Long-term preservation of Lotus tenuis adventitious buds

Autores
Espasandin, Fabiana Daniela; Brugnoli, Elsa Andrea; Ayala, Paula Gabriela; Ayala, Lilian P.; Ruiz, Oscar Adolfo; Sansberro, Pedro Alfonso
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Espasandin, Fabiana Daniela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Brugnoli, Elsa Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Ayala, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Ayala, Paula Gabriela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Ayala, Lilián P. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Ayala, Lilián P. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Biotecnología; Argentina.
Fil: Ruiz, Oscar Adolfo. Instituto de Fisiología y Recursos Genéticos Vegetales “Ing. Victorio S. Trippi”; Argentina.
Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Encapsulation-dehydration, encapsulation-vitrification, and vitrification were tested for cryopreservation of Lotus tenuis (Fagaceae) adventitious buds clusters (ABCs) obtained by a direct regeneration system from leaves cultures. Among them, the PVS3-based vitrification procedure was found to be useful for survival and regrowth of the preserved explants. For vitrification, the ABCs were dehydrated in a solution containing 2 M glycerol + 0.4 M sucrose for 25 min at room temperature, submerged in PVS3 solution for 1 h at 0 °C, then immersed in liquid nitrogen for 48 h and rapidly rewarmed. Afterword, the explants were unloaded in MS liquid medium with 1.2 M sucrose for 30 min. The washed tissues were dried superficially on filter paper and cultured in semisolid hormone-free MS medium containing 0.1 M sucrose. All cultures were maintained at 25 °C in the dark for 10 days and transferred to the light conditions. With this procedure, 79 ± 5.3% survival and more than 80% of the plantlets displaying a phenotype similar to the non-treated control after acclimatization. The data settled from ISSR showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues and the non-treated plants. Thus, our results indicate that the use of vitrification-based PVS3 solution offers a simple, accurate, and appropriate procedure for the cryopreservation of L. tenuis adventitious buds.
Fuente
Plant Cell, Tissue and Organ Culture, 2019, no. 136, p. 373-382.
Materia
Cryopreservation
PVS3
Vitrification
ISSR markers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/27779
Acceder
id RIUNNE_ea2cced149f0a71cd83c8a1f3b8273d1
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/27779
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Long-term preservation of Lotus tenuis adventitious budsEspasandin, Fabiana DanielaBrugnoli, Elsa AndreaAyala, Paula GabrielaAyala, Lilian P.Ruiz, Oscar AdolfoSansberro, Pedro AlfonsoCryopreservationPVS3VitrificationISSR markersFil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Espasandin, Fabiana Daniela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Brugnoli, Elsa Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Ayala, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Ayala, Paula Gabriela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Ayala, Lilián P. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Ayala, Lilián P. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Biotecnología; Argentina.Fil: Ruiz, Oscar Adolfo. Instituto de Fisiología y Recursos Genéticos Vegetales “Ing. Victorio S. Trippi”; Argentina.Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Encapsulation-dehydration, encapsulation-vitrification, and vitrification were tested for cryopreservation of Lotus tenuis (Fagaceae) adventitious buds clusters (ABCs) obtained by a direct regeneration system from leaves cultures. Among them, the PVS3-based vitrification procedure was found to be useful for survival and regrowth of the preserved explants. For vitrification, the ABCs were dehydrated in a solution containing 2 M glycerol + 0.4 M sucrose for 25 min at room temperature, submerged in PVS3 solution for 1 h at 0 °C, then immersed in liquid nitrogen for 48 h and rapidly rewarmed. Afterword, the explants were unloaded in MS liquid medium with 1.2 M sucrose for 30 min. The washed tissues were dried superficially on filter paper and cultured in semisolid hormone-free MS medium containing 0.1 M sucrose. All cultures were maintained at 25 °C in the dark for 10 days and transferred to the light conditions. With this procedure, 79 ± 5.3% survival and more than 80% of the plantlets displaying a phenotype similar to the non-treated control after acclimatization. The data settled from ISSR showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues and the non-treated plants. Thus, our results indicate that the use of vitrification-based PVS3 solution offers a simple, accurate, and appropriate procedure for the cryopreservation of L. tenuis adventitious buds.Springer Nature2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfEspasandin, Fabiana Daniela, et al., 2019. Long-term preservation of Lotus tenuis adventitious buds. Plant Cell, Tissue and Organ Culture. Suiza: Springer Nature, no. 136, p. 373-382. ISSN 1573-5044.0167-6857http://repositorio.unne.edu.ar/handle/123456789/27779Plant Cell, Tissue and Organ Culture, 2019, no. 136, p. 373-382.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://www.springer.com/journal/11240info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-11T10:51:22Zoai:repositorio.unne.edu.ar:123456789/27779instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-11 10:51:23.134Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Long-term preservation of Lotus tenuis adventitious buds
title Long-term preservation of Lotus tenuis adventitious buds
spellingShingle Long-term preservation of Lotus tenuis adventitious buds
Espasandin, Fabiana Daniela
Cryopreservation
PVS3
Vitrification
ISSR markers
title_short Long-term preservation of Lotus tenuis adventitious buds
title_full Long-term preservation of Lotus tenuis adventitious buds
title_fullStr Long-term preservation of Lotus tenuis adventitious buds
title_full_unstemmed Long-term preservation of Lotus tenuis adventitious buds
title_sort Long-term preservation of Lotus tenuis adventitious buds
dc.creator.none.fl_str_mv Espasandin, Fabiana Daniela
Brugnoli, Elsa Andrea
Ayala, Paula Gabriela
Ayala, Lilian P.
