Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis

Autores
García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Cecilia; de la Sota, Rodolfo Luzbel; Stornelli, María Alejandra
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Our study aimed to evaluate the gene transcription of Cyclooxygenase 1 (COX1), cytokines (Interleukin 6 [IL6], Interleukin 8 [IL8], and Leukemia inhibitory factor [LIF]), and Progesterone receptor (PR) in clinically healthy bitches with normal endometrium and with endometritis without the presence of cystic endometrial hyperplasia. Forty-eight mixed breed bitches in dioestrus were used. Uterine biopsies were collected for diagnosis (normal endometrium [n = 15], and endometritis [n = 30]). Three samples were excluded from the study (two bitches with cystic endometrial hyperplasia and one bitch with atrophy). Samples were collected by cytobrush to quantify mRNA by qPCR. Data were analyzed using a generalized mixed model. COX1, IL6, and IL8 mRNA expression in bitches with endometritis was significantly higher than in normal endometrium (p=0.0002, p=0.002, and p=0.003, respectively). In contrast, LIF and PR mRNA expression in bitches with endometritis was significantly lower than in normal endometrium (p=0.006 and p=0.001, respectively). IL6 (AUC 0.67) was the most favorable biomolecular marker for predicting endometritis in bitches. There was an evident change in the mRNA expression of COX1, cytokines, and PR in clinically healthy bitches with normal endometrium and endometritis without cystic endometrial hyperplasia. These results suggest that biomolecular markers such as COX1, IL6, IL8, LIF, and PR could help diagnose endometritis in the bitch.
Fil: García Mitacek, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Praderio, Romina Gisele. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Stornelli, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina
Fil: de la Sota, Rodolfo Luzbel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Stornelli, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina
Materia
BITCH
ENDOMETRITIS
CYCLOOXYGENASE 1
CYTOKINES
PROGESTERONE RECEPTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232440
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/232440
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritisGarcía Mitacek, María CarlaPraderio, Romina GiseleStornelli, María Ceciliade la Sota, Rodolfo LuzbelStornelli, María AlejandraBITCHENDOMETRITISCYCLOOXYGENASE 1CYTOKINESPROGESTERONE RECEPTORhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Our study aimed to evaluate the gene transcription of Cyclooxygenase 1 (COX1), cytokines (Interleukin 6 [IL6], Interleukin 8 [IL8], and Leukemia inhibitory factor [LIF]), and Progesterone receptor (PR) in clinically healthy bitches with normal endometrium and with endometritis without the presence of cystic endometrial hyperplasia. Forty-eight mixed breed bitches in dioestrus were used. Uterine biopsies were collected for diagnosis (normal endometrium [n = 15], and endometritis [n = 30]). Three samples were excluded from the study (two bitches with cystic endometrial hyperplasia and one bitch with atrophy). Samples were collected by cytobrush to quantify mRNA by qPCR. Data were analyzed using a generalized mixed model. COX1, IL6, and IL8 mRNA expression in bitches with endometritis was significantly higher than in normal endometrium (p=0.0002, p=0.002, and p=0.003, respectively). In contrast, LIF and PR mRNA expression in bitches with endometritis was significantly lower than in normal endometrium (p=0.006 and p=0.001, respectively). IL6 (AUC 0.67) was the most favorable biomolecular marker for predicting endometritis in bitches. There was an evident change in the mRNA expression of COX1, cytokines, and PR in clinically healthy bitches with normal endometrium and endometritis without cystic endometrial hyperplasia. These results suggest that biomolecular markers such as COX1, IL6, IL8, LIF, and PR could help diagnose endometritis in the bitch.Fil: García Mitacek, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Praderio, Romina Gisele. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Stornelli, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; ArgentinaFil: de la Sota, Rodolfo Luzbel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Stornelli, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; ArgentinaUniv Zagreb Vet Faculty2023-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232440García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Cecilia; de la Sota, Rodolfo Luzbel; Stornelli, María Alejandra; Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis; Univ Zagreb Vet Faculty; Veterinarski Arhiv; 93; 6-2023; 683-6940372-5480CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/ 10.24099/vet.arhiv.1968info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:18Zoai:ri.conicet.gov.ar:11336/232440instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:19.091CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis
title Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis
spellingShingle Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis
García Mitacek, María Carla
BITCH
ENDOMETRITIS
CYCLOOXYGENASE 1
CYTOKINES
PROGESTERONE RECEPTOR
title_short Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis
