The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas

Autores
Levi, Lorena Natalia; Castro-Parodi, Mauricio Omar; Martinez, Nora Alicia; Piehl, Lidia Leonor; Rubín de Celis, Emilio; Herlax, Vanesa Silvana; Maté, Sabina María; Farina, Mariana; Damiano, Alicia Ermelinda
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Syncytialization process is associated with a reduction in the number of caveolas, and a decreased of caveolin-1 (Cav-1). Differentiation of syncytiotrophoblast affects the membranes phospholipid composition. Thus, disturbances in these processes are related to pathological conditions such as preeclampsia.Objective: To analyse the lipid composition of the apical (MVM) and the basal (BM) membranes of syncytiotrophoblast and its relationshipwith Cav-1 expression in normal and preeclamptic placentas. Molecular expression of Cav-1 was determined inMVMand BMfrom normal and preeclamptic placentas and in detergent resistant membranes (DRMs). Phospholipids were analyzed by thin layer chromatography. Cholesterol was also determined by enzymatic assay. Membrane fluidity was evaluated by electron paramagnetic resonance.Sphingomyelin (SM) molecular species were analyzed and quantified by gas-liquid chromatography and mass spectrometry.Cav-1 was significantly reduced inMVMfrom preeclamptic placentas. Regarding Cav-1 localization, it was barely detectable in syncytiotrophoblast but it was present in the endothelium.Western blots also showed a significantly decrease of Cav-1 in the apical DRMs from preeclamptic placentas. Lipid analysis showed an increase SM in MVM from preeclamptic placentas without changes in cholesterol. Preeclamptic MVM fluidity decreased significantly and we found an increase in C18:1 fatty acids of SM. We concluded that preeclamptic-MVMs are more rigid than normal ones, possible due to an increment on SM. Moreover, the increase of long and unsaturated SM molecular specie found in these vesiclesmay disrupt the ability of SMto assemble into lipid rafts in the luminal leaflet of the bilayer, creating an unfavorable environment for Cav-1.
Fil: Levi, Lorena Natalia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Castro-Parodi, Mauricio Omar. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Martinez, Nora Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Piehl, Lidia Leonor. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Rubín de Celis, Emilio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Herlax, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Maté, Sabina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Farina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Damiano, Alicia Ermelinda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Trophoblast
Caveolin-1
Human Placenta
Preeclampsia
Membrane Lipid Composition
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47120
Acceder
id CONICETDig_7af4555f6bb8fd1bfe39109fae71fa75
oai_identifier_str oai:ri.conicet.gov.ar:11336/47120
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentasLevi, Lorena NataliaCastro-Parodi, Mauricio OmarMartinez, Nora AliciaPiehl, Lidia LeonorRubín de Celis, EmilioHerlax, Vanesa SilvanaMaté, Sabina MaríaFarina, MarianaDamiano, Alicia ErmelindaTrophoblastCaveolin-1Human PlacentaPreeclampsiaMembrane Lipid Compositionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Syncytialization process is associated with a reduction in the number of caveolas, and a decreased of caveolin-1 (Cav-1). Differentiation of syncytiotrophoblast affects the membranes phospholipid composition. Thus, disturbances in these processes are related to pathological conditions such as preeclampsia.Objective: To analyse the lipid composition of the apical (MVM) and the basal (BM) membranes of syncytiotrophoblast and its relationshipwith Cav-1 expression in normal and preeclamptic placentas. Molecular expression of Cav-1 was determined inMVMand BMfrom normal and preeclamptic placentas and in detergent resistant membranes (DRMs). Phospholipids were analyzed by thin layer chromatography. Cholesterol was also determined by enzymatic assay. Membrane fluidity was evaluated by electron paramagnetic resonance.Sphingomyelin (SM) molecular species were analyzed and quantified by gas-liquid chromatography and mass spectrometry.Cav-1 was significantly reduced inMVMfrom preeclamptic placentas. Regarding Cav-1 localization, it was barely detectable in syncytiotrophoblast but it was present in the endothelium.Western blots also showed a significantly decrease of Cav-1 in the apical DRMs from preeclamptic placentas. Lipid analysis showed an increase SM in MVM from preeclamptic placentas without changes in cholesterol. Preeclamptic MVM fluidity decreased significantly and we found an increase in C18:1 fatty acids of SM. We concluded that preeclamptic-MVMs are more rigid than normal ones, possible due to an increment on SM. Moreover, the increase of long and unsaturated SM molecular specie found in these vesiclesmay disrupt the ability of SMto assemble into lipid rafts in the luminal leaflet of the bilayer, creating an unfavorable environment for Cav-1.Fil: Levi, Lorena Natalia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Castro-Parodi, Mauricio Omar. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Martinez, Nora Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Piehl, Lidia Leonor. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Rubín de Celis, Emilio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Herlax, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Maté, Sabina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Farina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Damiano, Alicia Ermelinda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaElsevier Science2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47120Levi, Lorena Natalia; Castro-Parodi, Mauricio Omar; Martinez, Nora Alicia; Piehl, Lidia Leonor; Rubín de Celis, Emilio; et al.; The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1858; 9; 9-2016; 2171-21800005-2736CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2016.06.014info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S000527361630222Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:22Zoai:ri.conicet.gov.ar:11336/47120instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:23.096CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
title The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
spellingShingle The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
