Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)

Autores
Cholich, Luciana Andrea; Pistán, María Elena; Torres, Ana María; Ortega, Hugo Héctor; Gardner, Dale R.; Bustillo, Soledad
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Pistán, María Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina.
Fil: Gardner, Dale R. Agricultural Research Service U.S. Departament of Agriculture; Estados Unidos.
Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Introduction: Prosopis spp. pods have shown to be a potential source of protein and energy in livestock. However, prolonged ingestion of some of these species produces neurological symptoms in ruminants. Objective: In the present study, the alkaloid content and the in vitro neurotoxic activity of alkaloid enrichedextracts from P. flexuosa and P. nigra pods were determined in order to elucidate the mechanism of animal poisoning caused by these species. Methods: The main alkaloids present in both extracts were analysed by high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS). The cytotoxic activity of Prosopis alkaloid enriched-extracts in primary mixed glial cell culture was assessed by phase contrast microscopy and using neutral red, and lactate dehydrogenase (LDH) activity assays. Results: Juliprosine and juliprosopine were identified in P. flexuosa pods, while the absence of these alkaloids in P. nigra was confirmed. Both extracts (5-30 μg/mL) induced in a dose dependent manner, morphological alterations, such as swelling, enlargement and detachment from the culture surface. Consistent with this, decrease in cell viability and release of LDH 48 hours after exposure, revealed that P. flexuosa pods was significantly more cytotoxic than P. nigra. Conclusions: In P. flexuosa pods, juliprosine and juliprosopine alkaloids were identified for the first time. Moreover, the present study suggests that the cytotoxic effect displayed by both extracts is due to its alkaloid content. However, the presence of piperidine alkaloids in P. flexuosa could explain the greater cytotoxicity on glial cells with respect to P. nigra that was not shown to contain these alkaloids
Fuente
Revista de Biología Tropical, 2021, vol. 69, no. 1, p. 197-206.
Materia
Algarrobos
Cytotoxicity
Piperidine alkaloids
Prosopis species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/53153

id RIUNNE_e7a5a37088995ad71e19ce4c7b4ce6af
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/53153
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)Cholich, Luciana AndreaPistán, María ElenaTorres, Ana MaríaOrtega, Hugo HéctorGardner, Dale R.Bustillo, SoledadAlgarrobosCytotoxicityPiperidine alkaloidsProsopis speciesFil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Pistán, María Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina.Fil: Gardner, Dale R. Agricultural Research Service U.S. Departament of Agriculture; Estados Unidos.Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.Introduction: Prosopis spp. pods have shown to be a potential source of protein and energy in livestock. However, prolonged ingestion of some of these species produces neurological symptoms in ruminants. Objective: In the present study, the alkaloid content and the in vitro neurotoxic activity of alkaloid enrichedextracts from P. flexuosa and P. nigra pods were determined in order to elucidate the mechanism of animal poisoning caused by these species. Methods: The main alkaloids present in both extracts were analysed by high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS). The cytotoxic activity of Prosopis alkaloid enriched-extracts in primary mixed glial cell culture was assessed by phase contrast microscopy and using neutral red, and lactate dehydrogenase (LDH) activity assays. Results: Juliprosine and juliprosopine were identified in P. flexuosa pods, while the absence of these alkaloids in P. nigra was confirmed. Both extracts (5-30 μg/mL) induced in a dose dependent manner, morphological alterations, such as swelling, enlargement and detachment from the culture surface. Consistent with this, decrease in cell viability and release of LDH 48 hours after exposure, revealed that P. flexuosa pods was significantly more cytotoxic than P. nigra. Conclusions: In P. flexuosa pods, juliprosine and juliprosopine alkaloids were identified for the first time. Moreover, the present study suggests that the cytotoxic effect displayed by both extracts is due to its alkaloid content. However, the presence of piperidine alkaloids in P. flexuosa could explain the greater cytotoxicity on glial cells with respect to P. nigra that was not shown to contain these alkaloidsUniversidad de Costa Rica2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 197-206application/pdfCholich, Luciana Andrea., et. al., 2021. Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae). Revista de Biología Tropical. San José: Universidad de Costa Rica, vol. 69, no. 1, p. 197-2060. E-ISSN 2215-2075.0034-7744http://repositorio.unne.edu.ar/handle/123456789/53153Revista de Biología Tropical, 2021, vol. 69, no. 1, p. 197-206.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:29:37Zoai:repositorio.unne.edu.ar:123456789/53153instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:29:37.537Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
title Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
spellingShingle Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
Cholich, Luciana Andrea
Algarrobos
Cytotoxicity
Piperidine alkaloids
Prosopis species
title_short Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
title_full Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
title_fullStr Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
title_full_unstemmed Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
title_sort Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae)
dc.creator.none.fl_str_mv Cholich, Luciana Andrea
Pistán, María Elena
Torres, Ana María
Ortega, Hugo Héctor
Gardner, Dale R.
