Lectin-histochemistry: glycogenosis in cattle

Autores
Zlotowski, P.; Gimeno, Eduardo Juan; Díaz, A.; Barros, R.; Barros, Severo S.; Farias da Cruz, Claudio Estevao; Driemeier, D.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ten out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Brahman cattle
generalized glycogenosis
lectin histochemistry
lysosomal storage disorder
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/145221

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network_name_str SEDICI (UNLP)
spelling Lectin-histochemistry: glycogenosis in cattleZlotowski, P.Gimeno, Eduardo JuanDíaz, A.Barros, R.Barros, Severo S.Farias da Cruz, Claudio EstevaoDriemeier, D.Ciencias VeterinariasBrahman cattlegeneralized glycogenosislectin histochemistrylysosomal storage disorderTen out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis.Facultad de Ciencias Veterinarias2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf369-377http://sedici.unlp.edu.ar/handle/10915/145221enginfo:eu-repo/semantics/altIdentifier/issn/0165-7380info:eu-repo/semantics/altIdentifier/issn/1573-7446info:eu-repo/semantics/altIdentifier/doi/10.1007/s11259-006-3237-0info:eu-repo/semantics/altIdentifier/pmid/16502105info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:21Zoai:sedici.unlp.edu.ar:10915/145221Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:22.072SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Lectin-histochemistry: glycogenosis in cattle
title Lectin-histochemistry: glycogenosis in cattle
spellingShingle Lectin-histochemistry: glycogenosis in cattle
Zlotowski, P.
Ciencias Veterinarias
Brahman cattle
generalized glycogenosis
lectin histochemistry
lysosomal storage disorder
title_short Lectin-histochemistry: glycogenosis in cattle
title_full Lectin-histochemistry: glycogenosis in cattle
title_fullStr Lectin-histochemistry: glycogenosis in cattle
title_full_unstemmed Lectin-histochemistry: glycogenosis in cattle
title_sort Lectin-histochemistry: glycogenosis in cattle
dc.creator.none.fl_str_mv Zlotowski, P.
Gimeno, Eduardo Juan
Díaz, A.
Barros, R.
Barros, Severo S.
Farias da Cruz, Claudio Estevao
Driemeier, D.
author Zlotowski, P.
author_facet Zlotowski, P.
Gimeno, Eduardo Juan
Díaz, A.
Barros, R.
Barros, Severo S.
Farias da Cruz, Claudio Estevao
Driemeier, D.
author_role author
author2 Gimeno, Eduardo Juan
Díaz, A.
Barros, R.
Barros, Severo S.
Farias da Cruz, Claudio Estevao
Driemeier, D.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Brahman cattle
generalized glycogenosis
lectin histochemistry
lysosomal storage disorder
topic Ciencias Veterinarias
Brahman cattle
generalized glycogenosis
lectin histochemistry
lysosomal storage disorder
dc.description.none.fl_txt_mv Ten out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis.
Facultad de Ciencias Veterinarias
description Ten out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis.
publishDate 2006
dc.date.none.fl_str_mv 2006
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info:eu-repo/semantics/publishedVersion
Articulo
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dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/145221
url http://sedici.unlp.edu.ar/handle/10915/145221
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0165-7380
info:eu-repo/semantics/altIdentifier/issn/1573-7446
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11259-006-3237-0
info:eu-repo/semantics/altIdentifier/pmid/16502105
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
369-377
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instname:Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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