Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis

Autores
Gioffré, Andrea; Correa Muñoz, Magnolia; Alvarado Pinedo, María Fiorella; Vaca, Roberto José Antonio; Morsella, Claudia; Fiorentino, María Andrea; Paolicchi, Fernando; Ruybal, Paula; Zumárraga, Martín; Travería, Gabriel Eduardo; Romano, María Isabel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Multiple-locus variable number-tandem repeat analysis (MLVA) of Mycobacterium avium subspecies paratuberculosis (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS900-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level. This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
MIRU-VNTR
MLVA
Molecular typing
Paratuberculosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/86053
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/86053
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysisGioffré, AndreaCorrea Muñoz, MagnoliaAlvarado Pinedo, María FiorellaVaca, Roberto José AntonioMorsella, ClaudiaFiorentino, María AndreaPaolicchi, FernandoRuybal, PaulaZumárraga, MartínTravería, Gabriel EduardoRomano, María IsabelCiencias VeterinariasMIRU-VNTRMLVAMolecular typingParatuberculosisMultiple-locus variable number-tandem repeat analysis (MLVA) of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS<i>900</i>-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level. This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents.Facultad de Ciencias Veterinarias2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf557-564http://sedici.unlp.edu.ar/handle/10915/86053enginfo:eu-repo/semantics/altIdentifier/issn/1517-8382info:eu-repo/semantics/altIdentifier/doi/10.1590/S1517-838246220140283info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:08:55Zoai:sedici.unlp.edu.ar:10915/86053Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:08:55.615SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
title Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
spellingShingle Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
Gioffré, Andrea
Ciencias Veterinarias
MIRU-VNTR
MLVA
Molecular typing
Paratuberculosis
title_short Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
title_full Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
title_fullStr Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
title_full_unstemmed Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
title_sort Molecular typing of Argentinian <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> isolates by multiple-locus variable number-tandem repeat analysis
dc.creator.none.fl_str_mv Gioffré, Andrea
Correa Muñoz, Magnolia
Alvarado Pinedo, María Fiorella
Vaca, Roberto José Antonio
Morsella, Claudia
Fiorentino, María Andrea
Paolicchi, Fernando
Ruybal, Paula
Zumárraga, Martín
Travería, Gabriel Eduardo
Romano, María Isabel
author Gioffré, Andrea
author_facet Gioffré, Andrea
Correa Muñoz, Magnolia
Alvarado Pinedo, María Fiorella
Vaca, Roberto José Antonio
Morsella, Claudia
Fiorentino, María Andrea
Paolicchi, Fernando
Ruybal, Paula
Zumárraga, Martín
Travería, Gabriel Eduardo
Romano, María Isabel
author_role author
author2 Correa Muñoz, Magnolia
Alvarado Pinedo, María Fiorella
Vaca, Roberto José Antonio
Morsella, Claudia
Fiorentino, María Andrea
Paolicchi, Fernando
Ruybal, Paula
Zumárraga, Martín
Travería, Gabriel Eduardo
Romano, María Isabel
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
MIRU-VNTR
MLVA
Molecular typing
Paratuberculosis
topic Ciencias Veterinarias
MIRU-VNTR
MLVA
Molecular typing
Paratuberculosis
dc.description.none.fl_txt_mv Multiple-locus variable number-tandem repeat analysis (MLVA) of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS<i>900</i>-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level. This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents.
Facultad de Ciencias Veterinarias
description Multiple-locus variable number-tandem repeat analysis (MLVA) of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> (MAP) isolates may contribute to the knowledge of strain diversity in Argentina. Although the diversity of MAP has been previously investigated in Argentina using IS<i>900</i>-RFLP, a small number of isolates were employed, and a low discriminative power was reached. The aim of the present study was to test the genetic diversity among MAP isolates using an MLVA approach based on 8 repetitive loci. We studied 97 isolates from cattle, goat and sheep and could describe 7 different patterns: INMV1, INMV2, INMV11, INMV13, INMV16, INMV33 and one incomplete pattern. INMV1 and INMV2 were the most frequent patterns, grouping 76.3% of the isolates. We were also able to demonstrate the coexistence of genotypes in herds and co-infection at the organism level. This study shows that all the patterns described are common to those described in Europe, suggesting an epidemiological link between the continents.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/86053
url http://sedici.unlp.edu.ar/handle/10915/86053
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1517-8382
info:eu-repo/semantics/altIdentifier/doi/10.1590/S1517-838246220140283
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
557-564
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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