In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants

Autores
Dolce, Natalia Raquel; Faloci, Mirta Mabel; González, Ana María
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Faloci, Mirta Mabel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Arachis glabrata Benth (perennial peanut) is a rhizomatous legume with high forage value and great potential for soil conservation as well as it displays valuable plant genetic resources for the cultivated edible peanut improvement. In this study, we developed for the first time successful protocols for micropropagation and cryopreservation of A. glabrata. First fully expanded leaflets from greenhousegrowing plants were efficiently established in vitro (93%) and displayed high frequency of bud induction (58%) on MS medium with 6 mg L−1 1-fenil-3-(1,2,3-tiadiazol-5-il)urea [TDZ]. Whole plant regeneration was achieved via direct organogenesis by transferring the induced buds to MS media. Immature unexpanded leaves from micropropagated plants were effectively cryopreserved by using the dropletvitrification technique. Maximum survival (~ 70%) and further regeneration (60–67%) were obtained by preconditioning immature leaves on semisolid MS with 0.3 M sucrose (1 d), exposing to loading solution consisting of 0.4 M sucrose plus 2 M glycerol (30 min) followed by glycerol-sucrose plant vitrification solution PVS3 (150 min in ice), and direct plunging into liquid nitrogen in droplets of PVS3 deposited on cryoplates. Tissues were rewarmed by plunging the aluminum foils directly in liquid MS enriched with 1.2 M sucrose (15 min) at room temperature. Growth recovery and plant regeneration were efficiently achieved via shoot organogenesis, and somatic embryogenesis by culturing cryostored explants on MS added with 6 mg L−1 TDZ. Genetic stability of plants derived from cryopreserved leaves was confirmed by random amplified polymorphic DNA markers. The protocols established in this study have great potential for rapid multiplication and conservation of selected A. glabrata genotypes.
Fuente
In Vitro Cellular & Developmental Biology-Plant, 2017, vol. 54, p.133-144.
Materia
Shoot organogenesis
Somatic embryogenesis
Thidiazuron
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/27531

id RIUNNE_b80e94a4a232cff424d4acf0e9cc8e5a
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/27531
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explantsDolce, Natalia RaquelFaloci, Mirta MabelGonzález, Ana MaríaShoot organogenesisSomatic embryogenesisThidiazuronFil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Faloci, Mirta Mabel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.Arachis glabrata Benth (perennial peanut) is a rhizomatous legume with high forage value and great potential for soil conservation as well as it displays valuable plant genetic resources for the cultivated edible peanut improvement. In this study, we developed for the first time successful protocols for micropropagation and cryopreservation of A. glabrata. First fully expanded leaflets from greenhousegrowing plants were efficiently established in vitro (93%) and displayed high frequency of bud induction (58%) on MS medium with 6 mg L−1 1-fenil-3-(1,2,3-tiadiazol-5-il)urea [TDZ]. Whole plant regeneration was achieved via direct organogenesis by transferring the induced buds to MS media. Immature unexpanded leaves from micropropagated plants were effectively cryopreserved by using the dropletvitrification technique. Maximum survival (~ 70%) and further regeneration (60–67%) were obtained by preconditioning immature leaves on semisolid MS with 0.3 M sucrose (1 d), exposing to loading solution consisting of 0.4 M sucrose plus 2 M glycerol (30 min) followed by glycerol-sucrose plant vitrification solution PVS3 (150 min in ice), and direct plunging into liquid nitrogen in droplets of PVS3 deposited on cryoplates. Tissues were rewarmed by plunging the aluminum foils directly in liquid MS enriched with 1.2 M sucrose (15 min) at room temperature. Growth recovery and plant regeneration were efficiently achieved via shoot organogenesis, and somatic embryogenesis by culturing cryostored explants on MS added with 6 mg L−1 TDZ. Genetic stability of plants derived from cryopreserved leaves was confirmed by random amplified polymorphic DNA markers. The protocols established in this study have great potential for rapid multiplication and conservation of selected A. glabrata genotypes.Springer2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp.133-144application/pdfDolce, Natalia Raquel, Faloci, Mirta Mabel y González, A. M., 2017. In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants. In Vitro Cellular & Developmental Biology-Plant. New York: Springer, vol. 54, p.133–144. ISSN 1054-5476.http://repositorio.unne.edu.ar/handle/123456789/27531In Vitro Cellular & Developmental Biology-Plant, 2017, vol. 54, p.133-144.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://doi.org/10.1007/s11627-017-9865-yinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:30:40Zoai:repositorio.unne.edu.ar:123456789/27531instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:40.469Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
