Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2
- Autores
- García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Alejandra; de la Sota, Rodolfo Luzbel; Stornelli, María Cecilia
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known. Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bitches with normal uterine tissue and bitches with endometritis. Methods: Forty-eight mixed breed bitches in diestrus were used. Uterine biopsies were collected for diagnosis [normal endometrium (n = 15; NE), cystic endometrial hyperplasia (n = 1), atrophy (n= 2), acute endometritis (n = 9; AE), subacute endometritis (n = 7; SE), and chronic endometritis (n = 14; CE)]. Immunostaining and quantification of positively stained cells was performed on full-thickness uterine biopsies. Data were analyzed by the GLIMMIX procedure of SAS. Results: COX2 immunostaining was scattered and restricted to cells in the stroma in bitches with NE. However, in bitches with endometritis, strong staining was observed in the luminal epithelium, glandular epithelium, and stromal cells. Staining was also observed in inflammatory cells localized in the stroma as well as inside of the glands. The percentage of COX2 positive stromal cells in bitches with AE, SE, and CE was significantly higher compared with NE (p < 0.005). In addition, the percentage of COX2 positive stromal cells in bitches with SE, and CE was significantly lower compared with AE (p < 0.003). Conclusion: COX2 could be involved in the pathophysiological mechanisms producing endometritis without the presence of cystic endometrial hyperplasia in bitches. However, further researches on this topic are required.
Fil: García Mitacek, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Praderio, Romina Gisele. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Stornelli, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina
Fil: de la Sota, Rodolfo Luzbel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Stornelli, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina - Materia
-
BITCH
COX2
ENDOMETRITIS
IMMUNOHISTOCHEMICAL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/136471
Ver los metadatos del registro completo
id |
CONICETDig_7b49b950a34f48f1fdf5d6a187b80be6 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/136471 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2García Mitacek, María CarlaPraderio, Romina GiseleStornelli, María Alejandrade la Sota, Rodolfo LuzbelStornelli, María CeciliaBITCHCOX2ENDOMETRITISIMMUNOHISTOCHEMICALhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known. Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bitches with normal uterine tissue and bitches with endometritis. Methods: Forty-eight mixed breed bitches in diestrus were used. Uterine biopsies were collected for diagnosis [normal endometrium (n = 15; NE), cystic endometrial hyperplasia (n = 1), atrophy (n= 2), acute endometritis (n = 9; AE), subacute endometritis (n = 7; SE), and chronic endometritis (n = 14; CE)]. Immunostaining and quantification of positively stained cells was performed on full-thickness uterine biopsies. Data were analyzed by the GLIMMIX procedure of SAS. Results: COX2 immunostaining was scattered and restricted to cells in the stroma in bitches with NE. However, in bitches with endometritis, strong staining was observed in the luminal epithelium, glandular epithelium, and stromal cells. Staining was also observed in inflammatory cells localized in the stroma as well as inside of the glands. The percentage of COX2 positive stromal cells in bitches with AE, SE, and CE was significantly higher compared with NE (p < 0.005). In addition, the percentage of COX2 positive stromal cells in bitches with SE, and CE was significantly lower compared with AE (p < 0.003). Conclusion: COX2 could be involved in the pathophysiological mechanisms producing endometritis without the presence of cystic endometrial hyperplasia in bitches. However, further researches on this topic are required.Fil: García Mitacek, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Praderio, Romina Gisele. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Stornelli, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; ArgentinaFil: de la Sota, Rodolfo Luzbel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Stornelli, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; ArgentinaUniversity of Tripoli. Faculty of Veterinary Medicine2020-08-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/136471García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Alejandra; de la Sota, Rodolfo Luzbel; Stornelli, María Cecilia; Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 10; 2; 14-8-2020; 157-1632226-44852218-6050CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v10i2.5info:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/198671info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:46Zoai:ri.conicet.gov.ar:11336/136471instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:47.245CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2 |
title |
Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2 |
spellingShingle |
Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2 García Mitacek, María Carla BITCH COX2 ENDOMETRITIS IMMUNOHISTOCHEMICAL |
title_short |
Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2 |
title_full |
Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2 |
title_fullStr |
Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2 |
title_full_unstemmed |
Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2 |
