Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies

Autores
Torres, María Luz; Hurtado Cuba, Angel; Oberti, Tamara Gisela; Fernández, Juan Manuel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the present work, the osteogenic and angiogenic properties of, previously developed, semi-interpenetrated HEMA-EGDMA polymeric networks (sIPN) with and without alginate with application in bone tissue engineering (BTE) were studied. In vitro characterization studies were performed using rat bone marrow progenitor cells (BMPCs), EA.hy926 endothelial cells, and rat vascular smooth muscle cells (VSMCs). Based on the in vitro results of both this work and previous ones, the hydrogels were selected to carry out in vivo studies to find out their capacity as a biomaterial using a bone regeneration model. Our results indicate that the incorporation of alginate into the HEMA-EGDMA polymeric network promotes osteogenic and angiogenic capacity in cell cultures of BMPCs and both EA.hy926 and VSMCs, respectively, and also increases bone formation and vascular structures in in vivo studies, demonstrating its potential use as a biomaterial in BTE.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
Materia
Química
Angiogenesis
Biomaterial oseo
Evaluación in vivo
Diferenciación osteoblastica
Red polimérica semi-interpenetrada
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/191941
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/191941
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repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo StudiesTorres, María LuzHurtado Cuba, AngelOberti, Tamara GiselaFernández, Juan ManuelQuímicaAngiogenesisBiomaterial oseoEvaluación in vivoDiferenciación osteoblasticaRed polimérica semi-interpenetradaIn the present work, the osteogenic and angiogenic properties of, previously developed, semi-interpenetrated HEMA-EGDMA polymeric networks (sIPN) with and without alginate with application in bone tissue engineering (BTE) were studied. In vitro characterization studies were performed using rat bone marrow progenitor cells (BMPCs), EA.hy926 endothelial cells, and rat vascular smooth muscle cells (VSMCs). Based on the in vitro results of both this work and previous ones, the hydrogels were selected to carry out in vivo studies to find out their capacity as a biomaterial using a bone regeneration model. Our results indicate that the incorporation of alginate into the HEMA-EGDMA polymeric network promotes osteogenic and angiogenic capacity in cell cultures of BMPCs and both EA.hy926 and VSMCs, respectively, and also increases bone formation and vascular structures in in vivo studies, demonstrating its potential use as a biomaterial in BTE.Instituto de Investigaciones Fisicoquímicas Teóricas y AplicadasLaboratorio de Investigación en Osteopatías y Metabolismo Mineral2024-10-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/191941enginfo:eu-repo/semantics/altIdentifier/issn/1552-4973info:eu-repo/semantics/altIdentifier/issn/1552-4981info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.b.35493info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2026-04-23T11:55:39Zoai:sedici.unlp.edu.ar:10915/191941Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292026-04-23 11:55:39.704SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies
title Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies
spellingShingle Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies
Torres, María Luz
Química
Angiogenesis
Biomaterial oseo
Evaluación in vivo
Diferenciación osteoblastica
Red polimérica semi-interpenetrada
title_short Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies
title_full Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies
title_fullStr Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies
title_full_unstemmed Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies
title_sort Alginate-HEMA Hydrogels as Promising Biomaterials for Bone Regeneration: In Vitro and In Vivo Studies
dc.creator.none.fl_str_mv Torres, María Luz
Hurtado Cuba, Angel
Oberti, Tamara Gisela
Fernández, Juan Manuel
author Torres, María Luz
author_facet Torres, María Luz
Hurtado Cuba, Angel
Oberti, Tamara Gisela
Fernández, Juan Manuel
author_role author
author2 Hurtado Cuba, Angel
Oberti, Tamara Gisela
Fernández, Juan Manuel
author2_role author
author
author
dc.subject.none.fl_str_mv Química
Angiogenesis
Biomaterial oseo
Evaluación in vivo
Diferenciación osteoblastica
Red polimérica semi-interpenetrada
topic Química
Angiogenesis
Biomaterial oseo
Evaluación in vivo
Diferenciación osteoblastica
Red polimérica semi-interpenetrada
dc.description.none.fl_txt_mv In the present work, the osteogenic and angiogenic properties of, previously developed, semi-interpenetrated HEMA-EGDMA polymeric networks (sIPN) with and without alginate with application in bone tissue engineering (BTE) were studied. In vitro characterization studies were performed using rat bone marrow progenitor cells (BMPCs), EA.hy926 endothelial cells, and rat vascular smooth muscle cells (VSMCs). Based on the in vitro results of both this work and previous ones, the hydrogels were selected to carry out in vivo studies to find out their capacity as a biomaterial using a bone regeneration model. Our results indicate that the incorporation of alginate into the HEMA-EGDMA polymeric network promotes osteogenic and angiogenic capacity in cell cultures of BMPCs and both EA.hy926 and VSMCs, respectively, and also increases bone formation and vascular structures in in vivo studies, demonstrating its potential use as a biomaterial in BTE.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
description In the present work, the osteogenic and angiogenic properties of, previously developed, semi-interpenetrated HEMA-EGDMA polymeric networks (sIPN) with and without alginate with application in bone tissue engineering (BTE) were studied. In vitro characterization studies were performed using rat bone marrow progenitor cells (BMPCs), EA.hy926 endothelial cells, and rat vascular smooth muscle cells (VSMCs). Based on the in vitro results of both this work and previous ones, the hydrogels were selected to carry out in vivo studies to find out their capacity as a biomaterial using a bone regeneration model. Our results indicate that the incorporation of alginate into the HEMA-EGDMA polymeric network promotes osteogenic and angiogenic capacity in cell cultures of BMPCs and both EA.hy926 and VSMCs, respectively, and also increases bone formation and vascular structures in in vivo studies, demonstrating its potential use as a biomaterial in BTE.
publishDate 2024
dc.date.none.fl_str_mv 2024-10-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/191941
url http://sedici.unlp.edu.ar/handle/10915/191941
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1552-4973
info:eu-repo/semantics/altIdentifier/issn/1552-4981
info:eu-repo/semantics/altIdentifier/doi/10.1002/jbm.b.35493
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.05261

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