Lectin-histochemistry: glycogenosis in cattle
- Autores
- Zlotowski, P.; Gimeno, Eduardo Juan; Díaz, A.; Barros, R.; Barros, Severo S.; Farias da Cruz, Claudio Estevao; Driemeier, D.
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Ten out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis.
Facultad de Ciencias Veterinarias - Materia
-
Ciencias Veterinarias
Brahman cattle
generalized glycogenosis
lectin histochemistry
lysosomal storage disorder - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/145221
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Lectin-histochemistry: glycogenosis in cattleZlotowski, P.Gimeno, Eduardo JuanDíaz, A.Barros, R.Barros, Severo S.Farias da Cruz, Claudio EstevaoDriemeier, D.Ciencias VeterinariasBrahman cattlegeneralized glycogenosislectin histochemistrylysosomal storage disorderTen out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis.Facultad de Ciencias Veterinarias2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf369-377http://sedici.unlp.edu.ar/handle/10915/145221enginfo:eu-repo/semantics/altIdentifier/issn/0165-7380info:eu-repo/semantics/altIdentifier/issn/1573-7446info:eu-repo/semantics/altIdentifier/doi/10.1007/s11259-006-3237-0info:eu-repo/semantics/altIdentifier/pmid/16502105info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:21Zoai:sedici.unlp.edu.ar:10915/145221Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:22.072SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Lectin-histochemistry: glycogenosis in cattle |
title |
Lectin-histochemistry: glycogenosis in cattle |
spellingShingle |
Lectin-histochemistry: glycogenosis in cattle Zlotowski, P. Ciencias Veterinarias Brahman cattle generalized glycogenosis lectin histochemistry lysosomal storage disorder |
title_short |
Lectin-histochemistry: glycogenosis in cattle |
title_full |
Lectin-histochemistry: glycogenosis in cattle |
title_fullStr |
Lectin-histochemistry: glycogenosis in cattle |
title_full_unstemmed |
Lectin-histochemistry: glycogenosis in cattle |
title_sort |
Lectin-histochemistry: glycogenosis in cattle |
dc.creator.none.fl_str_mv |
Zlotowski, P. Gimeno, Eduardo Juan Díaz, A. Barros, R. Barros, Severo S. Farias da Cruz, Claudio Estevao Driemeier, D. |
author |
Zlotowski, P. |
author_facet |
Zlotowski, P. Gimeno, Eduardo Juan Díaz, A. Barros, R. Barros, Severo S. Farias da Cruz, Claudio Estevao Driemeier, D. |
author_role |
author |
author2 |
Gimeno, Eduardo Juan Díaz, A. Barros, R. Barros, Severo S. Farias da Cruz, Claudio Estevao Driemeier, D. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Veterinarias Brahman cattle generalized glycogenosis lectin histochemistry lysosomal storage disorder |
topic |
Ciencias Veterinarias Brahman cattle generalized glycogenosis lectin histochemistry lysosomal storage disorder |
dc.description.none.fl_txt_mv |
Ten out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis. Facultad de Ciencias Veterinarias |
description |
Ten out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
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publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/145221 |
url |
http://sedici.unlp.edu.ar/handle/10915/145221 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/0165-7380 info:eu-repo/semantics/altIdentifier/issn/1573-7446 info:eu-repo/semantics/altIdentifier/doi/10.1007/s11259-006-3237-0 info:eu-repo/semantics/altIdentifier/pmid/16502105 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
dc.format.none.fl_str_mv |
application/pdf 369-377 |
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