Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR

Autores
Imperiale, Belén Rocío; Moyano, Roberto Damián; Di Giulio, A. B.; Romero, Magalí Andrea; Alvarado Pinedo, María Fiorella; Santangelo, María de la Paz; Travería, Gabriel Eduardo; Morcillo, N. S.; Romano, María Isabel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours.inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.
Facultad de Ciencias Veterinarias
Centro de Diagnóstico e Investigaciones Veterinarias
Materia
Ciencias Veterinarias
Diversity
MIRU-VNTR
Mycobacterium avium complex
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/87667
Acceder
id SEDICI_1833f90c850a80c130953fee0a0bc901
oai_identifier_str oai:sedici.unlp.edu.ar:10915/87667
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTRImperiale, Belén RocíoMoyano, Roberto DamiánDi Giulio, A. B.Romero, Magalí AndreaAlvarado Pinedo, María FiorellaSantangelo, María de la PazTravería, Gabriel EduardoMorcillo, N. S.Romano, María IsabelCiencias VeterinariasDiversityMIRU-VNTRMycobacterium avium complex<i>Mycobacterium avium</i> sp. <i>avium</i> (MAA), <i>M. avium</i> sp. <i>hominissuis</i> (MAH), and <i>M. avium</i> sp. <i>paratuberculosis</i> (MAP) are the main members of the <i>M. avium</i> complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify <i>Mycobacterium</i> species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours.inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.Facultad de Ciencias VeterinariasCentro de Diagnóstico e Investigaciones Veterinarias2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1382-1391http://sedici.unlp.edu.ar/handle/10915/87667enginfo:eu-repo/semantics/altIdentifier/issn/0950-2688info:eu-repo/semantics/altIdentifier/doi/10.1017/S0950268817000139info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:49:31Zoai:sedici.unlp.edu.ar:10915/87667Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:49:31.405SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
title Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
spellingShingle Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
Imperiale, Belén Rocío
Ciencias Veterinarias
Diversity
MIRU-VNTR
Mycobacterium avium complex
title_short Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
title_full Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
title_fullStr Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
title_full_unstemmed Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
title_sort Genetic diversity of <i>Mycobacterium avium</i> complex strains isolated in Argentina by MIRU-VNTR
dc.creator.none.fl_str_mv Imperiale, Belén Rocío
Moyano, Roberto Damián
Di Giulio, A. B.
Romero, Magalí Andrea
Alvarado Pinedo, María Fiorella
Santangelo, María de la Paz
Travería, Gabriel Eduardo
Morcillo, N. S.
Romano, María Isabel
author Imperiale, Belén Rocío
author_facet Imperiale, Belén Rocío
Moyano, Roberto Damián
Di Giulio, A. B.
Romero, Magalí Andrea
Alvarado Pinedo, María Fiorella
Santangelo, María de la Paz
Travería, Gabriel Eduardo
Morcillo, N. S.
Romano, María Isabel
author_role author
author2 Moyano, Roberto Damián
Di Giulio, A. B.
Romero, Magalí Andrea
Alvarado Pinedo, María Fiorella
Santangelo, María de la Paz
Travería, Gabriel Eduardo
Morcillo, N. S.
Romano, María Isabel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Diversity
MIRU-VNTR
Mycobacterium avium complex
topic Ciencias Veterinarias
Diversity
MIRU-VNTR
Mycobacterium avium complex
dc.description.none.fl_txt_mv <i>Mycobacterium avium</i> sp. <i>avium</i> (MAA), <i>M. avium</i> sp. <i>hominissuis</i> (MAH), and <i>M. avium</i> sp. <i>paratuberculosis</i> (MAP) are the main members of the <i>M. avium</i> complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify <i>Mycobacterium</i> species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours.inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.
Facultad de Ciencias Veterinarias
Centro de Diagnóstico e Investigaciones Veterinarias
description <i>Mycobacterium avium</i> sp. <i>avium</i> (MAA), <i>M. avium</i> sp. <i>hominissuis</i> (MAH), and <i>M. avium</i> sp. <i>paratuberculosis</i> (MAP) are the main members of the <i>M. avium</i> complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify <i>Mycobacterium</i> species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours.inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/87667
url http://sedici.unlp.edu.ar/handle/10915/87667
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0950-2688
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0950268817000139
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
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