Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense

Autores
Sant’Ana, Fabiano J.F.; Barbeito, Claudio Gustavo; Nishida, Fabian; Gimeno, Eduardo Juan; Verdes, José M.; Moraña, Antonio; Barros, Claudio S.L.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microscopic and lectin histochemical studies were performed using the cerebella of 33 natural cases of Solanum fastigiatum var. fastigiatum intoxication in the cattle from southern Brazil and 2 natural and 4 experimental cases of Solanum bonariense from Uruguay. The following biotinylated lectins were used in both cases: WGA, sWGA, BS-I, Con-A, RCA-I, DBA, and UEA-I, with the addition of LCA in S. fastigiatum poisoning cases. Histologically, the lesions consisted of fine vacuolization, distention of portions of the Purkinje cells, axonal spheroids measuring 14-50 m in the granular cell layer and adjacent white matter and, proliferation of the Bergmann?s glia. Lectin histochemistry revealed strong reactivity of stored material in Purkinje neurons with the lectins sWGA, Con-A, and LCA in S. fastigiatum cases. A similar pattern was found in S. bonariense cases with a most intense reactions to WGA, and less intense reaction to Con-A, whereas BS-I and RCA-I binding was absent to poor in these neurons in all the cases studied.
Lectin reactivity in Purkinje cells between cases was independent of cell damage (from mild to severe loss of neurons). Both S. fastigiatum and S. bonariense have similar lectin binding,  suggesting a similar pathogenesis. Since comparable binding patterns have been described in animals poisoned with swainsonine-containing plants, perhaps the toxins in these plants contain related glycosidaseinhibiting toxins or inhibit glycoprotein and lysosomal metabolism through some related mechanism. The results of this study showed that in spontaneous poisoning by S. fastigiatum and S. bonariense in the cattle, the pattern of lectin binding is similar to those observed in S. fastigiatum experimental conditions.
Fil: Sant’Ana, Fabiano J.F.. Universidade Federal de Santa Catarina; Brasil
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Nishida, Fabian. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Verdes, José M.. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Moraña, Antonio. Universidad de la República; Uruguay
Fil: Barros, Claudio S.L.. Universidad de la República; Uruguay
Materia
Cattle
Cerebellar degeneration
Lectin histochemistry
Neurotoxicity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100481
Acceder
id CONICETDig_0e3c8187f3e84b52e8e7a0bc72b537a6
oai_identifier_str oai:ri.conicet.gov.ar:11336/100481
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonarienseSant’Ana, Fabiano J.F.Barbeito, Claudio GustavoNishida, FabianGimeno, Eduardo JuanVerdes, José M.Moraña, AntonioBarros, Claudio S.L.CattleCerebellar degenerationLectin histochemistryNeurotoxicityhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Microscopic and lectin histochemical studies were performed using the cerebella of 33 natural cases of Solanum fastigiatum var. fastigiatum intoxication in the cattle from southern Brazil and 2 natural and 4 experimental cases of Solanum bonariense from Uruguay. The following biotinylated lectins were used in both cases: WGA, sWGA, BS-I, Con-A, RCA-I, DBA, and UEA-I, with the addition of LCA in S. fastigiatum poisoning cases. Histologically, the lesions consisted of fine vacuolization, distention of portions of the Purkinje cells, axonal spheroids measuring 14-50 m in the granular cell layer and adjacent white matter and, proliferation of the Bergmann?s glia. Lectin histochemistry revealed strong reactivity of stored material in Purkinje neurons with the lectins sWGA, Con-A, and LCA in S. fastigiatum cases. A similar pattern was found in S. bonariense cases with a most intense reactions to WGA, and less intense reaction to Con-A, whereas BS-I and RCA-I binding was absent to poor in these neurons in all the cases studied.<br />Lectin reactivity in Purkinje cells between cases was independent of cell damage (from mild to severe loss of neurons). Both S. fastigiatum and S. bonariense have similar lectin binding,  suggesting a similar pathogenesis. Since comparable binding patterns have been described in animals poisoned with swainsonine-containing plants, perhaps the toxins in these plants contain related glycosidaseinhibiting toxins or inhibit glycoprotein and lysosomal metabolism through some related mechanism. The results of this study showed that in spontaneous poisoning by S. fastigiatum and S. bonariense in the cattle, the pattern of lectin binding is similar to those observed in S. fastigiatum experimental conditions.Fil: Sant’Ana, Fabiano J.F.. Universidade Federal de Santa Catarina; BrasilFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Nishida, Fabian. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Verdes, José M.. Universidad de la República. Facultad de Ciencias; UruguayFil: Moraña, Antonio. Universidad de la República; UruguayFil: Barros, Claudio S.L.. Universidad de la República; UruguayUnited States Department of Agriculture2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100481Sant’Ana, Fabiano J.F.; Barbeito, Claudio Gustavo; Nishida, Fabian; Gimeno, Eduardo Juan; Verdes, José M.; et al.; Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense; United States Department of Agriculture; International Journal of Poisonous Plant Research; 1; 1; 10-2011; 28-342154-3216CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ars.usda.gov/ARSUserFiles/oc/np/PoisonousPlants/PoisonousPlantResearch.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:43Zoai:ri.conicet.gov.ar:11336/100481instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:44.168CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense
title Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense
spellingShingle Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense
Sant’Ana, Fabiano J.F.
Cattle
Cerebellar degeneration
Lectin histochemistry
Neurotoxicity
title_short Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense
title_full Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense
title_fullStr Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense
title_full_unstemmed Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense
title_sort Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense
dc.creator.none.fl_str_mv Sant’Ana, Fabiano J.F.