Levi, Lorena Natalia
Trophoblast
Caveolin-1
Human Placenta
Preeclampsia
Membrane Lipid Composition
title_short The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
title_full The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
title_fullStr The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
title_full_unstemmed The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
title_sort The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas
dc.creator.none.fl_str_mv Levi, Lorena Natalia
Castro-Parodi, Mauricio Omar
Martinez, Nora Alicia
Piehl, Lidia Leonor
Rubín de Celis, Emilio
Herlax, Vanesa Silvana
Maté, Sabina María
Farina, Mariana
Damiano, Alicia Ermelinda
author Levi, Lorena Natalia
author_facet Levi, Lorena Natalia
Castro-Parodi, Mauricio Omar
Martinez, Nora Alicia
Piehl, Lidia Leonor
Rubín de Celis, Emilio
Herlax, Vanesa Silvana
Maté, Sabina María
Farina, Mariana
Damiano, Alicia Ermelinda
author_role author
author2 Castro-Parodi, Mauricio Omar
Martinez, Nora Alicia
Piehl, Lidia Leonor
Rubín de Celis, Emilio
Herlax, Vanesa Silvana
Maté, Sabina María
Farina, Mariana
Damiano, Alicia Ermelinda
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trophoblast
Caveolin-1
Human Placenta
Preeclampsia
Membrane Lipid Composition
topic Trophoblast
Caveolin-1
Human Placenta
Preeclampsia
Membrane Lipid Composition
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Syncytialization process is associated with a reduction in the number of caveolas, and a decreased of caveolin-1 (Cav-1). Differentiation of syncytiotrophoblast affects the membranes phospholipid composition. Thus, disturbances in these processes are related to pathological conditions such as preeclampsia.Objective: To analyse the lipid composition of the apical (MVM) and the basal (BM) membranes of syncytiotrophoblast and its relationshipwith Cav-1 expression in normal and preeclamptic placentas. Molecular expression of Cav-1 was determined inMVMand BMfrom normal and preeclamptic placentas and in detergent resistant membranes (DRMs). Phospholipids were analyzed by thin layer chromatography. Cholesterol was also determined by enzymatic assay. Membrane fluidity was evaluated by electron paramagnetic resonance.Sphingomyelin (SM) molecular species were analyzed and quantified by gas-liquid chromatography and mass spectrometry.Cav-1 was significantly reduced inMVMfrom preeclamptic placentas. Regarding Cav-1 localization, it was barely detectable in syncytiotrophoblast but it was present in the endothelium.Western blots also showed a significantly decrease of Cav-1 in the apical DRMs from preeclamptic placentas. Lipid analysis showed an increase SM in MVM from preeclamptic placentas without changes in cholesterol. Preeclamptic MVM fluidity decreased significantly and we found an increase in C18:1 fatty acids of SM. We concluded that preeclamptic-MVMs are more rigid than normal ones, possible due to an increment on SM. Moreover, the increase of long and unsaturated SM molecular specie found in these vesiclesmay disrupt the ability of SMto assemble into lipid rafts in the luminal leaflet of the bilayer, creating an unfavorable environment for Cav-1.
Fil: Levi, Lorena Natalia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Castro-Parodi, Mauricio Omar. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Martinez, Nora Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Piehl, Lidia Leonor. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Rubín de Celis, Emilio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Herlax, Vanesa Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Maté, Sabina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
Fil: Farina, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Damiano, Alicia Ermelinda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Syncytialization process is associated with a reduction in the number of caveolas, and a decreased of caveolin-1 (Cav-1). Differentiation of syncytiotrophoblast affects the membranes phospholipid composition. Thus, disturbances in these processes are related to pathological conditions such as preeclampsia.Objective: To analyse the lipid composition of the apical (MVM) and the basal (BM) membranes of syncytiotrophoblast and its relationshipwith Cav-1 expression in normal and preeclamptic placentas. Molecular expression of Cav-1 was determined inMVMand BMfrom normal and preeclamptic placentas and in detergent resistant membranes (DRMs). Phospholipids were analyzed by thin layer chromatography. Cholesterol was also determined by enzymatic assay. Membrane fluidity was evaluated by electron paramagnetic resonance.Sphingomyelin (SM) molecular species were analyzed and quantified by gas-liquid chromatography and mass spectrometry.Cav-1 was significantly reduced inMVMfrom preeclamptic placentas. Regarding Cav-1 localization, it was barely detectable in syncytiotrophoblast but it was present in the endothelium.Western blots also showed a significantly decrease of Cav-1 in the apical DRMs from preeclamptic placentas. Lipid analysis showed an increase SM in MVM from preeclamptic placentas without changes in cholesterol. Preeclamptic MVM fluidity decreased significantly and we found an increase in C18:1 fatty acids of SM. We concluded that preeclamptic-MVMs are more rigid than normal ones, possible due to an increment on SM. Moreover, the increase of long and unsaturated SM molecular specie found in these vesiclesmay disrupt the ability of SMto assemble into lipid rafts in the luminal leaflet of the bilayer, creating an unfavorable environment for Cav-1.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47120
Levi, Lorena Natalia; Castro-Parodi, Mauricio Omar; Martinez, Nora Alicia; Piehl, Lidia Leonor; Rubín de Celis, Emilio; et al.; The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1858; 9; 9-2016; 2171-2180
0005-2736
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47120
identifier_str_mv Levi, Lorena Natalia; Castro-Parodi, Mauricio Omar; Martinez, Nora Alicia; Piehl, Lidia Leonor; Rubín de Celis, Emilio; et al.; The unfavorable lipid environment reduced caveolin-1 expression in apical membranes from human preeclamptic placentas; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1858; 9; 9-2016; 2171-2180
0005-2736
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbamem.2016.06.014
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S000527361630222X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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