The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas

Autores
Levi, L.; Castro-Parodi, M.; Martínez, N.; Piehl, L. L.; Rubín De Celis, E.; Herlax, Vanesa Silvana; Maté, Sabina María; Farina, M.; Damiano, A. E.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Syncytialization process is associated with a reduction in the number of caveolas, and a decreased of caveolin-1 (Cav-1). Differentiation of syncytiotrophoblast affects the membranes phospholipid composition. Thus, disturbances in these processes are related to pathological conditions such as preeclampsia. Objective To analyse the lipid composition of the apical (MVM) and the basal (BM) membranes of syncytiotrophoblast and its relationship with Cav-1 expression in normal and preeclamptic placentas. Molecular expression of Cav-1 was determined in MVM and BM from normal and preeclamptic placentas and in detergent-resistant membranes (DRMs). Phospholipids were analyzed by thin layer chromatography. Cholesterol was also determined by enzymatic assay. Membrane fluidity was evaluated by electron paramagnetic resonance. Sphingomyelin (SM) molecular species were analyzed and quantified by gas-liquid chromatography and mass spectrometry. Cav-1 was significantly reduced in MVM from preeclamptic placentas. Regarding Cav-1 localization, it was barely detectable in syncytiotrophoblast but it was present in the endothelium. Western blots also showed a significantly decrease of Cav-1 in the apical DRMs from preeclamptic placentas. Lipid analysis showed an increase SM in MVM from preeclamptic placentas without changes in cholesterol. Preeclamptic MVM fluidity decreased significantly and we found an increase in C18:1 fatty acids of SM. We concluded that preeclamptic-MVMs are more rigid than normal ones, possible due to an increment on SM. Moreover, the increase of long and unsaturated SM molecular specie found in these vesicles may disrupt the ability of SM to assemble into lipid rafts in the luminal leaflet of the bilayer, creating an unfavorable environment for Cav-1.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Ciencias Médicas
Caveolin-1
Human placenta
Membrane lipid composition
Preeclampsia
Trophoblast
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/86502
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/86502
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentasLevi, L.Castro-Parodi, M.Martínez, N.Piehl, L. L.Rubín De Celis, E.Herlax, Vanesa SilvanaMaté, Sabina MaríaFarina, M.Damiano, A. E.Ciencias MédicasCaveolin-1Human placentaMembrane lipid compositionPreeclampsiaTrophoblastSyncytialization process is associated with a reduction in the number of caveolas, and a decreased of caveolin-1 (Cav-1). Differentiation of syncytiotrophoblast affects the membranes phospholipid composition. Thus, disturbances in these processes are related to pathological conditions such as preeclampsia. Objective To analyse the lipid composition of the apical (MVM) and the basal (BM) membranes of syncytiotrophoblast and its relationship with Cav-1 expression in normal and preeclamptic placentas. Molecular expression of Cav-1 was determined in MVM and BM from normal and preeclamptic placentas and in detergent-resistant membranes (DRMs). Phospholipids were analyzed by thin layer chromatography. Cholesterol was also determined by enzymatic assay. Membrane fluidity was evaluated by electron paramagnetic resonance. Sphingomyelin (SM) molecular species were analyzed and quantified by gas-liquid chromatography and mass spectrometry. Cav-1 was significantly reduced in MVM from preeclamptic placentas. Regarding Cav-1 localization, it was barely detectable in syncytiotrophoblast but it was present in the endothelium. Western blots also showed a significantly decrease of Cav-1 in the apical DRMs from preeclamptic placentas. Lipid analysis showed an increase SM in MVM from preeclamptic placentas without changes in cholesterol. Preeclamptic MVM fluidity decreased significantly and we found an increase in C18:1 fatty acids of SM. We concluded that preeclamptic-MVMs are more rigid than normal ones, possible due to an increment on SM. Moreover, the increase of long and unsaturated SM molecular specie found in these vesicles may disrupt the ability of SM to assemble into lipid rafts in the luminal leaflet of the bilayer, creating an unfavorable environment for Cav-1.Instituto de Investigaciones Bioquímicas de La Plata2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf2171-2180http://sedici.unlp.edu.ar/handle/10915/86502enginfo:eu-repo/semantics/altIdentifier/issn/0005-2736info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2016.06.014info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:45Zoai:sedici.unlp.edu.ar:10915/86502Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:45.31SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
title The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
spellingShingle The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
Levi, L.
Ciencias Médicas
Caveolin-1
Human placenta
Membrane lipid composition
Preeclampsia
Trophoblast
title_short The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
title_full The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
title_fullStr The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
title_full_unstemmed The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
title_sort The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
dc.creator.none.fl_str_mv Levi, L.
