Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2

Autores
García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Alejandra; de la Sota, Rodolfo Luzbel; Stornelli, María Cecilia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known. Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bitches with normal uterine tissue and bitches with endometritis. Methods: Forty-eight mixed breed bitches in diestrus were used. Uterine biopsies were collected for diagnosis [normal endometrium (n = 15; NE), cystic endometrial hyperplasia (n = 1), atrophy (n= 2), acute endometritis (n = 9; AE), subacute endometritis (n = 7; SE), and chronic endometritis (n = 14; CE)]. Immunostaining and quantification of positively stained cells was performed on full-thickness uterine biopsies. Data were analyzed by the GLIMMIX procedure of SAS. Results: COX2 immunostaining was scattered and restricted to cells in the stroma in bitches with NE. However, in bitches with endometritis, strong staining was observed in the luminal epithelium, glandular epithelium, and stromal cells. Staining was also observed in inflammatory cells localized in the stroma as well as inside of the glands. The percentage of COX2 positive stromal cells in bitches with AE, SE, and CE was significantly higher compared with NE (p < 0.005). In addition, the percentage of COX2 positive stromal cells in bitches with SE, and CE was significantly lower compared with AE (p < 0.003). Conclusion: COX2 could be involved in the pathophysiological mechanisms producing endometritis without the presence of cystic endometrial hyperplasia in bitches. However, further researches on this topic are required.
Fil: García Mitacek, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Praderio, Romina Gisele. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Stornelli, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina
Fil: de la Sota, Rodolfo Luzbel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Stornelli, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina
Materia
BITCH
COX2
ENDOMETRITIS
IMMUNOHISTOCHEMICAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/136471
Acceder
id CONICETDig_7b49b950a34f48f1fdf5d6a187b80be6
oai_identifier_str oai:ri.conicet.gov.ar:11336/136471
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2García Mitacek, María CarlaPraderio, Romina GiseleStornelli, María Alejandrade la Sota, Rodolfo LuzbelStornelli, María CeciliaBITCHCOX2ENDOMETRITISIMMUNOHISTOCHEMICALhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known. Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bitches with normal uterine tissue and bitches with endometritis. Methods: Forty-eight mixed breed bitches in diestrus were used. Uterine biopsies were collected for diagnosis [normal endometrium (n = 15; NE), cystic endometrial hyperplasia (n = 1), atrophy (n= 2), acute endometritis (n = 9; AE), subacute endometritis (n = 7; SE), and chronic endometritis (n = 14; CE)]. Immunostaining and quantification of positively stained cells was performed on full-thickness uterine biopsies. Data were analyzed by the GLIMMIX procedure of SAS. Results: COX2 immunostaining was scattered and restricted to cells in the stroma in bitches with NE. However, in bitches with endometritis, strong staining was observed in the luminal epithelium, glandular epithelium, and stromal cells. Staining was also observed in inflammatory cells localized in the stroma as well as inside of the glands. The percentage of COX2 positive stromal cells in bitches with AE, SE, and CE was significantly higher compared with NE (p < 0.005). In addition, the percentage of COX2 positive stromal cells in bitches with SE, and CE was significantly lower compared with AE (p < 0.003). Conclusion: COX2 could be involved in the pathophysiological mechanisms producing endometritis without the presence of cystic endometrial hyperplasia in bitches. However, further researches on this topic are required.Fil: García Mitacek, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Praderio, Romina Gisele. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Stornelli, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; ArgentinaFil: de la Sota, Rodolfo Luzbel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Stornelli, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; ArgentinaUniversity of Tripoli. Faculty of Veterinary Medicine2020-08-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/136471García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Alejandra; de la Sota, Rodolfo Luzbel; Stornelli, María Cecilia; Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 10; 2; 14-8-2020; 157-1632226-44852218-6050CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v10i2.5info:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/198671info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:18:02Zoai:ri.conicet.gov.ar:11336/136471instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:18:02.726CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2
title Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2
spellingShingle Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2
García Mitacek, María Carla
BITCH
COX2
ENDOMETRITIS
IMMUNOHISTOCHEMICAL
title_short Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2