Barbeito, Claudio Gustavo
Nishida, Fabian
Gimeno, Eduardo Juan
Verdes, José M.
Moraña, Antonio
Barros, Claudio S.L.
author Sant’Ana, Fabiano J.F.
author_facet Sant’Ana, Fabiano J.F.
Barbeito, Claudio Gustavo
Nishida, Fabian
Gimeno, Eduardo Juan
Verdes, José M.
Moraña, Antonio
Barros, Claudio S.L.
author_role author
author2 Barbeito, Claudio Gustavo
Nishida, Fabian
Gimeno, Eduardo Juan
Verdes, José M.
Moraña, Antonio
Barros, Claudio S.L.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cattle
Cerebellar degeneration
Lectin histochemistry
Neurotoxicity
topic Cattle
Cerebellar degeneration
Lectin histochemistry
Neurotoxicity
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Microscopic and lectin histochemical studies were performed using the cerebella of 33 natural cases of Solanum fastigiatum var. fastigiatum intoxication in the cattle from southern Brazil and 2 natural and 4 experimental cases of Solanum bonariense from Uruguay. The following biotinylated lectins were used in both cases: WGA, sWGA, BS-I, Con-A, RCA-I, DBA, and UEA-I, with the addition of LCA in S. fastigiatum poisoning cases. Histologically, the lesions consisted of fine vacuolization, distention of portions of the Purkinje cells, axonal spheroids measuring 14-50 m in the granular cell layer and adjacent white matter and, proliferation of the Bergmann?s glia. Lectin histochemistry revealed strong reactivity of stored material in Purkinje neurons with the lectins sWGA, Con-A, and LCA in S. fastigiatum cases. A similar pattern was found in S. bonariense cases with a most intense reactions to WGA, and less intense reaction to Con-A, whereas BS-I and RCA-I binding was absent to poor in these neurons in all the cases studied.<br />Lectin reactivity in Purkinje cells between cases was independent of cell damage (from mild to severe loss of neurons). Both S. fastigiatum and S. bonariense have similar lectin binding,  suggesting a similar pathogenesis. Since comparable binding patterns have been described in animals poisoned with swainsonine-containing plants, perhaps the toxins in these plants contain related glycosidaseinhibiting toxins or inhibit glycoprotein and lysosomal metabolism through some related mechanism. The results of this study showed that in spontaneous poisoning by S. fastigiatum and S. bonariense in the cattle, the pattern of lectin binding is similar to those observed in S. fastigiatum experimental conditions.
Fil: Sant’Ana, Fabiano J.F.. Universidade Federal de Santa Catarina; Brasil
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Nishida, Fabian. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Verdes, José M.. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Moraña, Antonio. Universidad de la República; Uruguay
Fil: Barros, Claudio S.L.. Universidad de la República; Uruguay
description Microscopic and lectin histochemical studies were performed using the cerebella of 33 natural cases of Solanum fastigiatum var. fastigiatum intoxication in the cattle from southern Brazil and 2 natural and 4 experimental cases of Solanum bonariense from Uruguay. The following biotinylated lectins were used in both cases: WGA, sWGA, BS-I, Con-A, RCA-I, DBA, and UEA-I, with the addition of LCA in S. fastigiatum poisoning cases. Histologically, the lesions consisted of fine vacuolization, distention of portions of the Purkinje cells, axonal spheroids measuring 14-50 m in the granular cell layer and adjacent white matter and, proliferation of the Bergmann?s glia. Lectin histochemistry revealed strong reactivity of stored material in Purkinje neurons with the lectins sWGA, Con-A, and LCA in S. fastigiatum cases. A similar pattern was found in S. bonariense cases with a most intense reactions to WGA, and less intense reaction to Con-A, whereas BS-I and RCA-I binding was absent to poor in these neurons in all the cases studied.<br />Lectin reactivity in Purkinje cells between cases was independent of cell damage (from mild to severe loss of neurons). Both S. fastigiatum and S. bonariense have similar lectin binding,  suggesting a similar pathogenesis. Since comparable binding patterns have been described in animals poisoned with swainsonine-containing plants, perhaps the toxins in these plants contain related glycosidaseinhibiting toxins or inhibit glycoprotein and lysosomal metabolism through some related mechanism. The results of this study showed that in spontaneous poisoning by S. fastigiatum and S. bonariense in the cattle, the pattern of lectin binding is similar to those observed in S. fastigiatum experimental conditions.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100481
Sant’Ana, Fabiano J.F.; Barbeito, Claudio Gustavo; Nishida, Fabian; Gimeno, Eduardo Juan; Verdes, José M.; et al.; Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense; United States Department of Agriculture; International Journal of Poisonous Plant Research; 1; 1; 10-2011; 28-34
2154-3216
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100481
identifier_str_mv Sant’Ana, Fabiano J.F.; Barbeito, Claudio Gustavo; Nishida, Fabian; Gimeno, Eduardo Juan; Verdes, José M.; et al.; Clinical and pathological aspects and cerebellar lectin binding in cattle poisoned with Solanum fastigiatum var. fastigiatum and Solanum bonariense; United States Department of Agriculture; International Journal of Poisonous Plant Research; 1; 1; 10-2011; 28-34
2154-3216
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ars.usda.gov/ARSUserFiles/oc/np/PoisonousPlants/PoisonousPlantResearch.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv United States Department of Agriculture
publisher.none.fl_str_mv United States Department of Agriculture
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269050027114496
score 13.13397

Publicaciones similares