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author Espasandin, Fabiana Daniela
author_facet Espasandin, Fabiana Daniela
Brugnoli, Elsa Andrea
Ayala, Paula Gabriela
Ayala, Lilian P.
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author_role author
author2 Brugnoli, Elsa Andrea
Ayala, Paula Gabriela
Ayala, Lilian P.
Ruiz, Oscar Adolfo
Sansberro, Pedro Alfonso
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cryopreservation
PVS3
Vitrification
ISSR markers
topic Cryopreservation
PVS3
Vitrification
ISSR markers
dc.description.none.fl_txt_mv Fil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Espasandin, Fabiana Daniela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Brugnoli, Elsa Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Brugnoli, Elsa Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Ayala, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Ayala, Paula Gabriela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Ayala, Lilián P. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Ayala, Lilián P. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Biotecnología; Argentina.
Fil: Ruiz, Oscar Adolfo. Instituto de Fisiología y Recursos Genéticos Vegetales “Ing. Victorio S. Trippi”; Argentina.
Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Encapsulation-dehydration, encapsulation-vitrification, and vitrification were tested for cryopreservation of Lotus tenuis (Fagaceae) adventitious buds clusters (ABCs) obtained by a direct regeneration system from leaves cultures. Among them, the PVS3-based vitrification procedure was found to be useful for survival and regrowth of the preserved explants. For vitrification, the ABCs were dehydrated in a solution containing 2 M glycerol + 0.4 M sucrose for 25 min at room temperature, submerged in PVS3 solution for 1 h at 0 °C, then immersed in liquid nitrogen for 48 h and rapidly rewarmed. Afterword, the explants were unloaded in MS liquid medium with 1.2 M sucrose for 30 min. The washed tissues were dried superficially on filter paper and cultured in semisolid hormone-free MS medium containing 0.1 M sucrose. All cultures were maintained at 25 °C in the dark for 10 days and transferred to the light conditions. With this procedure, 79 ± 5.3% survival and more than 80% of the plantlets displaying a phenotype similar to the non-treated control after acclimatization. The data settled from ISSR showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues and the non-treated plants. Thus, our results indicate that the use of vitrification-based PVS3 solution offers a simple, accurate, and appropriate procedure for the cryopreservation of L. tenuis adventitious buds.
description Fil: Espasandin, Fabiana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Espasandin, Fabiana Daniela, et al., 2019. Long-term preservation of Lotus tenuis adventitious buds. Plant Cell, Tissue and Organ Culture. Suiza: Springer Nature, no. 136, p. 373-382. ISSN 1573-5044.
0167-6857
http://repositorio.unne.edu.ar/handle/123456789/27779
identifier_str_mv Espasandin, Fabiana Daniela, et al., 2019. Long-term preservation of Lotus tenuis adventitious buds. Plant Cell, Tissue and Organ Culture. Suiza: Springer Nature, no. 136, p. 373-382. ISSN 1573-5044.
0167-6857
url http://repositorio.unne.edu.ar/handle/123456789/27779
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.springer.com/journal/11240
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv Plant Cell, Tissue and Organ Culture, 2019, no. 136, p. 373-382.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 12.993085

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