A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.
- Autores
- Dorsch, Matías A.; de Yaniz, María Guadalupe; Fiorani, Franco; Hecker, Yanina Paola; Odeon, Anselmo Carlos; Morrell, Eleonora Lidia; Campero, Carlos Manuel; Barbeito, Claudio Gustavo; Moore, Prando Dadin
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered a placebo. An indirect fluorescence antibody test (IFAT) was performed on serum samples obtained before and after the inoculation. The cows were killed at 30 and 37 days post inoculation. Samples of placenta were taken for histopathology and lectin histochemistry. Fetal tissues and fluids were collected for histopathology and IFAT, respectively. All infected cows had high antibody titres. All fetuses had characteristic histopathological lesions, including non-suppurative meningoencephalitis, myocarditis, hepatitis and myositis, suggesting N. caninum infection. Only two infected fetuses developed specific antibodies. Mild non-suppurative inflammatory infiltrates were recorded in the placentae. Differences in the lectin-binding pattern were observed between infected animals and controls in the glycocalyx (CON-A and WGA) and apical cytoplasm (RCA-I and CON-A) of the trophoblastic cells; giant trophoblastic cells (CON-A and DBA); glycocalyx (PNA, WGA) and apical cytoplasm (CON-A, WGA, PNA, DBA and RCA-I) of endometrial cells; trophoblast of the interplacentomal region (WGA); endothelium (CON-A, SBA, RCA-1 and WGA); and finally, mesenchyme (CON-A, RCA-1, SBA, PNA and DBA). These findings indicate that there is a distinctive pattern of lectin binding in the placenta of cattle infected with N. caninum. The direct effect of the presence of the protozoa as well as the altered expression of cytokines could explain these changes in the maternofetal interface.
EEA Balcarce
Fil: Dorsch, Matias A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: de Yañiz, María Guadalupe. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Moore, Dadín Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. - Fuente
- Journal of Comparative Pathology 166 : 45-53 (January 2019)
- Materia
-
Ganado Bovino
Lectinas
Neospora Caninum
Placenta
Cattle
Lectins - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/6021
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A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.Dorsch, Matías A.de Yaniz, María GuadalupeFiorani, FrancoHecker, Yanina PaolaOdeon, Anselmo CarlosMorrell, Eleonora LidiaCampero, Carlos ManuelBarbeito, Claudio GustavoMoore, Prando DadinGanado BovinoLectinasNeospora CaninumPlacentaCattleLectinsThe aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered a placebo. An indirect fluorescence antibody test (IFAT) was performed on serum samples obtained before and after the inoculation. The cows were killed at 30 and 37 days post inoculation. Samples of placenta were taken for histopathology and lectin histochemistry. Fetal tissues and fluids were collected for histopathology and IFAT, respectively. All infected cows had high antibody titres. All fetuses had characteristic histopathological lesions, including non-suppurative meningoencephalitis, myocarditis, hepatitis and myositis, suggesting N. caninum infection. Only two infected fetuses developed specific antibodies. Mild non-suppurative inflammatory infiltrates were recorded in the placentae. Differences in the lectin-binding pattern were observed between infected animals and controls in the glycocalyx (CON-A and WGA) and apical cytoplasm (RCA-I and CON-A) of the trophoblastic cells; giant trophoblastic cells (CON-A and DBA); glycocalyx (PNA, WGA) and apical cytoplasm (CON-A, WGA, PNA, DBA and RCA-I) of endometrial cells; trophoblast of the interplacentomal region (WGA); endothelium (CON-A, SBA, RCA-1 and WGA); and finally, mesenchyme (CON-A, RCA-1, SBA, PNA and DBA). These findings indicate that there is a distinctive pattern of lectin binding in the placenta of cattle infected with N. caninum. The direct effect of the presence of the protozoa as well as the altered expression of cytokines could explain these changes in the maternofetal interface.EEA BalcarceFil: Dorsch, Matias A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: de Yañiz, María Guadalupe. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Moore, Dadín Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Elsevier2019-09-30T14:34:34Z2019-09-30T14:34:34Z2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0021997518302342http://hdl.handle.net/20.500.12123/60210021-9975https://doi.org/10.1016/j.jcpa.2018.10.172Journal of Comparative Pathology 166 : 45-53 (January 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:47Zoai:localhost:20.500.12123/6021instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:47.58INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
title |
A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
spellingShingle |
A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. Dorsch, Matías A. Ganado Bovino Lectinas Neospora Caninum Placenta Cattle Lectins |
title_short |
A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
title_full |
