Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis

Autores
García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Cecilia; Sota, Rodolfo Luzbel de la; Stornelli, María Alejandra
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Endometritis is a major cause of infertility in many domestic species. However, until now the pathogenesis of the endometritis in the bitch is unclear. The aim of this study was to evaluate the gene transcription pattern of prostaglandin (PG) synthesis enzymes (cyclooxygenase [COX2], PTGES-1 and PGFS) in the endometrium of bitches with or without endometritis. Thirty mixed breed bitches in dioestrus, aged between 1 and 5 years, and weighing between 10 and 30 kg were used. After ovariohysterectomy (OVX), uterine biopsy samples were collected from the middle part of both horns. Then, endometrial epithelium was collected using the cytobrush method and mRNA analysis was performed by real-time RT-PCR. Data were analysed with Kruskal–Wallis anova using the sas® software. Uterine condition was identified by endometrial biopsies (normal endometria [n = 11; NE], acute endometritis [n = 10; AE] and chronic endometritis [n = 9; CE]). The COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in bitches with and without endometritis was similar. Except for PGFS/AKR1C3, gene transcription of COX2 and PTGES-1 was significantly increased in AE compared with CE. In addition, COX2 gene transcription was significantly increased in AE compared with NE. In contrast, no differences were found for COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in the samples of NE compared with CE.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Endometritis
Domestic species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/87656

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network_name_str SEDICI (UNLP)
spelling Prostaglandin synthesis enzymes' gene transcription in bitches with endometritisGarcía Mitacek, María CarlaPraderio, Romina GiseleStornelli, María CeciliaSota, Rodolfo Luzbel de laStornelli, María AlejandraCiencias VeterinariasEndometritisDomestic speciesEndometritis is a major cause of infertility in many domestic species. However, until now the pathogenesis of the endometritis in the bitch is unclear. The aim of this study was to evaluate the gene transcription pattern of prostaglandin (PG) synthesis enzymes (cyclooxygenase [COX2], PTGES-1 and PGFS) in the endometrium of bitches with or without endometritis. Thirty mixed breed bitches in dioestrus, aged between 1 and 5 years, and weighing between 10 and 30 kg were used. After ovariohysterectomy (OVX), uterine biopsy samples were collected from the middle part of both horns. Then, endometrial epithelium was collected using the cytobrush method and mRNA analysis was performed by real-time RT-PCR. Data were analysed with Kruskal–Wallis anova using the sas® software. Uterine condition was identified by endometrial biopsies (normal endometria [n = 11; NE], acute endometritis [n = 10; AE] and chronic endometritis [n = 9; CE]). The COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in bitches with and without endometritis was similar. Except for PGFS/AKR1C3, gene transcription of COX2 and PTGES-1 was significantly increased in AE compared with CE. In addition, COX2 gene transcription was significantly increased in AE compared with NE. In contrast, no differences were found for COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in the samples of NE compared with CE.Facultad de Ciencias Veterinarias2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf298-302http://sedici.unlp.edu.ar/handle/10915/87656enginfo:eu-repo/semantics/altIdentifier/issn/0936-6768info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12950info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:17:14Zoai:sedici.unlp.edu.ar:10915/87656Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:17:15.126SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
title Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
spellingShingle Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
García Mitacek, María Carla
Ciencias Veterinarias
Endometritis
Domestic species
title_short Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
title_full Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
title_fullStr Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
title_full_unstemmed Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
title_sort Prostaglandin synthesis enzymes' gene transcription in bitches with endometritis
dc.creator.none.fl_str_mv García Mitacek, María Carla
Praderio, Romina Gisele
Stornelli, María Cecilia
Sota, Rodolfo Luzbel de la
Stornelli, María Alejandra
author García Mitacek, María Carla
author_facet García Mitacek, María Carla
Praderio, Romina Gisele
Stornelli, María Cecilia
Sota, Rodolfo Luzbel de la
Stornelli, María Alejandra
author_role author
author2 Praderio, Romina Gisele
Stornelli, María Cecilia
Sota, Rodolfo Luzbel de la
Stornelli, María Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Endometritis
Domestic species
topic Ciencias Veterinarias
Endometritis
Domestic species
dc.description.none.fl_txt_mv Endometritis is a major cause of infertility in many domestic species. However, until now the pathogenesis of the endometritis in the bitch is unclear. The aim of this study was to evaluate the gene transcription pattern of prostaglandin (PG) synthesis enzymes (cyclooxygenase [COX2], PTGES-1 and PGFS) in the endometrium of bitches with or without endometritis. Thirty mixed breed bitches in dioestrus, aged between 1 and 5 years, and weighing between 10 and 30 kg were used. After ovariohysterectomy (OVX), uterine biopsy samples were collected from the middle part of both horns. Then, endometrial epithelium was collected using the cytobrush method and mRNA analysis was performed by real-time RT-PCR. Data were analysed with Kruskal–Wallis anova using the sas® software. Uterine condition was identified by endometrial biopsies (normal endometria [n = 11; NE], acute endometritis [n = 10; AE] and chronic endometritis [n = 9; CE]). The COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in bitches with and without endometritis was similar. Except for PGFS/AKR1C3, gene transcription of COX2 and PTGES-1 was significantly increased in AE compared with CE. In addition, COX2 gene transcription was significantly increased in AE compared with NE. In contrast, no differences were found for COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in the samples of NE compared with CE.
Facultad de Ciencias Veterinarias
description Endometritis is a major cause of infertility in many domestic species. However, until now the pathogenesis of the endometritis in the bitch is unclear. The aim of this study was to evaluate the gene transcription pattern of prostaglandin (PG) synthesis enzymes (cyclooxygenase [COX2], PTGES-1 and PGFS) in the endometrium of bitches with or without endometritis. Thirty mixed breed bitches in dioestrus, aged between 1 and 5 years, and weighing between 10 and 30 kg were used. After ovariohysterectomy (OVX), uterine biopsy samples were collected from the middle part of both horns. Then, endometrial epithelium was collected using the cytobrush method and mRNA analysis was performed by real-time RT-PCR. Data were analysed with Kruskal–Wallis anova using the sas® software. Uterine condition was identified by endometrial biopsies (normal endometria [n = 11; NE], acute endometritis [n = 10; AE] and chronic endometritis [n = 9; CE]). The COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in bitches with and without endometritis was similar. Except for PGFS/AKR1C3, gene transcription of COX2 and PTGES-1 was significantly increased in AE compared with CE. In addition, COX2 gene transcription was significantly increased in AE compared with NE. In contrast, no differences were found for COX2, PTGES-1 and PGFS/AKR1C3 mRNA expression in the samples of NE compared with CE.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/87656
url http://sedici.unlp.edu.ar/handle/10915/87656
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0936-6768
info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12950
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
298-302
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instname:Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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