169Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays; III Brazil-Argentine Microfluidics Congress; VI Congreso de Microfluídica Argentina; Buenos Aires; Argentina; 2022; 60-60CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sites.google.com/view/microfluidics2022/book-of-abstractsInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:14Zoai:ri.conicet.gov.ar:11336/237169instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:14.399CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays
title Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays
spellingShingle Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays
Siano, Gabriel German
DISPENSING
3D PRINTER
LATERAL FLOW IMMUNOASSAYS
IMMUNOCHROMATOGRAPHY
title_short Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays
title_full Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays
title_fullStr Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays
title_full_unstemmed Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays
title_sort Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays
dc.creator.none.fl_str_mv Siano, Gabriel German
López Márquez, Juan Manuel
Pujato, Nazarena
Berli, Claudio Luis Alberto
Peretti, Leandro Ezequiel
author Siano, Gabriel German
author_facet Siano, Gabriel German
López Márquez, Juan Manuel
Pujato, Nazarena
Berli, Claudio Luis Alberto
Peretti, Leandro Ezequiel
author_role author
author2 López Márquez, Juan Manuel
Pujato, Nazarena
Berli, Claudio Luis Alberto
Peretti, Leandro Ezequiel
author2_role author
author
author
author
dc.subject.none.fl_str_mv DISPENSING
3D PRINTER
LATERAL FLOW IMMUNOASSAYS
IMMUNOCHROMATOGRAPHY
topic DISPENSING
3D PRINTER
LATERAL FLOW IMMUNOASSAYS
IMMUNOCHROMATOGRAPHY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Lateral flow immunoassays (LFI) are widely used worldwide for the detection of different analytes because they combine multiple advantages such as low production cost, simplicity, and portability, which allows biomarkers detection without requiring infrastructure or highly trained personnel. This work aims to provide solutions to some steps of the LFI manufacturing process, particularly the controlled dispensing of reagents in the form of test lines. Automating this stage of LFI production provides greater control over the amounts of reagents deposited and greater reproducibility between and within batches. In this work, a Cartesian 3D printer was adapted and syringe pumps controlled by the printer itself were used. Thus, the control of their speeds, together with the output calibers in the dispensing tips and with the specific volume of each syringe, allowed optimizing the widths of the lines. As an application case, antigens of Leptospira interrogans, causal agent of Leptospirosis, were dispensed on nitrocellulose membranes, and the recognition reaction was tested. The results obtained indicate that it is indeed possible to automate dispensing tasks with the proposed system, reaching quality levels similar to those obtained with commercial instruments, but at a much lower cost, and with a higher adaptation and configuration capacity. This makes it possible to avoid manual seeding, taking better advantage of previous immunochromatographic optimizations and facilitating the development and lab-scale production steps of LFI.
Fil: Siano, Gabriel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas. Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: López Márquez, Juan Manuel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Berli, Claudio Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Peretti, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Médicas; Argentina
III Brazil-Argentine Microfluidics Congress; VI Congreso de Microfluídica Argentina
Buenos Aires
Argentina
Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes
Agencia Nacional de Promoción Científica y Tecnológica
Consejo Nacional de Investigaciones Científicas y Técnicas
description Lateral flow immunoassays (LFI) are widely used worldwide for the detection of different analytes because they combine multiple advantages such as low production cost, simplicity, and portability, which allows biomarkers detection without requiring infrastructure or highly trained personnel. This work aims to provide solutions to some steps of the LFI manufacturing process, particularly the controlled dispensing of reagents in the form of test lines. Automating this stage of LFI production provides greater control over the amounts of reagents deposited and greater reproducibility between and within batches. In this work, a Cartesian 3D printer was adapted and syringe pumps controlled by the printer itself were used. Thus, the control of their speeds, together with the output calibers in the dispensing tips and with the specific volume of each syringe, allowed optimizing the widths of the lines. As an application case, antigens of Leptospira interrogans, causal agent of Leptospirosis, were dispensed on nitrocellulose membranes, and the recognition reaction was tested. The results obtained indicate that it is indeed possible to automate dispensing tasks with the proposed system, reaching quality levels similar to those obtained with commercial instruments, but at a much lower cost, and with a higher adaptation and configuration capacity. This makes it possible to avoid manual seeding, taking better advantage of previous immunochromatographic optimizations and facilitating the development and lab-scale production steps of LFI.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237169
Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays; III Brazil-Argentine Microfluidics Congress; VI Congreso de Microfluídica Argentina; Buenos Aires; Argentina; 2022; 60-60
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237169
identifier_str_mv Controlled deposition of reagents with 3D printer for the manufacture of lateral flow immunoassays; III Brazil-Argentine Microfluidics Congress; VI Congreso de Microfluídica Argentina; Buenos Aires; Argentina; 2022; 60-60
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sites.google.com/view/microfluidics2022/book-of-abstracts
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes
publisher.none.fl_str_mv Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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