title_full Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis
title_fullStr Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis
title_full_unstemmed Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis
title_sort Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis
dc.creator.none.fl_str_mv García Mitacek, María Carla
Praderio, Romina Gisele
Stornelli, María Cecilia
de la Sota, Rodolfo Luzbel
Stornelli, María Alejandra
author García Mitacek, María Carla
author_facet García Mitacek, María Carla
Praderio, Romina Gisele
Stornelli, María Cecilia
de la Sota, Rodolfo Luzbel
Stornelli, María Alejandra
author_role author
author2 Praderio, Romina Gisele
Stornelli, María Cecilia
de la Sota, Rodolfo Luzbel
Stornelli, María Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv BITCH
ENDOMETRITIS
CYCLOOXYGENASE 1
CYTOKINES
PROGESTERONE RECEPTOR
topic BITCH
ENDOMETRITIS
CYCLOOXYGENASE 1
CYTOKINES
PROGESTERONE RECEPTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Our study aimed to evaluate the gene transcription of Cyclooxygenase 1 (COX1), cytokines (Interleukin 6 [IL6], Interleukin 8 [IL8], and Leukemia inhibitory factor [LIF]), and Progesterone receptor (PR) in clinically healthy bitches with normal endometrium and with endometritis without the presence of cystic endometrial hyperplasia. Forty-eight mixed breed bitches in dioestrus were used. Uterine biopsies were collected for diagnosis (normal endometrium [n = 15], and endometritis [n = 30]). Three samples were excluded from the study (two bitches with cystic endometrial hyperplasia and one bitch with atrophy). Samples were collected by cytobrush to quantify mRNA by qPCR. Data were analyzed using a generalized mixed model. COX1, IL6, and IL8 mRNA expression in bitches with endometritis was significantly higher than in normal endometrium (p=0.0002, p=0.002, and p=0.003, respectively). In contrast, LIF and PR mRNA expression in bitches with endometritis was significantly lower than in normal endometrium (p=0.006 and p=0.001, respectively). IL6 (AUC 0.67) was the most favorable biomolecular marker for predicting endometritis in bitches. There was an evident change in the mRNA expression of COX1, cytokines, and PR in clinically healthy bitches with normal endometrium and endometritis without cystic endometrial hyperplasia. These results suggest that biomolecular markers such as COX1, IL6, IL8, LIF, and PR could help diagnose endometritis in the bitch.
Fil: García Mitacek, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Praderio, Romina Gisele. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Stornelli, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina
Fil: de la Sota, Rodolfo Luzbel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Stornelli, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina
description Our study aimed to evaluate the gene transcription of Cyclooxygenase 1 (COX1), cytokines (Interleukin 6 [IL6], Interleukin 8 [IL8], and Leukemia inhibitory factor [LIF]), and Progesterone receptor (PR) in clinically healthy bitches with normal endometrium and with endometritis without the presence of cystic endometrial hyperplasia. Forty-eight mixed breed bitches in dioestrus were used. Uterine biopsies were collected for diagnosis (normal endometrium [n = 15], and endometritis [n = 30]). Three samples were excluded from the study (two bitches with cystic endometrial hyperplasia and one bitch with atrophy). Samples were collected by cytobrush to quantify mRNA by qPCR. Data were analyzed using a generalized mixed model. COX1, IL6, and IL8 mRNA expression in bitches with endometritis was significantly higher than in normal endometrium (p=0.0002, p=0.002, and p=0.003, respectively). In contrast, LIF and PR mRNA expression in bitches with endometritis was significantly lower than in normal endometrium (p=0.006 and p=0.001, respectively). IL6 (AUC 0.67) was the most favorable biomolecular marker for predicting endometritis in bitches. There was an evident change in the mRNA expression of COX1, cytokines, and PR in clinically healthy bitches with normal endometrium and endometritis without cystic endometrial hyperplasia. These results suggest that biomolecular markers such as COX1, IL6, IL8, LIF, and PR could help diagnose endometritis in the bitch.
publishDate 2023
dc.date.none.fl_str_mv 2023-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232440
García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Cecilia; de la Sota, Rodolfo Luzbel; Stornelli, María Alejandra; Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis; Univ Zagreb Vet Faculty; Veterinarski Arhiv; 93; 6-2023; 683-694
0372-5480
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232440
identifier_str_mv García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Cecilia; de la Sota, Rodolfo Luzbel; Stornelli, María Alejandra; Endometrial mRNA expression of key inflammatory genes and progesterone receptor in uterine tissue of bitches with endometritis; Univ Zagreb Vet Faculty; Veterinarski Arhiv; 93; 6-2023; 683-694
0372-5480
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/ 10.24099/vet.arhiv.1968
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Univ Zagreb Vet Faculty
publisher.none.fl_str_mv Univ Zagreb Vet Faculty
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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