Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants

Autores
Lucero, Leandro Exequiel; Bazin, Jeremie; Rodriguez Melo, Johan Stiben; Ibañez, Fernando Julio; Crespi, Leandro Martín; Ariel, Federico Damian
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
RNA-Binding Protein 1 (RBP1) was first identified as a protein partner of the long noncoding RNA (lncRNA) ENOD40 in Medicago truncatula, involved in symbiotic nodule development. RBP1 is localized in nuclear speckles and can be relocalized to the cytoplasm by the interaction with ENOD40. The two closest homologs to RBP1 in Arabidopsis thaliana were called Nuclear Speckle RNA-binding proteins (NSRs) and characterized as alternative splicing modulators of specific mRNAs. They can recognize in vivo the lncRNA ALTERNATIVE SPLICING COMPETITOR (ASCO) among other lncRNAs, regulating lateral root formation. Here, we performed a phylogenetic analysis of NSR/RBP proteins tracking the roots of the family to the Embryophytes. Strikingly, eudicots faced a reductive trend of NSR/RBP proteins in comparison with other groups of flowering plants. In Medicago truncatula and Lotus japonicus, their expression profile during nodulation and in specific regions of the symbiotic nodule was compared to that of the lncRNA ENOD40, as well as to changes in alternative splicing. This hinted at distinct and specific roles of each member during nodulation, likely modulating the population of alternatively spliced transcripts. Our results establish the basis to guide future exploration of NSR/RBP function in alternative splicing regulation in different developmental contexts along the plant lineage.
Fil: Lucero, Leandro Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Bazin, Jeremie. Université Paris-sud; Francia. Centre National de la Recherche Scientifique; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Rodriguez Melo, Johan Stiben. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Ibañez, Fernando Julio. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Crespi, Leandro Martín. Centre National de la Recherche Scientifique; Francia. Institut National de la Recherche Agronomique; Francia. Université Paris-sud; Francia
Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
ALTERNATIVE SPLICING
EVOLUTION
LEGUMES
LNCRNA ENOD40
NUCLEAR SPECKLE RNA-BINDING PROTEINS
RBP1
SYMBIOTIC NODULE DEVELOPMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/123704
Acceder
id CONICETDig_524ce5e542b40f95394bcc66a786741d
oai_identifier_str oai:ri.conicet.gov.ar:11336/123704
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plantsLucero, Leandro ExequielBazin, JeremieRodriguez Melo, Johan StibenIbañez, Fernando JulioCrespi, Leandro MartínAriel, Federico DamianALTERNATIVE SPLICINGEVOLUTIONLEGUMESLNCRNA ENOD40NUCLEAR SPECKLE RNA-BINDING PROTEINSRBP1SYMBIOTIC NODULE DEVELOPMENThttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4RNA-Binding Protein 1 (RBP1) was first identified as a protein partner of the long noncoding RNA (lncRNA) ENOD40 in Medicago truncatula, involved in symbiotic nodule development. RBP1 is localized in nuclear speckles and can be relocalized to the cytoplasm by the interaction with ENOD40. The two closest homologs to RBP1 in Arabidopsis thaliana were called Nuclear Speckle RNA-binding proteins (NSRs) and characterized as alternative splicing modulators of specific mRNAs. They can recognize in vivo the lncRNA ALTERNATIVE SPLICING COMPETITOR (ASCO) among other lncRNAs, regulating lateral root formation. Here, we performed a phylogenetic analysis of NSR/RBP proteins tracking the roots of the family to the Embryophytes. Strikingly, eudicots faced a reductive trend of NSR/RBP proteins in comparison with other groups of flowering plants. In Medicago truncatula and Lotus japonicus, their expression profile during nodulation and in specific regions of the symbiotic nodule was compared to that of the lncRNA ENOD40, as well as to changes in alternative splicing. This hinted at distinct and specific roles of each member during nodulation, likely modulating the population of alternatively spliced transcripts. Our results establish the basis to guide future exploration of NSR/RBP function in alternative splicing regulation in different developmental contexts along the plant lineage.Fil: Lucero, Leandro Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Bazin, Jeremie. Université Paris-sud; Francia. Centre National de la Recherche Scientifique; Francia. Institut National de la Recherche Agronomique; FranciaFil: Rodriguez Melo, Johan Stiben. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Ibañez, Fernando Julio. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; ArgentinaFil: Crespi, Leandro Martín. Centre National de la Recherche Scientifique; Francia. Institut National de la Recherche Agronomique; Francia. Université Paris-sud; FranciaFil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaMolecular Diversity Preservation International2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/123704Lucero, Leandro Exequiel; Bazin, Jeremie; Rodriguez Melo, Johan Stiben; Ibañez, Fernando Julio; Crespi, Leandro Martín; et al.; Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants; Molecular Diversity Preservation International; Genes; 11; 2; 2-2020; 1-132073-4425CONICET DigitalCONICETenghttps://www.mdpi.com/journal/genes/special_issues/root_nodule_symbiosesinfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4425/11/2/207info:eu-repo/semantics/altIdentifier/doi/10.3390/genes11020207info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:35Zoai:ri.conicet.gov.ar:11336/123704instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:35.63CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants
title Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants
spellingShingle Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants
Lucero, Leandro Exequiel
ALTERNATIVE SPLICING
EVOLUTION
