Hepatic Δ9, Δ6, and Δ5 desaturations in non-insulin-dependent diabetes mellitus eSS rats

Autores
Montanaro, Mauro Aldo; Rimoldi, Omar Jorge; Igal, Rubén Ariel; Montenegro, Silvana Marisa; Tarrés, María Cristina; Martínez, Stella Maris; Brenner, Rodolfo Roberto
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Both diabetes mellitus type 1 and diabetes mellitus type 2 are widespread diseases that alter carbohydrate and lipid metabolism. e Stilmann-Salgado (eSS) rats are experimental animals that spontaneously evolve to a state similar to that of young people affected by non-insulin-dependent diabetes mellitus (NIDDM; type 2). Using 6-mon-old eSS rats that, according to the literature [Martinez, S.M., Tarres, M.C., Montenegro, S., Milo, R., Picena, J.C., Figueroa, N., and Rabasa, S.R. (1988) Spontaneous Diabetes in eSS Rats, Acta Diabetol. Lat. 25, 303-313], had already developed insulin resistance, we investigated the changes evoked on delta9, delta6, and delta5 liver desaturases. The abundance of mRNA and enzymatic activities were measured, as well as the FA composition of liver microsomal lipids. Compared to control rats, the mRNA content and activity of SCD-1 (stearoyl CoA-desaturase, isoform of the delta9 desaturase) were significantly higher, whereas the mRNA and activities of delta6 and delta5 desaturases were not significantly modified. Correspondingly, the proportion of 18:1n-9 and the ratios of 18:1n-9/18:0 and 16:1/16:0 in lipids were significantly increased, whereas the proportion of 20:4n-6 was unaltered. These effects were found while glycemia was constant or increased. The results are completely opposite those described in insulin-dependent diabetes mellitus (type 1), in which a depression of all the desaturases is found. They suggest that in eSS rats, the activities of the desaturases were not modified by an insulin-resistance effect. Moreover, we suggest that the enhancement of SCD-1 activity might be considered as another typical sign of the NIDDM syndrome, because it has also been found in other animal models of NIDDM, for example, the ones evoked by the sucrose-rich diet and in the Zucker rat.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Ciencias Médicas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
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oai:sedici.unlp.edu.ar:10915/145036

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spelling Hepatic Δ9, Δ6, and Δ5 desaturations in non-insulin-dependent diabetes mellitus eSS ratsMontanaro, Mauro AldoRimoldi, Omar JorgeIgal, Rubén ArielMontenegro, Silvana MarisaTarrés, María CristinaMartínez, Stella MarisBrenner, Rodolfo RobertoCiencias MédicasBoth diabetes mellitus type 1 and diabetes mellitus type 2 are widespread diseases that alter carbohydrate and lipid metabolism. e Stilmann-Salgado (eSS) rats are experimental animals that spontaneously evolve to a state similar to that of young people affected by non-insulin-dependent diabetes mellitus (NIDDM; type 2). Using 6-mon-old eSS rats that, according to the literature [Martinez, S.M., Tarres, M.C., Montenegro, S., Milo, R., Picena, J.C., Figueroa, N., and Rabasa, S.R. (1988) Spontaneous Diabetes in eSS Rats, Acta Diabetol. Lat. 25, 303-313], had already developed insulin resistance, we investigated the changes evoked on delta9, delta6, and delta5 liver desaturases. The abundance of mRNA and enzymatic activities were measured, as well as the FA composition of liver microsomal lipids. Compared to control rats, the mRNA content and activity of SCD-1 (stearoyl CoA-desaturase, isoform of the delta9 desaturase) were significantly higher, whereas the mRNA and activities of delta6 and delta5 desaturases were not significantly modified. Correspondingly, the proportion of 18:1n-9 and the ratios of 18:1n-9/18:0 and 16:1/16:0 in lipids were significantly increased, whereas the proportion of 20:4n-6 was unaltered. These effects were found while glycemia was constant or increased. The results are completely opposite those described in insulin-dependent diabetes mellitus (type 1), in which a depression of all the desaturases is found. They suggest that in eSS rats, the activities of the desaturases were not modified by an insulin-resistance effect. Moreover, we suggest that the enhancement of SCD-1 activity might be considered as another typical sign of the NIDDM syndrome, because it has also been found in other animal models of NIDDM, for example, the ones evoked by the sucrose-rich diet and in the Zucker rat.Instituto de Investigaciones Bioquímicas de La Plata2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf827-832http://sedici.unlp.edu.ar/handle/10915/145036enginfo:eu-repo/semantics/altIdentifier/issn/0024-4201info:eu-repo/semantics/altIdentifier/issn/1558-9307info:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-003-1132-7info:eu-repo/semantics/altIdentifier/pmid/14577661info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:12Zoai:sedici.unlp.edu.ar:10915/145036Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:13.193SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Hepatic Δ9, Δ6, and Δ5 desaturations in non-insulin-dependent diabetes mellitus eSS rats
title Hepatic Δ9, Δ6, and Δ5 desaturations in non-insulin-dependent diabetes mellitus eSS rats
spellingShingle Hepatic Δ9, Δ6, and Δ5 desaturations in non-insulin-dependent diabetes mellitus eSS rats
