Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?

Autores
Naum Ongania, Gabriela; Díaz Cortés, Víctor Manuel; Blasi, Francesco; Rivera Pomar, Rolando
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WASP, and transcription/splicing factors p54nrb and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles.
Fil: Naum Ongania, Gabriela. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz Cortés, Víctor Manuel. Institut Municipal d’Investigació Medica. Barcelona; España
Fil: Blasi, Francesco. Istituto FIRC di Oncologia Molecolare. Milan; Italia
Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Nuclear Actin
Nuclear Speckles
Co-Transcriptional Splicing
Rna Pol Ii
Transcription/Splicing
Hox Genes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24638
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24638
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?Naum Ongania, GabrielaDíaz Cortés, Víctor ManuelBlasi, FrancescoRivera Pomar, RolandoNuclear ActinNuclear SpecklesCo-Transcriptional SplicingRna Pol IiTranscription/SplicingHox Geneshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WASP, and transcription/splicing factors p54nrb and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles.Fil: Naum Ongania, Gabriela. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Díaz Cortés, Víctor Manuel. Institut Municipal d’Investigació Medica. Barcelona; EspañaFil: Blasi, Francesco. Istituto FIRC di Oncologia Molecolare. Milan; ItaliaFil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaLandes Biosciences2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24638Naum Ongania, Gabriela; Díaz Cortés, Víctor Manuel; Blasi, Francesco; Rivera Pomar, Rolando; Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?; Landes Biosciences; Transcription; 4; 5; 1-2014; 260-2722154-12642154-1272CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4161/trns.27672info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.4161/trns.27672info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:29:14Zoai:ri.conicet.gov.ar:11336/24638instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:29:15.194CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?
title Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?
spellingShingle Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?
Naum Ongania, Gabriela
Nuclear Actin
Nuclear Speckles
Co-Transcriptional Splicing
Rna Pol Ii
Transcription/Splicing
Hox Genes
title_short Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?
title_full Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?
title_fullStr Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?
title_full_unstemmed Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?
title_sort Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?
dc.creator.none.fl_str_mv Naum Ongania, Gabriela
Díaz Cortés, Víctor Manuel
Blasi, Francesco
Rivera Pomar, Rolando
author Naum Ongania, Gabriela
author_facet Naum Ongania, Gabriela
Díaz Cortés, Víctor Manuel
Blasi, Francesco
Rivera Pomar, Rolando
author_role author
author2 Díaz Cortés, Víctor Manuel
Blasi, Francesco
Rivera Pomar, Rolando
author2_role author
author
author
dc.subject.none.fl_str_mv Nuclear Actin
Nuclear Speckles
Co-Transcriptional Splicing
Rna Pol Ii
Transcription/Splicing
Hox Genes
topic Nuclear Actin
Nuclear Speckles
Co-Transcriptional Splicing
Rna Pol Ii
Transcription/Splicing
Hox Genes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WASP, and transcription/splicing factors p54nrb and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles.
Fil: Naum Ongania, Gabriela. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz Cortés, Víctor Manuel. Institut Municipal d’Investigació Medica. Barcelona; España
Fil: Blasi, Francesco. Istituto FIRC di Oncologia Molecolare. Milan; Italia
Fil: Rivera Pomar, Rolando. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The HoxB cluster expression is activated by retinoic acid and transcribed in a collinear manner. The DNA-binding Pknox1-Pbx1 complex modulates Hox protein activity. Here, NT2-D1 teratocarcinoma cells -a model of Hox gene expression- were used to show that upon retinoic acid induction, Pknox1 co-localizes with polymeric nuclear actin. We have found that globular actin aggregates, polymeric actin, the elongating RNA polymerase II and THOC match euchromatic regions corresponding to nuclear speckles. Moreover, RNA polymerase II, N-WASP, and transcription/splicing factors p54nrb and PSF were validated as Pknox1 interactors by tandem affinity purification. PSF pulled down with THOC and nuclear actin, both of which co-localize in nuclear speckles. Although latrunculin A slightly decreases the general level of HoxB gene expression, inhibition of nuclear actin polymerization by cytochalasin D blocks the expression of HoxB transcripts in a collinear manner. Thus, our results support the hypothesis that nuclear actin polymerization is involved in the activation of HoxB gene expression by means of nuclear speckles.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24638
Naum Ongania, Gabriela; Díaz Cortés, Víctor Manuel; Blasi, Francesco; Rivera Pomar, Rolando; Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?; Landes Biosciences; Transcription; 4; 5; 1-2014; 260-272
2154-1264
2154-1272
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24638
identifier_str_mv Naum Ongania, Gabriela; Díaz Cortés, Víctor Manuel; Blasi, Francesco; Rivera Pomar, Rolando; Nuclear actin polymerization from faster growing ends in the initial activation of hox gene transcription Are nuclear speckles involved?; Landes Biosciences; Transcription; 4; 5; 1-2014; 260-272
2154-1264
2154-1272
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4161/trns.27672
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.4161/trns.27672
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Landes Biosciences
publisher.none.fl_str_mv Landes Biosciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.084122

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