title In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
spellingShingle In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
Dolce, Natalia Raquel
Shoot organogenesis
Somatic embryogenesis
Thidiazuron
title_short In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
title_full In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
title_fullStr In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
title_full_unstemmed In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
title_sort In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants
dc.creator.none.fl_str_mv Dolce, Natalia Raquel
Faloci, Mirta Mabel
González, Ana María
author Dolce, Natalia Raquel
author_facet Dolce, Natalia Raquel
Faloci, Mirta Mabel
González, Ana María
author_role author
author2 Faloci, Mirta Mabel
González, Ana María
author2_role author
author
dc.subject.none.fl_str_mv Shoot organogenesis
Somatic embryogenesis
Thidiazuron
topic Shoot organogenesis
Somatic embryogenesis
Thidiazuron
dc.description.none.fl_txt_mv Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Faloci, Mirta Mabel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: González, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Arachis glabrata Benth (perennial peanut) is a rhizomatous legume with high forage value and great potential for soil conservation as well as it displays valuable plant genetic resources for the cultivated edible peanut improvement. In this study, we developed for the first time successful protocols for micropropagation and cryopreservation of A. glabrata. First fully expanded leaflets from greenhousegrowing plants were efficiently established in vitro (93%) and displayed high frequency of bud induction (58%) on MS medium with 6 mg L−1 1-fenil-3-(1,2,3-tiadiazol-5-il)urea [TDZ]. Whole plant regeneration was achieved via direct organogenesis by transferring the induced buds to MS media. Immature unexpanded leaves from micropropagated plants were effectively cryopreserved by using the dropletvitrification technique. Maximum survival (~ 70%) and further regeneration (60–67%) were obtained by preconditioning immature leaves on semisolid MS with 0.3 M sucrose (1 d), exposing to loading solution consisting of 0.4 M sucrose plus 2 M glycerol (30 min) followed by glycerol-sucrose plant vitrification solution PVS3 (150 min in ice), and direct plunging into liquid nitrogen in droplets of PVS3 deposited on cryoplates. Tissues were rewarmed by plunging the aluminum foils directly in liquid MS enriched with 1.2 M sucrose (15 min) at room temperature. Growth recovery and plant regeneration were efficiently achieved via shoot organogenesis, and somatic embryogenesis by culturing cryostored explants on MS added with 6 mg L−1 TDZ. Genetic stability of plants derived from cryopreserved leaves was confirmed by random amplified polymorphic DNA markers. The protocols established in this study have great potential for rapid multiplication and conservation of selected A. glabrata genotypes.
description Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Dolce, Natalia Raquel, Faloci, Mirta Mabel y González, A. M., 2017. In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants. In Vitro Cellular & Developmental Biology-Plant. New York: Springer, vol. 54, p.133–144. ISSN 1054-5476.
http://repositorio.unne.edu.ar/handle/123456789/27531
identifier_str_mv Dolce, Natalia Raquel, Faloci, Mirta Mabel y González, A. M., 2017. In vitro plant regeneration and cryopreservation of Arachis glabrata (Fabaceae) using leaflet explants. In Vitro Cellular & Developmental Biology-Plant. New York: Springer, vol. 54, p.133–144. ISSN 1054-5476.
url http://repositorio.unne.edu.ar/handle/123456789/27531
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.1007/s11627-017-9865-y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p.133-144
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv In Vitro Cellular & Developmental Biology-Plant, 2017, vol. 54, p.133-144.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1844621695080464384
score 12.559606