A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.

Autores
Dorsch, Matías A.; de Yaniz, María Guadalupe; Fiorani, Franco; Hecker, Yanina Paola; Odeon, Anselmo Carlos; Morrell, Eleonora Lidia; Campero, Carlos Manuel; Barbeito, Claudio Gustavo; Moore, Prando Dadin
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered a placebo. An indirect fluorescence antibody test (IFAT) was performed on serum samples obtained before and after the inoculation. The cows were killed at 30 and 37 days post inoculation. Samples of placenta were taken for histopathology and lectin histochemistry. Fetal tissues and fluids were collected for histopathology and IFAT, respectively. All infected cows had high antibody titres. All fetuses had characteristic histopathological lesions, including non-suppurative meningoencephalitis, myocarditis, hepatitis and myositis, suggesting N. caninum infection. Only two infected fetuses developed specific antibodies. Mild non-suppurative inflammatory infiltrates were recorded in the placentae. Differences in the lectin-binding pattern were observed between infected animals and controls in the glycocalyx (CON-A and WGA) and apical cytoplasm (RCA-I and CON-A) of the trophoblastic cells; giant trophoblastic cells (CON-A and DBA); glycocalyx (PNA, WGA) and apical cytoplasm (CON-A, WGA, PNA, DBA and RCA-I) of endometrial cells; trophoblast of the interplacentomal region (WGA); endothelium (CON-A, SBA, RCA-1 and WGA); and finally, mesenchyme (CON-A, RCA-1, SBA, PNA and DBA). These findings indicate that there is a distinctive pattern of lectin binding in the placenta of cattle infected with N. caninum. The direct effect of the presence of the protozoa as well as the altered expression of cytokines could explain these changes in the maternofetal interface.
EEA Balcarce
Fil: Dorsch, Matias A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: de Yañiz, María Guadalupe. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Moore, Dadín Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fuente
Journal of Comparative Pathology 166 : 45-53 (January 2019)
Materia
Ganado Bovino
Lectinas
Neospora Caninum
Placenta
Cattle
Lectins
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6021
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6021
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.Dorsch, Matías A.de Yaniz, María GuadalupeFiorani, FrancoHecker, Yanina PaolaOdeon, Anselmo CarlosMorrell, Eleonora LidiaCampero, Carlos ManuelBarbeito, Claudio GustavoMoore, Prando DadinGanado BovinoLectinasNeospora CaninumPlacentaCattleLectinsThe aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered a placebo. An indirect fluorescence antibody test (IFAT) was performed on serum samples obtained before and after the inoculation. The cows were killed at 30 and 37 days post inoculation. Samples of placenta were taken for histopathology and lectin histochemistry. Fetal tissues and fluids were collected for histopathology and IFAT, respectively. All infected cows had high antibody titres. All fetuses had characteristic histopathological lesions, including non-suppurative meningoencephalitis, myocarditis, hepatitis and myositis, suggesting N. caninum infection. Only two infected fetuses developed specific antibodies. Mild non-suppurative inflammatory infiltrates were recorded in the placentae. Differences in the lectin-binding pattern were observed between infected animals and controls in the glycocalyx (CON-A and WGA) and apical cytoplasm (RCA-I and CON-A) of the trophoblastic cells; giant trophoblastic cells (CON-A and DBA); glycocalyx (PNA, WGA) and apical cytoplasm (CON-A, WGA, PNA, DBA and RCA-I) of endometrial cells; trophoblast of the interplacentomal region (WGA); endothelium (CON-A, SBA, RCA-1 and WGA); and finally, mesenchyme (CON-A, RCA-1, SBA, PNA and DBA). These findings indicate that there is a distinctive pattern of lectin binding in the placenta of cattle infected with N. caninum. The direct effect of the presence of the protozoa as well as the altered expression of cytokines could explain these changes in the maternofetal interface.EEA BalcarceFil: Dorsch, Matias A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: de Yañiz, María Guadalupe. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Moore, Dadín Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Elsevier2019-09-30T14:34:34Z2019-09-30T14:34:34Z2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0021997518302342http://hdl.handle.net/20.500.12123/60210021-9975https://doi.org/10.1016/j.jcpa.2018.10.172Journal of Comparative Pathology 166 : 45-53 (January 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:39Zoai:localhost:20.500.12123/6021instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:39.324INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.
title A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.
spellingShingle A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.
Dorsch, Matías A.