title_full Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2
title_fullStr Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2
title_full_unstemmed Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2
title_sort Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2
dc.creator.none.fl_str_mv García Mitacek, María Carla
Praderio, Romina Gisele
Stornelli, María Alejandra
de la Sota, Rodolfo Luzbel
Stornelli, María Cecilia
author García Mitacek, María Carla
author_facet García Mitacek, María Carla
Praderio, Romina Gisele
Stornelli, María Alejandra
de la Sota, Rodolfo Luzbel
Stornelli, María Cecilia
author_role author
author2 Praderio, Romina Gisele
Stornelli, María Alejandra
de la Sota, Rodolfo Luzbel
Stornelli, María Cecilia
author2_role author
author
author
author
dc.subject.none.fl_str_mv BITCH
COX2
ENDOMETRITIS
IMMUNOHISTOCHEMICAL
topic BITCH
COX2
ENDOMETRITIS
IMMUNOHISTOCHEMICAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known. Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bitches with normal uterine tissue and bitches with endometritis. Methods: Forty-eight mixed breed bitches in diestrus were used. Uterine biopsies were collected for diagnosis [normal endometrium (n = 15; NE), cystic endometrial hyperplasia (n = 1), atrophy (n= 2), acute endometritis (n = 9; AE), subacute endometritis (n = 7; SE), and chronic endometritis (n = 14; CE)]. Immunostaining and quantification of positively stained cells was performed on full-thickness uterine biopsies. Data were analyzed by the GLIMMIX procedure of SAS. Results: COX2 immunostaining was scattered and restricted to cells in the stroma in bitches with NE. However, in bitches with endometritis, strong staining was observed in the luminal epithelium, glandular epithelium, and stromal cells. Staining was also observed in inflammatory cells localized in the stroma as well as inside of the glands. The percentage of COX2 positive stromal cells in bitches with AE, SE, and CE was significantly higher compared with NE (p < 0.005). In addition, the percentage of COX2 positive stromal cells in bitches with SE, and CE was significantly lower compared with AE (p < 0.003). Conclusion: COX2 could be involved in the pathophysiological mechanisms producing endometritis without the presence of cystic endometrial hyperplasia in bitches. However, further researches on this topic are required.
Fil: García Mitacek, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Praderio, Romina Gisele. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Stornelli, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina
Fil: de la Sota, Rodolfo Luzbel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Stornelli, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina
description Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known. Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bitches with normal uterine tissue and bitches with endometritis. Methods: Forty-eight mixed breed bitches in diestrus were used. Uterine biopsies were collected for diagnosis [normal endometrium (n = 15; NE), cystic endometrial hyperplasia (n = 1), atrophy (n= 2), acute endometritis (n = 9; AE), subacute endometritis (n = 7; SE), and chronic endometritis (n = 14; CE)]. Immunostaining and quantification of positively stained cells was performed on full-thickness uterine biopsies. Data were analyzed by the GLIMMIX procedure of SAS. Results: COX2 immunostaining was scattered and restricted to cells in the stroma in bitches with NE. However, in bitches with endometritis, strong staining was observed in the luminal epithelium, glandular epithelium, and stromal cells. Staining was also observed in inflammatory cells localized in the stroma as well as inside of the glands. The percentage of COX2 positive stromal cells in bitches with AE, SE, and CE was significantly higher compared with NE (p < 0.005). In addition, the percentage of COX2 positive stromal cells in bitches with SE, and CE was significantly lower compared with AE (p < 0.003). Conclusion: COX2 could be involved in the pathophysiological mechanisms producing endometritis without the presence of cystic endometrial hyperplasia in bitches. However, further researches on this topic are required.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/136471
García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Alejandra; de la Sota, Rodolfo Luzbel; Stornelli, María Cecilia; Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 10; 2; 14-8-2020; 157-163
2226-4485
2218-6050
CONICET Digital
CONICET
url http://hdl.handle.net/11336/136471
identifier_str_mv García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Alejandra; de la Sota, Rodolfo Luzbel; Stornelli, María Cecilia; Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 10; 2; 14-8-2020; 157-163
2226-4485
2218-6050
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v10i2.5
info:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/198671
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv University of Tripoli. Faculty of Veterinary Medicine
publisher.none.fl_str_mv University of Tripoli. Faculty of Veterinary Medicine
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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