Bustillo, Soledad
author Cholich, Luciana Andrea
author_facet Cholich, Luciana Andrea
Pistán, María Elena
Torres, Ana María
Ortega, Hugo Héctor
Gardner, Dale R.
Bustillo, Soledad
author_role author
author2 Pistán, María Elena
Torres, Ana María
Ortega, Hugo Héctor
Gardner, Dale R.
Bustillo, Soledad
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Algarrobos
Cytotoxicity
Piperidine alkaloids
Prosopis species
topic Algarrobos
Cytotoxicity
Piperidine alkaloids
Prosopis species
dc.description.none.fl_txt_mv Fil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Pistán, María Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina.
Fil: Gardner, Dale R. Agricultural Research Service U.S. Departament of Agriculture; Estados Unidos.
Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Introduction: Prosopis spp. pods have shown to be a potential source of protein and energy in livestock. However, prolonged ingestion of some of these species produces neurological symptoms in ruminants. Objective: In the present study, the alkaloid content and the in vitro neurotoxic activity of alkaloid enrichedextracts from P. flexuosa and P. nigra pods were determined in order to elucidate the mechanism of animal poisoning caused by these species. Methods: The main alkaloids present in both extracts were analysed by high performance liquid chromatography-high resolution mass spectrometry (HPLC-HRMS). The cytotoxic activity of Prosopis alkaloid enriched-extracts in primary mixed glial cell culture was assessed by phase contrast microscopy and using neutral red, and lactate dehydrogenase (LDH) activity assays. Results: Juliprosine and juliprosopine were identified in P. flexuosa pods, while the absence of these alkaloids in P. nigra was confirmed. Both extracts (5-30 μg/mL) induced in a dose dependent manner, morphological alterations, such as swelling, enlargement and detachment from the culture surface. Consistent with this, decrease in cell viability and release of LDH 48 hours after exposure, revealed that P. flexuosa pods was significantly more cytotoxic than P. nigra. Conclusions: In P. flexuosa pods, juliprosine and juliprosopine alkaloids were identified for the first time. Moreover, the present study suggests that the cytotoxic effect displayed by both extracts is due to its alkaloid content. However, the presence of piperidine alkaloids in P. flexuosa could explain the greater cytotoxicity on glial cells with respect to P. nigra that was not shown to contain these alkaloids
description Fil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Cholich, Luciana Andrea., et. al., 2021. Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae). Revista de Biología Tropical. San José: Universidad de Costa Rica, vol. 69, no. 1, p. 197-2060. E-ISSN 2215-2075.
0034-7744
http://repositorio.unne.edu.ar/handle/123456789/53153
identifier_str_mv Cholich, Luciana Andrea., et. al., 2021. Characterization and cytotoxic activity on glial cells of alkaloid-enriched extracts from pods of the plants prosopis flexuosa and prosopis Nigra (Fabaceae). Revista de Biología Tropical. San José: Universidad de Costa Rica, vol. 69, no. 1, p. 197-2060. E-ISSN 2215-2075.
0034-7744
url http://repositorio.unne.edu.ar/handle/123456789/53153
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 197-206
application/pdf
dc.publisher.none.fl_str_mv Universidad de Costa Rica
publisher.none.fl_str_mv Universidad de Costa Rica
dc.source.none.fl_str_mv Revista de Biología Tropical, 2021, vol. 69, no. 1, p. 197-206.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1844621669048516609
score 12.559606