Castro-Parodi, M.
Martínez, N.
Piehl, L. L.
Rubín De Celis, E.
Herlax, Vanesa Silvana
Maté, Sabina María
Farina, M.
Damiano, A. E.
author Levi, L.
author_facet Levi, L.
Castro-Parodi, M.
Martínez, N.
Piehl, L. L.
Rubín De Celis, E.
Herlax, Vanesa Silvana
Maté, Sabina María
Farina, M.
Damiano, A. E.
author_role author
author2 Castro-Parodi, M.
Martínez, N.
Piehl, L. L.
Rubín De Celis, E.
Herlax, Vanesa Silvana
Maté, Sabina María
Farina, M.
Damiano, A. E.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
Caveolin-1
Human placenta
Membrane lipid composition
Preeclampsia
Trophoblast
topic Ciencias Médicas
Caveolin-1
Human placenta
Membrane lipid composition
Preeclampsia
Trophoblast
dc.description.none.fl_txt_mv Syncytialization process is associated with a reduction in the number of caveolas, and a decreased of caveolin-1 (Cav-1). Differentiation of syncytiotrophoblast affects the membranes phospholipid composition. Thus, disturbances in these processes are related to pathological conditions such as preeclampsia. Objective To analyse the lipid composition of the apical (MVM) and the basal (BM) membranes of syncytiotrophoblast and its relationship with Cav-1 expression in normal and preeclamptic placentas. Molecular expression of Cav-1 was determined in MVM and BM from normal and preeclamptic placentas and in detergent-resistant membranes (DRMs). Phospholipids were analyzed by thin layer chromatography. Cholesterol was also determined by enzymatic assay. Membrane fluidity was evaluated by electron paramagnetic resonance. Sphingomyelin (SM) molecular species were analyzed and quantified by gas-liquid chromatography and mass spectrometry. Cav-1 was significantly reduced in MVM from preeclamptic placentas. Regarding Cav-1 localization, it was barely detectable in syncytiotrophoblast but it was present in the endothelium. Western blots also showed a significantly decrease of Cav-1 in the apical DRMs from preeclamptic placentas. Lipid analysis showed an increase SM in MVM from preeclamptic placentas without changes in cholesterol. Preeclamptic MVM fluidity decreased significantly and we found an increase in C18:1 fatty acids of SM. We concluded that preeclamptic-MVMs are more rigid than normal ones, possible due to an increment on SM. Moreover, the increase of long and unsaturated SM molecular specie found in these vesicles may disrupt the ability of SM to assemble into lipid rafts in the luminal leaflet of the bilayer, creating an unfavorable environment for Cav-1.
Instituto de Investigaciones Bioquímicas de La Plata
description Syncytialization process is associated with a reduction in the number of caveolas, and a decreased of caveolin-1 (Cav-1). Differentiation of syncytiotrophoblast affects the membranes phospholipid composition. Thus, disturbances in these processes are related to pathological conditions such as preeclampsia. Objective To analyse the lipid composition of the apical (MVM) and the basal (BM) membranes of syncytiotrophoblast and its relationship with Cav-1 expression in normal and preeclamptic placentas. Molecular expression of Cav-1 was determined in MVM and BM from normal and preeclamptic placentas and in detergent-resistant membranes (DRMs). Phospholipids were analyzed by thin layer chromatography. Cholesterol was also determined by enzymatic assay. Membrane fluidity was evaluated by electron paramagnetic resonance. Sphingomyelin (SM) molecular species were analyzed and quantified by gas-liquid chromatography and mass spectrometry. Cav-1 was significantly reduced in MVM from preeclamptic placentas. Regarding Cav-1 localization, it was barely detectable in syncytiotrophoblast but it was present in the endothelium. Western blots also showed a significantly decrease of Cav-1 in the apical DRMs from preeclamptic placentas. Lipid analysis showed an increase SM in MVM from preeclamptic placentas without changes in cholesterol. Preeclamptic MVM fluidity decreased significantly and we found an increase in C18:1 fatty acids of SM. We concluded that preeclamptic-MVMs are more rigid than normal ones, possible due to an increment on SM. Moreover, the increase of long and unsaturated SM molecular specie found in these vesicles may disrupt the ability of SM to assemble into lipid rafts in the luminal leaflet of the bilayer, creating an unfavorable environment for Cav-1.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/86502
url http://sedici.unlp.edu.ar/handle/10915/86502
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0005-2736
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2016.06.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
2171-2180
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institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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