Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR

Autores
Imperiale, Belen Rocio; Moyano, Roberto Damian; Di Giulio, A.B.; Romero, M.A.; Alvarado Pinedo, Maria Fiorella; Santangelo, María De La Paz; Travería, G.E.; Morcillo, N.S.; Romano, Maria Isabel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unitvariable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours. inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.
Inst. de Biotecnología
Fil: Imperiale, Belen Rocio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giulio, A.B. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; San Fernando, Argentina
Fil: Romero, M.A. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina
Fil: Alvarado Pinedo, Maria Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Travería, G.E. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina
Fil: Morcillo, Nora S.Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Epidemiology and infection 145 (7) : 1382-1391. (2017)
Materia
Genética
Mycobacterium avium
Variación Genética
Genetics
Genetic Variation
Diversidad Genética
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/947
Acceder
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oai_identifier_str oai:localhost:20.500.12123/947
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network_name_str INTA Digital (INTA)
spelling Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTRImperiale, Belen RocioMoyano, Roberto DamianDi Giulio, A.B.Romero, M.A.Alvarado Pinedo, Maria FiorellaSantangelo, María De La PazTravería, G.E.Morcillo, N.S.Romano, Maria IsabelGenéticaMycobacterium aviumVariación GenéticaGeneticsGenetic VariationDiversidad GenéticaMycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unitvariable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours. inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.Inst. de BiotecnologíaFil: Imperiale, Belen Rocio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Di Giulio, A.B. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; San Fernando, ArgentinaFil: Romero, M.A. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; ArgentinaFil: Alvarado Pinedo, Maria Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; ArgentinaFil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Travería, G.E. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; ArgentinaFil: Morcillo, Nora S.Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2017-08-10T15:48:13Z2017-08-10T15:48:13Z2017-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/947https://www.cambridge.org/core/journals/epidemiology-and-infection/article/genetic-diversity-of-mycobacterium-avium-complex-strains-isolated-in-argentina-by-miruvntr/5C0EF5C3EFC75989893FD6B6631E99620950-2688 (Print)1469-4409 (Online)https://doi.org/10.1017/S0950268817000139Epidemiology and infection 145 (7) : 1382-1391. (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/restrictedAccess2025-09-04T09:46:57Zoai:localhost:20.500.12123/947instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:46:58.231INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR
title Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR
spellingShingle Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR
Imperiale, Belen Rocio
Genética
Mycobacterium avium
Variación Genética
Genetics
Genetic Variation
Diversidad Genética
title_short Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR
title_full Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR
title_fullStr Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR
title_full_unstemmed Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR
title_sort Genetic diversity of Mycobacterium avium complex strains isolated in Argentina by MIRU-VNTR
dc.creator.none.fl_str_mv Imperiale, Belen Rocio
Moyano, Roberto Damian
Di Giulio, A.B.
Romero, M.A.
Alvarado Pinedo, Maria Fiorella
Santangelo, María De La Paz
Travería, G.E.
Morcillo, N.S.
Romano, Maria Isabel
author Imperiale, Belen Rocio
author_facet Imperiale, Belen Rocio
Moyano, Roberto Damian
Di Giulio, A.B.
Romero, M.A.
Alvarado Pinedo, Maria Fiorella
Santangelo, María De La Paz
Travería, G.E.
Morcillo, N.S.
Romano, Maria Isabel
author_role author
author2 Moyano, Roberto Damian
Di Giulio, A.B.
Romero, M.A.
Alvarado Pinedo, Maria Fiorella
Santangelo, María De La Paz
Travería, G.E.
Morcillo, N.S.
Romano, Maria Isabel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Genética
Mycobacterium avium
Variación Genética
Genetics
Genetic Variation
Diversidad Genética
topic Genética
Mycobacterium avium
Variación Genética
Genetics
Genetic Variation
Diversidad Genética
dc.description.none.fl_txt_mv Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unitvariable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours. inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.
Inst. de Biotecnología
Fil: Imperiale, Belen Rocio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Di Giulio, A.B. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; San Fernando, Argentina
Fil: Romero, M.A. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina
Fil: Alvarado Pinedo, Maria Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Travería, G.E. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria; Argentina
Fil: Morcillo, Nora S.Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP. Genotyping was performed using a mycobacterial interspersed repetitive-unitvariable-number tandem-repeat (MIRU-VNTR) scheme (loci: 292, X3, 25, 47, 3, 7, 10, 32) and patterns (INMV) were assigned according to the MAC-INMV database (http://mac-inmv.tours. inra.fr/). Twenty-two (22/26, 84·6%) MAH isolates were genotyped and 16 were grouped into the following, INMV 92, INMV 121, INMV 97, INMV 103, INMV 50, and INMV 40. The loci X3 and 25 showed the largest diversity (D: 0·5844), and the global discriminatory index (Hunter and Gaston discriminatory index, HGDI) was 0·9300. MAP (100%) isolates were grouped into INMV 1, INMV 2, INMV 11, INMV 8, and INMV 5. The HGDI was 0·6984 and loci 292 and 7 had the largest D (0·6980 and 0·5050). MAH presented a higher D when compared with MAP. The MIRU-VNTR was a useful tool to describe the genetic diversity of both MAH and MAP as well as to identify six new MAH patterns that were conveniently reported to the MAC-INMV database. It was also demonstrated that, in the geographical region studied, human MAC cases were produced by MAH as there was no MAA found among the human clinical samples.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-10T15:48:13Z
2017-08-10T15:48:13Z
2017-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/947
https://www.cambridge.org/core/journals/epidemiology-and-infection/article/genetic-diversity-of-mycobacterium-avium-complex-strains-isolated-in-argentina-by-miruvntr/5C0EF5C3EFC75989893FD6B6631E9962
0950-2688 (Print)
1469-4409 (Online)
https://doi.org/10.1017/S0950268817000139
url http://hdl.handle.net/20.500.12123/947
https://www.cambridge.org/core/journals/epidemiology-and-infection/article/genetic-diversity-of-mycobacterium-avium-complex-strains-isolated-in-argentina-by-miruvntr/5C0EF5C3EFC75989893FD6B6631E9962
https://doi.org/10.1017/S0950268817000139
identifier_str_mv 0950-2688 (Print)
1469-4409 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
dc.source.none.fl_str_mv Epidemiology and infection 145 (7) : 1382-1391. (2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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