Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?

Autores
Naum-Onganía, Gabriela; Díaz Cortés, Víctor Manuel; Blasi, Francesco; Rivera Pomar, Rolando Víctor
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WAS P, and transcription/splicing factors p54nrb and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles.
Centro Regional de Estudios Genómicos
Materia
Biología
Ciencias Naturales
Co-transcriptional splicing
Hox genes
Nuclear actin
Nuclear speckles
Rna pol ii
Transcription/splicing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/96269
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/96269
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network_name_str SEDICI (UNLP)
spelling Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?Naum-Onganía, GabrielaDíaz Cortés, Víctor ManuelBlasi, FrancescoRivera Pomar, Rolando VíctorBiologíaCiencias NaturalesCo-transcriptional splicingHox genesNuclear actinNuclear specklesRna pol iiTranscription/splicingThe HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WAS P, and transcription/splicing factors p54<sup>nrb</sup> and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles.Centro Regional de Estudios Genómicos2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf260-272http://sedici.unlp.edu.ar/handle/10915/96269enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/75838info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.4161/trns.27672info:eu-repo/semantics/altIdentifier/issn/1949-1034info:eu-repo/semantics/altIdentifier/doi/10.4161/trns.27672info:eu-repo/semantics/altIdentifier/hdl/11336/75838info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/ar/Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Argentina (CC BY-NC-SA 2.5)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:21Zoai:sedici.unlp.edu.ar:10915/96269Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:22.139SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?
title Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?
spellingShingle Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?
Naum-Onganía, Gabriela
Biología
Ciencias Naturales
Co-transcriptional splicing
Hox genes
Nuclear actin
Nuclear speckles
Rna pol ii
Transcription/splicing
title_short Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?
title_full Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?
title_fullStr Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?
title_full_unstemmed Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?
title_sort Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription : Are nuclear speckles involved?
dc.creator.none.fl_str_mv Naum-Onganía, Gabriela
Díaz Cortés, Víctor Manuel
Blasi, Francesco
Rivera Pomar, Rolando Víctor
author Naum-Onganía, Gabriela
author_facet Naum-Onganía, Gabriela
Díaz Cortés, Víctor Manuel
Blasi, Francesco
Rivera Pomar, Rolando Víctor
author_role author
author2 Díaz Cortés, Víctor Manuel
Blasi, Francesco
Rivera Pomar, Rolando Víctor
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
Ciencias Naturales
Co-transcriptional splicing
Hox genes
Nuclear actin
Nuclear speckles
Rna pol ii
Transcription/splicing
topic Biología
Ciencias Naturales
Co-transcriptional splicing
Hox genes
Nuclear actin
Nuclear speckles
Rna pol ii
Transcription/splicing
dc.description.none.fl_txt_mv The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WAS P, and transcription/splicing factors p54<sup>nrb</sup> and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles.
Centro Regional de Estudios Genómicos
description The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WAS P, and transcription/splicing factors p54<sup>nrb</sup> and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/96269
url http://sedici.unlp.edu.ar/handle/10915/96269
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/75838
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.4161/trns.27672
info:eu-repo/semantics/altIdentifier/issn/1949-1034
info:eu-repo/semantics/altIdentifier/doi/10.4161/trns.27672
info:eu-repo/semantics/altIdentifier/hdl/11336/75838
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Argentina (CC BY-NC-SA 2.5)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Argentina (CC BY-NC-SA 2.5)
dc.format.none.fl_str_mv application/pdf
260-272
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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