Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification

Autores
Fuller, Barry J.; Petrenko, Alexander Y.; Rodriguez, Joaquin Valentin; Somov, Alexander Y.; Balaban, Cecilia Lucía; Guibert, Edgardo Elvio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Isolated liver cells (primarily isolated hepatocytes) have found important applications in science and medicine over the past 40 years in a wide range of areas, including physiological studies, investigations on liver metabolism, organ preservation and drug de-toxification, experimental and clinical transplantation. An integral component of many of these works is the need to store the isolated cells, either for short or long-term periods. This review covers the biopreservation of liver cells, with a focus on the history of liver cell biopreservation, the application of hypothermia for short-term storage, standard cryopreservation methods for isolated hepatocytes, the biopreservation of other types of liver cells, and recent developments such as vitrification of hepatocytes. By understanding the basis for the different approaches, it will be possible to select the best options for liver cell biopreservation in different applications, and identify ways to improve preservation protocols for the future.
Fil: Fuller, Barry J.. University College London; Estados Unidos
Fil: Petrenko, Alexander Y.. Ukraine Academy of Sciences; Ucrania
Fil: Rodriguez, Joaquin Valentin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina
Fil: Somov, Alexander Y.. Ukraine Academy of Sciences; Ucrania
Fil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Guibert, Edgardo Elvio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina
Materia
ISOLATED HEPATOCYTES
ISOLATED LIVER CELLS
BIOPRESERVATION
HYPOTHERMIC STORAGE
CRYOPRESERVATION
VITRIFICATION
SUBZERO NON FREEZING STORAGE
DRYING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101132
Acceder
id CONICETDig_2a240831e98f7ef6a890a487f29ab673
oai_identifier_str oai:ri.conicet.gov.ar:11336/101132
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrificationFuller, Barry J.Petrenko, Alexander Y.Rodriguez, Joaquin ValentinSomov, Alexander Y.Balaban, Cecilia LucíaGuibert, Edgardo ElvioISOLATED HEPATOCYTESISOLATED LIVER CELLSBIOPRESERVATIONHYPOTHERMIC STORAGECRYOPRESERVATIONVITRIFICATIONSUBZERO NON FREEZING STORAGEDRYINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Isolated liver cells (primarily isolated hepatocytes) have found important applications in science and medicine over the past 40 years in a wide range of areas, including physiological studies, investigations on liver metabolism, organ preservation and drug de-toxification, experimental and clinical transplantation. An integral component of many of these works is the need to store the isolated cells, either for short or long-term periods. This review covers the biopreservation of liver cells, with a focus on the history of liver cell biopreservation, the application of hypothermia for short-term storage, standard cryopreservation methods for isolated hepatocytes, the biopreservation of other types of liver cells, and recent developments such as vitrification of hepatocytes. By understanding the basis for the different approaches, it will be possible to select the best options for liver cell biopreservation in different applications, and identify ways to improve preservation protocols for the future.Fil: Fuller, Barry J.. University College London; Estados UnidosFil: Petrenko, Alexander Y.. Ukraine Academy of Sciences; UcraniaFil: Rodriguez, Joaquin Valentin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; ArgentinaFil: Somov, Alexander Y.. Ukraine Academy of Sciences; UcraniaFil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Guibert, Edgardo Elvio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; ArgentinaCryo Letters2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101132Fuller, Barry J.; Petrenko, Alexander Y.; Rodriguez, Joaquin Valentin; Somov, Alexander Y.; Balaban, Cecilia Lucía; et al.; Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification; Cryo Letters; Cryo-letters; 34; 4; 7-2013; 432-4520143-20441742-0644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.cryoletters.org/Abstracts/vol_34_4_2013.htminfo:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/contentone/cryo/cryo/2013/00000034/00000004/art00013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:19Zoai:ri.conicet.gov.ar:11336/101132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:19.637CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification
title Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification
spellingShingle Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification
Fuller, Barry J.
ISOLATED HEPATOCYTES
ISOLATED LIVER CELLS
BIOPRESERVATION
HYPOTHERMIC STORAGE
CRYOPRESERVATION
VITRIFICATION
SUBZERO NON FREEZING STORAGE
DRYING
title_short Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification
title_full Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification
title_fullStr Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification
title_full_unstemmed Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification
title_sort Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification
dc.creator.none.fl_str_mv Fuller, Barry J.