title_sort |
Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2 |
dc.creator.none.fl_str_mv |
García Mitacek, María Carla Praderio, Romina Gisele Stornelli, María Alejandra de la Sota, Rodolfo Luzbel Stornelli, María Cecilia |
author |
García Mitacek, María Carla |
author_facet |
García Mitacek, María Carla Praderio, Romina Gisele Stornelli, María Alejandra de la Sota, Rodolfo Luzbel Stornelli, María Cecilia |
author_role |
author |
author2 |
Praderio, Romina Gisele Stornelli, María Alejandra de la Sota, Rodolfo Luzbel Stornelli, María Cecilia |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
BITCH COX2 ENDOMETRITIS IMMUNOHISTOCHEMICAL |
topic |
BITCH COX2 ENDOMETRITIS IMMUNOHISTOCHEMICAL |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known. Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bitches with normal uterine tissue and bitches with endometritis. Methods: Forty-eight mixed breed bitches in diestrus were used. Uterine biopsies were collected for diagnosis [normal endometrium (n = 15; NE), cystic endometrial hyperplasia (n = 1), atrophy (n= 2), acute endometritis (n = 9; AE), subacute endometritis (n = 7; SE), and chronic endometritis (n = 14; CE)]. Immunostaining and quantification of positively stained cells was performed on full-thickness uterine biopsies. Data were analyzed by the GLIMMIX procedure of SAS. Results: COX2 immunostaining was scattered and restricted to cells in the stroma in bitches with NE. However, in bitches with endometritis, strong staining was observed in the luminal epithelium, glandular epithelium, and stromal cells. Staining was also observed in inflammatory cells localized in the stroma as well as inside of the glands. The percentage of COX2 positive stromal cells in bitches with AE, SE, and CE was significantly higher compared with NE (p < 0.005). In addition, the percentage of COX2 positive stromal cells in bitches with SE, and CE was significantly lower compared with AE (p < 0.003). Conclusion: COX2 could be involved in the pathophysiological mechanisms producing endometritis without the presence of cystic endometrial hyperplasia in bitches. However, further researches on this topic are required. Fil: García Mitacek, María Carla. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Praderio, Romina Gisele. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Stornelli, María Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina Fil: de la Sota, Rodolfo Luzbel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Stornelli, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Teriogenología. Cátedra de Reproducción Animal; Argentina |
description |
Background: In several mammals, subfertility or infertility associated with endometritis was reported. Although there have been studies about endometritis in bitches, the pathophysiological mechanisms are not completely known. Aim: This study aimed to evaluate the immunohistochemical expression of Cyclooxygenase 2 (COX2) in clinically healthy bitches with normal uterine tissue and bitches with endometritis. Methods: Forty-eight mixed breed bitches in diestrus were used. Uterine biopsies were collected for diagnosis [normal endometrium (n = 15; NE), cystic endometrial hyperplasia (n = 1), atrophy (n= 2), acute endometritis (n = 9; AE), subacute endometritis (n = 7; SE), and chronic endometritis (n = 14; CE)]. Immunostaining and quantification of positively stained cells was performed on full-thickness uterine biopsies. Data were analyzed by the GLIMMIX procedure of SAS. Results: COX2 immunostaining was scattered and restricted to cells in the stroma in bitches with NE. However, in bitches with endometritis, strong staining was observed in the luminal epithelium, glandular epithelium, and stromal cells. Staining was also observed in inflammatory cells localized in the stroma as well as inside of the glands. The percentage of COX2 positive stromal cells in bitches with AE, SE, and CE was significantly higher compared with NE (p < 0.005). In addition, the percentage of COX2 positive stromal cells in bitches with SE, and CE was significantly lower compared with AE (p < 0.003). Conclusion: COX2 could be involved in the pathophysiological mechanisms producing endometritis without the presence of cystic endometrial hyperplasia in bitches. However, further researches on this topic are required. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-14 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/136471 García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Alejandra; de la Sota, Rodolfo Luzbel; Stornelli, María Cecilia; Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 10; 2; 14-8-2020; 157-163 2226-4485 2218-6050 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/136471 |
identifier_str_mv |
García Mitacek, María Carla; Praderio, Romina Gisele; Stornelli, María Alejandra; de la Sota, Rodolfo Luzbel; Stornelli, María Cecilia; Endometritis in the bitch: Immunohistochemical localization of cyclooxygenase 2; University of Tripoli. Faculty of Veterinary Medicine; Open Veterinary Journal; 10; 2; 14-8-2020; 157-163 2226-4485 2218-6050 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.4314/ovj.v10i2.5 info:eu-repo/semantics/altIdentifier/url/https://www.ajol.info/index.php/ovj/article/view/198671 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
University of Tripoli. Faculty of Veterinary Medicine |
publisher.none.fl_str_mv |
University of Tripoli. Faculty of Veterinary Medicine |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614486246293504 |
score |
13.070432 |