LEGUMES
LNCRNA ENOD40
NUCLEAR SPECKLE RNA-BINDING PROTEINS
RBP1
SYMBIOTIC NODULE DEVELOPMENT
title_short Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants
title_full Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants
title_fullStr Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants
title_full_unstemmed Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants
title_sort Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants
dc.creator.none.fl_str_mv Lucero, Leandro Exequiel
Bazin, Jeremie
Rodriguez Melo, Johan Stiben
Ibañez, Fernando Julio
Crespi, Leandro Martín
Ariel, Federico Damian
author Lucero, Leandro Exequiel
author_facet Lucero, Leandro Exequiel
Bazin, Jeremie
Rodriguez Melo, Johan Stiben
Ibañez, Fernando Julio
Crespi, Leandro Martín
Ariel, Federico Damian
author_role author
author2 Bazin, Jeremie
Rodriguez Melo, Johan Stiben
Ibañez, Fernando Julio
Crespi, Leandro Martín
Ariel, Federico Damian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ALTERNATIVE SPLICING
EVOLUTION
LEGUMES
LNCRNA ENOD40
NUCLEAR SPECKLE RNA-BINDING PROTEINS
RBP1
SYMBIOTIC NODULE DEVELOPMENT
topic ALTERNATIVE SPLICING
EVOLUTION
LEGUMES
LNCRNA ENOD40
NUCLEAR SPECKLE RNA-BINDING PROTEINS
RBP1
SYMBIOTIC NODULE DEVELOPMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv RNA-Binding Protein 1 (RBP1) was first identified as a protein partner of the long noncoding RNA (lncRNA) ENOD40 in Medicago truncatula, involved in symbiotic nodule development. RBP1 is localized in nuclear speckles and can be relocalized to the cytoplasm by the interaction with ENOD40. The two closest homologs to RBP1 in Arabidopsis thaliana were called Nuclear Speckle RNA-binding proteins (NSRs) and characterized as alternative splicing modulators of specific mRNAs. They can recognize in vivo the lncRNA ALTERNATIVE SPLICING COMPETITOR (ASCO) among other lncRNAs, regulating lateral root formation. Here, we performed a phylogenetic analysis of NSR/RBP proteins tracking the roots of the family to the Embryophytes. Strikingly, eudicots faced a reductive trend of NSR/RBP proteins in comparison with other groups of flowering plants. In Medicago truncatula and Lotus japonicus, their expression profile during nodulation and in specific regions of the symbiotic nodule was compared to that of the lncRNA ENOD40, as well as to changes in alternative splicing. This hinted at distinct and specific roles of each member during nodulation, likely modulating the population of alternatively spliced transcripts. Our results establish the basis to guide future exploration of NSR/RBP function in alternative splicing regulation in different developmental contexts along the plant lineage.
Fil: Lucero, Leandro Exequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Bazin, Jeremie. Université Paris-sud; Francia. Centre National de la Recherche Scientifique; Francia. Institut National de la Recherche Agronomique; Francia
Fil: Rodriguez Melo, Johan Stiben. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Ibañez, Fernando Julio. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones Agrobiotecnológicas - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Agrobiotecnológicas; Argentina
Fil: Crespi, Leandro Martín. Centre National de la Recherche Scientifique; Francia. Institut National de la Recherche Agronomique; Francia. Université Paris-sud; Francia
Fil: Ariel, Federico Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description RNA-Binding Protein 1 (RBP1) was first identified as a protein partner of the long noncoding RNA (lncRNA) ENOD40 in Medicago truncatula, involved in symbiotic nodule development. RBP1 is localized in nuclear speckles and can be relocalized to the cytoplasm by the interaction with ENOD40. The two closest homologs to RBP1 in Arabidopsis thaliana were called Nuclear Speckle RNA-binding proteins (NSRs) and characterized as alternative splicing modulators of specific mRNAs. They can recognize in vivo the lncRNA ALTERNATIVE SPLICING COMPETITOR (ASCO) among other lncRNAs, regulating lateral root formation. Here, we performed a phylogenetic analysis of NSR/RBP proteins tracking the roots of the family to the Embryophytes. Strikingly, eudicots faced a reductive trend of NSR/RBP proteins in comparison with other groups of flowering plants. In Medicago truncatula and Lotus japonicus, their expression profile during nodulation and in specific regions of the symbiotic nodule was compared to that of the lncRNA ENOD40, as well as to changes in alternative splicing. This hinted at distinct and specific roles of each member during nodulation, likely modulating the population of alternatively spliced transcripts. Our results establish the basis to guide future exploration of NSR/RBP function in alternative splicing regulation in different developmental contexts along the plant lineage.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/123704
Lucero, Leandro Exequiel; Bazin, Jeremie; Rodriguez Melo, Johan Stiben; Ibañez, Fernando Julio; Crespi, Leandro Martín; et al.; Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants; Molecular Diversity Preservation International; Genes; 11; 2; 2-2020; 1-13
2073-4425
CONICET Digital
CONICET
url http://hdl.handle.net/11336/123704
identifier_str_mv Lucero, Leandro Exequiel; Bazin, Jeremie; Rodriguez Melo, Johan Stiben; Ibañez, Fernando Julio; Crespi, Leandro Martín; et al.; Evolution of the small family of alternative splicing modulators nuclear speckle RNA-binding proteins in plants; Molecular Diversity Preservation International; Genes; 11; 2; 2-2020; 1-13
2073-4425
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.mdpi.com/journal/genes/special_issues/root_nodule_symbioses
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4425/11/2/207
info:eu-repo/semantics/altIdentifier/doi/10.3390/genes11020207
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614434651111424
score 13.070432

Publicaciones similares