Montanaro, Mauro Aldo
Ciencias Médicas
title_short Hepatic Δ9, Δ6, and Δ5 desaturations in non-insulin-dependent diabetes mellitus eSS rats
title_full Hepatic Δ9, Δ6, and Δ5 desaturations in non-insulin-dependent diabetes mellitus eSS rats
title_fullStr Hepatic Δ9, Δ6, and Δ5 desaturations in non-insulin-dependent diabetes mellitus eSS rats
title_full_unstemmed Hepatic Δ9, Δ6, and Δ5 desaturations in non-insulin-dependent diabetes mellitus eSS rats
title_sort Hepatic Δ9, Δ6, and Δ5 desaturations in non-insulin-dependent diabetes mellitus eSS rats
dc.creator.none.fl_str_mv Montanaro, Mauro Aldo
Rimoldi, Omar Jorge
Igal, Rubén Ariel
Montenegro, Silvana Marisa
Tarrés, María Cristina
Martínez, Stella Maris
Brenner, Rodolfo Roberto
author Montanaro, Mauro Aldo
author_facet Montanaro, Mauro Aldo
Rimoldi, Omar Jorge
Igal, Rubén Ariel
Montenegro, Silvana Marisa
Tarrés, María Cristina
Martínez, Stella Maris
Brenner, Rodolfo Roberto
author_role author
author2 Rimoldi, Omar Jorge
Igal, Rubén Ariel
Montenegro, Silvana Marisa
Tarrés, María Cristina
Martínez, Stella Maris
Brenner, Rodolfo Roberto
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
topic Ciencias Médicas
dc.description.none.fl_txt_mv Both diabetes mellitus type 1 and diabetes mellitus type 2 are widespread diseases that alter carbohydrate and lipid metabolism. e Stilmann-Salgado (eSS) rats are experimental animals that spontaneously evolve to a state similar to that of young people affected by non-insulin-dependent diabetes mellitus (NIDDM; type 2). Using 6-mon-old eSS rats that, according to the literature [Martinez, S.M., Tarres, M.C., Montenegro, S., Milo, R., Picena, J.C., Figueroa, N., and Rabasa, S.R. (1988) Spontaneous Diabetes in eSS Rats, Acta Diabetol. Lat. 25, 303-313], had already developed insulin resistance, we investigated the changes evoked on delta9, delta6, and delta5 liver desaturases. The abundance of mRNA and enzymatic activities were measured, as well as the FA composition of liver microsomal lipids. Compared to control rats, the mRNA content and activity of SCD-1 (stearoyl CoA-desaturase, isoform of the delta9 desaturase) were significantly higher, whereas the mRNA and activities of delta6 and delta5 desaturases were not significantly modified. Correspondingly, the proportion of 18:1n-9 and the ratios of 18:1n-9/18:0 and 16:1/16:0 in lipids were significantly increased, whereas the proportion of 20:4n-6 was unaltered. These effects were found while glycemia was constant or increased. The results are completely opposite those described in insulin-dependent diabetes mellitus (type 1), in which a depression of all the desaturases is found. They suggest that in eSS rats, the activities of the desaturases were not modified by an insulin-resistance effect. Moreover, we suggest that the enhancement of SCD-1 activity might be considered as another typical sign of the NIDDM syndrome, because it has also been found in other animal models of NIDDM, for example, the ones evoked by the sucrose-rich diet and in the Zucker rat.
Instituto de Investigaciones Bioquímicas de La Plata
description Both diabetes mellitus type 1 and diabetes mellitus type 2 are widespread diseases that alter carbohydrate and lipid metabolism. e Stilmann-Salgado (eSS) rats are experimental animals that spontaneously evolve to a state similar to that of young people affected by non-insulin-dependent diabetes mellitus (NIDDM; type 2). Using 6-mon-old eSS rats that, according to the literature [Martinez, S.M., Tarres, M.C., Montenegro, S., Milo, R., Picena, J.C., Figueroa, N., and Rabasa, S.R. (1988) Spontaneous Diabetes in eSS Rats, Acta Diabetol. Lat. 25, 303-313], had already developed insulin resistance, we investigated the changes evoked on delta9, delta6, and delta5 liver desaturases. The abundance of mRNA and enzymatic activities were measured, as well as the FA composition of liver microsomal lipids. Compared to control rats, the mRNA content and activity of SCD-1 (stearoyl CoA-desaturase, isoform of the delta9 desaturase) were significantly higher, whereas the mRNA and activities of delta6 and delta5 desaturases were not significantly modified. Correspondingly, the proportion of 18:1n-9 and the ratios of 18:1n-9/18:0 and 16:1/16:0 in lipids were significantly increased, whereas the proportion of 20:4n-6 was unaltered. These effects were found while glycemia was constant or increased. The results are completely opposite those described in insulin-dependent diabetes mellitus (type 1), in which a depression of all the desaturases is found. They suggest that in eSS rats, the activities of the desaturases were not modified by an insulin-resistance effect. Moreover, we suggest that the enhancement of SCD-1 activity might be considered as another typical sign of the NIDDM syndrome, because it has also been found in other animal models of NIDDM, for example, the ones evoked by the sucrose-rich diet and in the Zucker rat.
publishDate 2003
dc.date.none.fl_str_mv 2003
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info:eu-repo/semantics/altIdentifier/issn/1558-9307
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11745-003-1132-7
info:eu-repo/semantics/altIdentifier/pmid/14577661
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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