Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture

Autores
Fontana, María Laura; Mroginski, Luis Amado; Rey, Hebe Yolanda
Año de publicación
2009
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Fontana, María Laura. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Faculta de Ciencias Agrarias; Argentina.
Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Faculta de Ciencias Agrarias; Argentina.
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: αaphthalenacetic acid, in- dole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 μM α-naphthalenacetic acid, 13.95 μM kinetin and 13.32 μM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 μM α-naphthalenacetic acid.
Fuente
Biocell, 2009, vol. 33, no. 3, p. 179-186.
Materia
In vitro cultivation
Leaflet explants
Micropropagation
Thidiazuron
Wild groundnut
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/48613
Acceder
id RIUNNE_67c54e5af983f1c0c9453c7af609ba37
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/48613
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf cultureFontana, María LauraMroginski, Luis AmadoRey, Hebe YolandaIn vitro cultivationLeaflet explantsMicropropagationThidiazuronWild groundnutFil: Fontana, María Laura. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Faculta de Ciencias Agrarias; Argentina.Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Faculta de Ciencias Agrarias; Argentina.With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: αaphthalenacetic acid, in- dole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 μM α-naphthalenacetic acid, 13.95 μM kinetin and 13.32 μM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 μM α-naphthalenacetic acid.Sociedad Latinoamericana de Microscopia Electrónica2009-11-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfFontana, María Laura, Mroginski Luis Amado y Rey, Hebe Yolanda, 2009. Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture. Biocell. Mendoza: Sociedad Latinoamericana de Microscopia Electrónica, vol. 33, no. 3, p. 179-186. e-ISSN 1667-5746.0327-9545http://repositorio.unne.edu.ar/handle/123456789/48613Biocell, 2009, vol. 33, no. 3, p. 179-186.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordestespahttps://www.techscience.com/biocell/v33n3/37769/pdfinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-10-23T11:18:10Zoai:repositorio.unne.edu.ar:123456789/48613instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-10-23 11:18:10.473Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture
title Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture
spellingShingle Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture
Fontana, María Laura
In vitro cultivation
Leaflet explants
Micropropagation
Thidiazuron
Wild groundnut
title_short Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture
title_full Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture
title_fullStr Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture
title_full_unstemmed Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture
title_sort Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture
dc.creator.none.fl_str_mv Fontana, María Laura
Mroginski, Luis Amado
Rey, Hebe Yolanda
author Fontana, María Laura
author_facet Fontana, María Laura
Mroginski, Luis Amado
Rey, Hebe Yolanda
author_role author
author2 Mroginski, Luis Amado
Rey, Hebe Yolanda
author2_role author
author
dc.subject.none.fl_str_mv In vitro cultivation
Leaflet explants
Micropropagation
Thidiazuron
Wild groundnut
topic In vitro cultivation
Leaflet explants
Micropropagation
Thidiazuron
Wild groundnut
dc.description.none.fl_txt_mv Fil: Fontana, María Laura. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Faculta de Ciencias Agrarias; Argentina.
Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Faculta de Ciencias Agrarias; Argentina.
With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: αaphthalenacetic acid, in- dole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in Arachis villosa accession L97. A thidiazuron lacking media (only 0.54 μM α-naphthalenacetic acid, 13.95 μM kinetin and 13.32 μM 6-benzylaminopurine were added) promoted the elongation of the regenerated buds. Adventitious rooting was achieved 90 days after the isolated shoots were transferred to a rooting medium containing 0.54 μM α-naphthalenacetic acid.
description Fil: Fontana, María Laura. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
publishDate 2009
dc.date.none.fl_str_mv 2009-11-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Fontana, María Laura, Mroginski Luis Amado y Rey, Hebe Yolanda, 2009. Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture. Biocell. Mendoza: Sociedad Latinoamericana de Microscopia Electrónica, vol. 33, no. 3, p. 179-186. e-ISSN 1667-5746.
0327-9545
http://repositorio.unne.edu.ar/handle/123456789/48613
identifier_str_mv Fontana, María Laura, Mroginski Luis Amado y Rey, Hebe Yolanda, 2009. Organogenesis and plant regeneration of arachis villosa benth. (leguminosae) through leaf culture. Biocell. Mendoza: Sociedad Latinoamericana de Microscopia Electrónica, vol. 33, no. 3, p. 179-186. e-ISSN 1667-5746.
0327-9545
url http://repositorio.unne.edu.ar/handle/123456789/48613
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://www.techscience.com/biocell/v33n3/37769/pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Latinoamericana de Microscopia Electrónica
publisher.none.fl_str_mv Sociedad Latinoamericana de Microscopia Electrónica
dc.source.none.fl_str_mv Biocell, 2009, vol. 33, no. 3, p. 179-186.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1846787776010256384
score 12.982451

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