Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures

Autores
Lakey, Jonathan; Guo, Wenyi; Bluguermann, Carolina; Tucker, Tori; Mutto, Adrián Angel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In recent years, stem cell therapy has been applied to the treatment of many diseases. Stem cells are extensively researched fortheir potential to replace diseased tissues in conditions such as degenerative diseases, tumors, trauma, and others, collectively knownas regenerative therapy. However, as age advances, the number and quality of stem cells decreases; by the age of 50, a person has lessthan 3% of the stem cells they had at birth. Although stem cells can be preserved in vitro for long periods of time, their activity aftercryopreservation and recovery does not reach the level expected under current technological conditions. Therefore, it is particularlyimportant to find a more suitable freezing method. In this paper, we present a novel method for freezing stem cells that ensures highcellular activity after recovery. In this study we validate a combinational approach of using two permeating cryoprotective agents, atlower than normal concentrations resulting in higher recovery post cryopreservation of human mesenchymal stem cells.
Fil: Lakey, Jonathan. University of California at Irvine; Estados Unidos
Fil: Guo, Wenyi. Qilu Hospital of Shandong University; China
Fil: Bluguermann, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Tucker, Tori. University of California at Irvine; Estados Unidos
Fil: Mutto, Adrián Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Materia
MSC
CRyopreservation
Vitrification
Diagnosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/265508
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Novel Methods to Preserve Mesenchymal Stem Cells at low TemperaturesLakey, JonathanGuo, WenyiBluguermann, CarolinaTucker, ToriMutto, Adrián AngelMSCCRyopreservationVitrificationDiagnosishttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4In recent years, stem cell therapy has been applied to the treatment of many diseases. Stem cells are extensively researched fortheir potential to replace diseased tissues in conditions such as degenerative diseases, tumors, trauma, and others, collectively knownas regenerative therapy. However, as age advances, the number and quality of stem cells decreases; by the age of 50, a person has lessthan 3% of the stem cells they had at birth. Although stem cells can be preserved in vitro for long periods of time, their activity aftercryopreservation and recovery does not reach the level expected under current technological conditions. Therefore, it is particularlyimportant to find a more suitable freezing method. In this paper, we present a novel method for freezing stem cells that ensures highcellular activity after recovery. In this study we validate a combinational approach of using two permeating cryoprotective agents, atlower than normal concentrations resulting in higher recovery post cryopreservation of human mesenchymal stem cells.Fil: Lakey, Jonathan. University of California at Irvine; Estados UnidosFil: Guo, Wenyi. Qilu Hospital of Shandong University; ChinaFil: Bluguermann, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Tucker, Tori. University of California at Irvine; Estados UnidosFil: Mutto, Adrián Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaBiomedGrid2024-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/265508Lakey, Jonathan; Guo, Wenyi; Bluguermann, Carolina; Tucker, Tori; Mutto, Adrián Angel; Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures; BiomedGrid; American Journal of Biomedical Science & Research; 22; 1; 4-2024; 137-1412642-1747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.34297/AJBSR.2024.22.002926info:eu-repo/semantics/altIdentifier/url/https://biomedgrid.com/pdf/AJBSR.MS.ID.002926.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:08:51Zoai:ri.conicet.gov.ar:11336/265508instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:08:51.879CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures
title Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures
spellingShingle Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures
Lakey, Jonathan
MSC
CRyopreservation
Vitrification
Diagnosis
title_short Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures
title_full Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures
title_fullStr Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures
title_full_unstemmed Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures
title_sort Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures
dc.creator.none.fl_str_mv Lakey, Jonathan
Guo, Wenyi
Bluguermann, Carolina
Tucker, Tori
Mutto, Adrián Angel
author Lakey, Jonathan
author_facet Lakey, Jonathan
Guo, Wenyi
Bluguermann, Carolina
Tucker, Tori
Mutto, Adrián Angel
author_role author
author2 Guo, Wenyi
Bluguermann, Carolina
Tucker, Tori
Mutto, Adrián Angel
author2_role author
author
author
author
dc.subject.none.fl_str_mv MSC
CRyopreservation
Vitrification
Diagnosis
topic MSC
CRyopreservation
Vitrification
Diagnosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In recent years, stem cell therapy has been applied to the treatment of many diseases. Stem cells are extensively researched fortheir potential to replace diseased tissues in conditions such as degenerative diseases, tumors, trauma, and others, collectively knownas regenerative therapy. However, as age advances, the number and quality of stem cells decreases; by the age of 50, a person has lessthan 3% of the stem cells they had at birth. Although stem cells can be preserved in vitro for long periods of time, their activity aftercryopreservation and recovery does not reach the level expected under current technological conditions. Therefore, it is particularlyimportant to find a more suitable freezing method. In this paper, we present a novel method for freezing stem cells that ensures highcellular activity after recovery. In this study we validate a combinational approach of using two permeating cryoprotective agents, atlower than normal concentrations resulting in higher recovery post cryopreservation of human mesenchymal stem cells.
Fil: Lakey, Jonathan. University of California at Irvine; Estados Unidos
Fil: Guo, Wenyi. Qilu Hospital of Shandong University; China
Fil: Bluguermann, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Tucker, Tori. University of California at Irvine; Estados Unidos
Fil: Mutto, Adrián Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
description In recent years, stem cell therapy has been applied to the treatment of many diseases. Stem cells are extensively researched fortheir potential to replace diseased tissues in conditions such as degenerative diseases, tumors, trauma, and others, collectively knownas regenerative therapy. However, as age advances, the number and quality of stem cells decreases; by the age of 50, a person has lessthan 3% of the stem cells they had at birth. Although stem cells can be preserved in vitro for long periods of time, their activity aftercryopreservation and recovery does not reach the level expected under current technological conditions. Therefore, it is particularlyimportant to find a more suitable freezing method. In this paper, we present a novel method for freezing stem cells that ensures highcellular activity after recovery. In this study we validate a combinational approach of using two permeating cryoprotective agents, atlower than normal concentrations resulting in higher recovery post cryopreservation of human mesenchymal stem cells.
publishDate 2024
dc.date.none.fl_str_mv 2024-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/265508
Lakey, Jonathan; Guo, Wenyi; Bluguermann, Carolina; Tucker, Tori; Mutto, Adrián Angel; Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures; BiomedGrid; American Journal of Biomedical Science & Research; 22; 1; 4-2024; 137-141
2642-1747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/265508
identifier_str_mv Lakey, Jonathan; Guo, Wenyi; Bluguermann, Carolina; Tucker, Tori; Mutto, Adrián Angel; Novel Methods to Preserve Mesenchymal Stem Cells at low Temperatures; BiomedGrid; American Journal of Biomedical Science & Research; 22; 1; 4-2024; 137-141
2642-1747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.34297/AJBSR.2024.22.002926
info:eu-repo/semantics/altIdentifier/url/https://biomedgrid.com/pdf/AJBSR.MS.ID.002926.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BiomedGrid
publisher.none.fl_str_mv BiomedGrid
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980426723885056
score 12.993085

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