Petrenko, Alexander Y.
Rodriguez, Joaquin Valentin
Somov, Alexander Y.
Balaban, Cecilia Lucía
Guibert, Edgardo Elvio
author Fuller, Barry J.
author_facet Fuller, Barry J.
Petrenko, Alexander Y.
Rodriguez, Joaquin Valentin
Somov, Alexander Y.
Balaban, Cecilia Lucía
Guibert, Edgardo Elvio
author_role author
author2 Petrenko, Alexander Y.
Rodriguez, Joaquin Valentin
Somov, Alexander Y.
Balaban, Cecilia Lucía
Guibert, Edgardo Elvio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ISOLATED HEPATOCYTES
ISOLATED LIVER CELLS
BIOPRESERVATION
HYPOTHERMIC STORAGE
CRYOPRESERVATION
VITRIFICATION
SUBZERO NON FREEZING STORAGE
DRYING
topic ISOLATED HEPATOCYTES
ISOLATED LIVER CELLS
BIOPRESERVATION
HYPOTHERMIC STORAGE
CRYOPRESERVATION
VITRIFICATION
SUBZERO NON FREEZING STORAGE
DRYING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Isolated liver cells (primarily isolated hepatocytes) have found important applications in science and medicine over the past 40 years in a wide range of areas, including physiological studies, investigations on liver metabolism, organ preservation and drug de-toxification, experimental and clinical transplantation. An integral component of many of these works is the need to store the isolated cells, either for short or long-term periods. This review covers the biopreservation of liver cells, with a focus on the history of liver cell biopreservation, the application of hypothermia for short-term storage, standard cryopreservation methods for isolated hepatocytes, the biopreservation of other types of liver cells, and recent developments such as vitrification of hepatocytes. By understanding the basis for the different approaches, it will be possible to select the best options for liver cell biopreservation in different applications, and identify ways to improve preservation protocols for the future.
Fil: Fuller, Barry J.. University College London; Estados Unidos
Fil: Petrenko, Alexander Y.. Ukraine Academy of Sciences; Ucrania
Fil: Rodriguez, Joaquin Valentin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina
Fil: Somov, Alexander Y.. Ukraine Academy of Sciences; Ucrania
Fil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Guibert, Edgardo Elvio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Secretaria de Ciencia y Técnica. Centro Binacional de Investigación en Criobiología Clínica y Aplicada; Argentina
description Isolated liver cells (primarily isolated hepatocytes) have found important applications in science and medicine over the past 40 years in a wide range of areas, including physiological studies, investigations on liver metabolism, organ preservation and drug de-toxification, experimental and clinical transplantation. An integral component of many of these works is the need to store the isolated cells, either for short or long-term periods. This review covers the biopreservation of liver cells, with a focus on the history of liver cell biopreservation, the application of hypothermia for short-term storage, standard cryopreservation methods for isolated hepatocytes, the biopreservation of other types of liver cells, and recent developments such as vitrification of hepatocytes. By understanding the basis for the different approaches, it will be possible to select the best options for liver cell biopreservation in different applications, and identify ways to improve preservation protocols for the future.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101132
Fuller, Barry J.; Petrenko, Alexander Y.; Rodriguez, Joaquin Valentin; Somov, Alexander Y.; Balaban, Cecilia Lucía; et al.; Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification; Cryo Letters; Cryo-letters; 34; 4; 7-2013; 432-452
0143-2044
1742-0644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101132
identifier_str_mv Fuller, Barry J.; Petrenko, Alexander Y.; Rodriguez, Joaquin Valentin; Somov, Alexander Y.; Balaban, Cecilia Lucía; et al.; Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification; Cryo Letters; Cryo-letters; 34; 4; 7-2013; 432-452
0143-2044
1742-0644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.cryoletters.org/Abstracts/vol_34_4_2013.htm
info:eu-repo/semantics/altIdentifier/url/https://www.ingentaconnect.com/contentone/cryo/cryo/2013/00000034/00000004/art00013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cryo Letters
publisher.none.fl_str_mv Cryo Letters
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269278616682496
score 13.13397

Publicaciones similares