A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
title_fullStr |
A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
title_full_unstemmed |
A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
title_sort |
A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum. |
dc.creator.none.fl_str_mv |
Dorsch, Matías A. de Yaniz, María Guadalupe Fiorani, Franco Hecker, Yanina Paola Odeon, Anselmo Carlos Morrell, Eleonora Lidia Campero, Carlos Manuel Barbeito, Claudio Gustavo Moore, Prando Dadin |
author |
Dorsch, Matías A. |
author_facet |
Dorsch, Matías A. de Yaniz, María Guadalupe Fiorani, Franco Hecker, Yanina Paola Odeon, Anselmo Carlos Morrell, Eleonora Lidia Campero, Carlos Manuel Barbeito, Claudio Gustavo Moore, Prando Dadin |
author_role |
author |
author2 |
de Yaniz, María Guadalupe Fiorani, Franco Hecker, Yanina Paola Odeon, Anselmo Carlos Morrell, Eleonora Lidia Campero, Carlos Manuel Barbeito, Claudio Gustavo Moore, Prando Dadin |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Ganado Bovino Lectinas Neospora Caninum Placenta Cattle Lectins |
topic |
Ganado Bovino Lectinas Neospora Caninum Placenta Cattle Lectins |
dc.description.none.fl_txt_mv |
The aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered a placebo. An indirect fluorescence antibody test (IFAT) was performed on serum samples obtained before and after the inoculation. The cows were killed at 30 and 37 days post inoculation. Samples of placenta were taken for histopathology and lectin histochemistry. Fetal tissues and fluids were collected for histopathology and IFAT, respectively. All infected cows had high antibody titres. All fetuses had characteristic histopathological lesions, including non-suppurative meningoencephalitis, myocarditis, hepatitis and myositis, suggesting N. caninum infection. Only two infected fetuses developed specific antibodies. Mild non-suppurative inflammatory infiltrates were recorded in the placentae. Differences in the lectin-binding pattern were observed between infected animals and controls in the glycocalyx (CON-A and WGA) and apical cytoplasm (RCA-I and CON-A) of the trophoblastic cells; giant trophoblastic cells (CON-A and DBA); glycocalyx (PNA, WGA) and apical cytoplasm (CON-A, WGA, PNA, DBA and RCA-I) of endometrial cells; trophoblast of the interplacentomal region (WGA); endothelium (CON-A, SBA, RCA-1 and WGA); and finally, mesenchyme (CON-A, RCA-1, SBA, PNA and DBA). These findings indicate that there is a distinctive pattern of lectin binding in the placenta of cattle infected with N. caninum. The direct effect of the presence of the protozoa as well as the altered expression of cytokines could explain these changes in the maternofetal interface. EEA Balcarce Fil: Dorsch, Matias A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: de Yañiz, María Guadalupe. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Moore, Dadín Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. |
description |
The aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered a placebo. An indirect fluorescence antibody test (IFAT) was performed on serum samples obtained before and after the inoculation. The cows were killed at 30 and 37 days post inoculation. Samples of placenta were taken for histopathology and lectin histochemistry. Fetal tissues and fluids were collected for histopathology and IFAT, respectively. All infected cows had high antibody titres. All fetuses had characteristic histopathological lesions, including non-suppurative meningoencephalitis, myocarditis, hepatitis and myositis, suggesting N. caninum infection. Only two infected fetuses developed specific antibodies. Mild non-suppurative inflammatory infiltrates were recorded in the placentae. Differences in the lectin-binding pattern were observed between infected animals and controls in the glycocalyx (CON-A and WGA) and apical cytoplasm (RCA-I and CON-A) of the trophoblastic cells; giant trophoblastic cells (CON-A and DBA); glycocalyx (PNA, WGA) and apical cytoplasm (CON-A, WGA, PNA, DBA and RCA-I) of endometrial cells; trophoblast of the interplacentomal region (WGA); endothelium (CON-A, SBA, RCA-1 and WGA); and finally, mesenchyme (CON-A, RCA-1, SBA, PNA and DBA). These findings indicate that there is a distinctive pattern of lectin binding in the placenta of cattle infected with N. caninum. The direct effect of the presence of the protozoa as well as the altered expression of cytokines could explain these changes in the maternofetal interface. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-30T14:34:34Z 2019-09-30T14:34:34Z 2019-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S0021997518302342 http://hdl.handle.net/20.500.12123/6021 0021-9975 https://doi.org/10.1016/j.jcpa.2018.10.172 |
url |
https://www.sciencedirect.com/science/article/pii/S0021997518302342 http://hdl.handle.net/20.500.12123/6021 https://doi.org/10.1016/j.jcpa.2018.10.172 |
identifier_str_mv |
0021-9975 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Journal of Comparative Pathology 166 : 45-53 (January 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619138133131264 |
score |
12.559606 |