Ganado Bovino
Lectinas
Neospora Caninum
Placenta
Cattle
Lectins
title_short A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.
title_full A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.
title_fullStr A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.
title_full_unstemmed A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.
title_sort A descriptive Study of lectin histochemistry of the placenta in cattle following inoculation of Neospora caninum.
dc.creator.none.fl_str_mv Dorsch, Matías A.
de Yaniz, María Guadalupe
Fiorani, Franco
Hecker, Yanina Paola
Odeon, Anselmo Carlos
Morrell, Eleonora Lidia
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
Moore, Prando Dadin
author Dorsch, Matías A.
author_facet Dorsch, Matías A.
de Yaniz, María Guadalupe
Fiorani, Franco
Hecker, Yanina Paola
Odeon, Anselmo Carlos
Morrell, Eleonora Lidia
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
Moore, Prando Dadin
author_role author
author2 de Yaniz, María Guadalupe
Fiorani, Franco
Hecker, Yanina Paola
Odeon, Anselmo Carlos
Morrell, Eleonora Lidia
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
Moore, Prando Dadin
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Lectinas
Neospora Caninum
Placenta
Cattle
Lectins
topic Ganado Bovino
Lectinas
Neospora Caninum
Placenta
Cattle
Lectins
dc.description.none.fl_txt_mv The aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered a placebo. An indirect fluorescence antibody test (IFAT) was performed on serum samples obtained before and after the inoculation. The cows were killed at 30 and 37 days post inoculation. Samples of placenta were taken for histopathology and lectin histochemistry. Fetal tissues and fluids were collected for histopathology and IFAT, respectively. All infected cows had high antibody titres. All fetuses had characteristic histopathological lesions, including non-suppurative meningoencephalitis, myocarditis, hepatitis and myositis, suggesting N. caninum infection. Only two infected fetuses developed specific antibodies. Mild non-suppurative inflammatory infiltrates were recorded in the placentae. Differences in the lectin-binding pattern were observed between infected animals and controls in the glycocalyx (CON-A and WGA) and apical cytoplasm (RCA-I and CON-A) of the trophoblastic cells; giant trophoblastic cells (CON-A and DBA); glycocalyx (PNA, WGA) and apical cytoplasm (CON-A, WGA, PNA, DBA and RCA-I) of endometrial cells; trophoblast of the interplacentomal region (WGA); endothelium (CON-A, SBA, RCA-1 and WGA); and finally, mesenchyme (CON-A, RCA-1, SBA, PNA and DBA). These findings indicate that there is a distinctive pattern of lectin binding in the placenta of cattle infected with N. caninum. The direct effect of the presence of the protozoa as well as the altered expression of cytokines could explain these changes in the maternofetal interface.
EEA Balcarce
Fil: Dorsch, Matias A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: de Yañiz, María Guadalupe. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Moore, Dadín Prando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
description The aim of this study was to describe the lectin-binding pattern in the placentas of cows infected experimentally with Neospora caninum. Four cows were inoculated intravenously with 1 × 108 tachyzoites of the NC-1 strain of N. caninum at 150 ± 7 days of pregnancy. Two control cows were administered a placebo. An indirect fluorescence antibody test (IFAT) was performed on serum samples obtained before and after the inoculation. The cows were killed at 30 and 37 days post inoculation. Samples of placenta were taken for histopathology and lectin histochemistry. Fetal tissues and fluids were collected for histopathology and IFAT, respectively. All infected cows had high antibody titres. All fetuses had characteristic histopathological lesions, including non-suppurative meningoencephalitis, myocarditis, hepatitis and myositis, suggesting N. caninum infection. Only two infected fetuses developed specific antibodies. Mild non-suppurative inflammatory infiltrates were recorded in the placentae. Differences in the lectin-binding pattern were observed between infected animals and controls in the glycocalyx (CON-A and WGA) and apical cytoplasm (RCA-I and CON-A) of the trophoblastic cells; giant trophoblastic cells (CON-A and DBA); glycocalyx (PNA, WGA) and apical cytoplasm (CON-A, WGA, PNA, DBA and RCA-I) of endometrial cells; trophoblast of the interplacentomal region (WGA); endothelium (CON-A, SBA, RCA-1 and WGA); and finally, mesenchyme (CON-A, RCA-1, SBA, PNA and DBA). These findings indicate that there is a distinctive pattern of lectin binding in the placenta of cattle infected with N. caninum. The direct effect of the presence of the protozoa as well as the altered expression of cytokines could explain these changes in the maternofetal interface.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-30T14:34:34Z
2019-09-30T14:34:34Z
2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0021997518302342
http://hdl.handle.net/20.500.12123/6021
0021-9975
https://doi.org/10.1016/j.jcpa.2018.10.172
url https://www.sciencedirect.com/science/article/pii/S0021997518302342
http://hdl.handle.net/20.500.12123/6021
https://doi.org/10.1016/j.jcpa.2018.10.172
identifier_str_mv 0021-9975
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Comparative Pathology 166 : 45-53 (January 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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