In vitro propagation and germplasm conservation of wild orchids from South America

• Autores: Dolce, Natalia Raquel; Medina, Ricardo Daniel; Terada, Graciela Raquel; González Arnao, María Teresa; Flachsland, Eduardo Alberto
• Año de publicación: 2020
• Idioma: inglés
• Tipo de recurso: parte de libro
• Materia: Orchid biodiversity; Seed germination; Somatic embryogenesis; Shoot organogenesis; Plant regeneration; Seeds storage; Pollen storage; Cryopreservation
• Institución: Universidad Nacional del Nordeste

Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Medina, Ricardo Daniel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Terada, Graciela Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Terada, Graciela Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Flachsland, Alberto Eduardo. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Flachsland, Alberto Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.