An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis

Autores
Sharry, Sandra Elizabeth; Cabrera Ponce, José Luis; Herrera Estrella, Luis; Rangel Cano, Rosa María; Lede, Silvia; Abedini, Walter
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry (Melia azedarach L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include: a) Embryogenic callus induction with no addition of 2, 4-D in the culture media; b) Somatic embryos differentiation was achieved by using high concentration of cytokinins (BAP 10 mg/L) and adenine; c) 100% conversion of somatic embryos to plants was practically obtained and 100% of plants survived under greenhouse conditions; d) Addition of putrescine improved somatic embryos germination. The amount of somatic embryos produced by the pathway of indirect somatic embryogenesis was 447 per gram of fresh weight callus. Regenerated plants were phenotypically normal. The developed protocol established the potential to produce plantlets from cotyledon explants through somatic embryogenesis. It also presents itself as a highly efficient method for mass clonal propagation and conservation of Melia azedarach.
Centro Experimental de Propagación Vegetativa
Materia
Ciencias Agrarias
Melia azedarach L.
Somatic embryogenesis system
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/108589
Acceder
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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesisSharry, Sandra ElizabethCabrera Ponce, José LuisHerrera Estrella, LuisRangel Cano, Rosa MaríaLede, SilviaAbedini, WalterCiencias AgrariasMelia azedarach L.Somatic embryogenesis systemA highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry (Melia azedarach L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include: a) Embryogenic callus induction with no addition of 2, 4-D in the culture media; b) Somatic embryos differentiation was achieved by using high concentration of cytokinins (BAP 10 mg/L) and adenine; c) 100% conversion of somatic embryos to plants was practically obtained and 100% of plants survived under greenhouse conditions; d) Addition of putrescine improved somatic embryos germination. The amount of somatic embryos produced by the pathway of indirect somatic embryogenesis was 447 per gram of fresh weight callus. Regenerated plants were phenotypically normal. The developed protocol established the potential to produce plantlets from cotyledon explants through somatic embryogenesis. It also presents itself as a highly efficient method for mass clonal propagation and conservation of Melia azedarach.Centro Experimental de Propagación Vegetativa2006info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf187-194http://sedici.unlp.edu.ar/handle/10915/108589enginfo:eu-repo/semantics/altIdentifier/issn/0717-3458info:eu-repo/semantics/altIdentifier/doi/10.2225/vol9-issue3-fulltext-13info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:24:36Zoai:sedici.unlp.edu.ar:10915/108589Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:24:37.284SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
title An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
spellingShingle An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
Sharry, Sandra Elizabeth
Ciencias Agrarias
Melia azedarach L.
Somatic embryogenesis system
title_short An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
title_full An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
title_fullStr An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
title_full_unstemmed An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
title_sort An alternative pathway for plant in vitro regeneration of chinaberry -tree Melia azedarach L. derived from the induction of somatic embryogenesis
dc.creator.none.fl_str_mv Sharry, Sandra Elizabeth
Cabrera Ponce, José Luis
Herrera Estrella, Luis
Rangel Cano, Rosa María
Lede, Silvia
Abedini, Walter
author Sharry, Sandra Elizabeth
author_facet Sharry, Sandra Elizabeth
Cabrera Ponce, José Luis
Herrera Estrella, Luis
Rangel Cano, Rosa María
Lede, Silvia
Abedini, Walter
author_role author
author2 Cabrera Ponce, José Luis
Herrera Estrella, Luis
Rangel Cano, Rosa María
Lede, Silvia
Abedini, Walter
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Agrarias
Melia azedarach L.
Somatic embryogenesis system
topic Ciencias Agrarias
Melia azedarach L.
Somatic embryogenesis system
dc.description.none.fl_txt_mv A highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry (Melia azedarach L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include: a) Embryogenic callus induction with no addition of 2, 4-D in the culture media; b) Somatic embryos differentiation was achieved by using high concentration of cytokinins (BAP 10 mg/L) and adenine; c) 100% conversion of somatic embryos to plants was practically obtained and 100% of plants survived under greenhouse conditions; d) Addition of putrescine improved somatic embryos germination. The amount of somatic embryos produced by the pathway of indirect somatic embryogenesis was 447 per gram of fresh weight callus. Regenerated plants were phenotypically normal. The developed protocol established the potential to produce plantlets from cotyledon explants through somatic embryogenesis. It also presents itself as a highly efficient method for mass clonal propagation and conservation of Melia azedarach.
Centro Experimental de Propagación Vegetativa
description A highly efficient somatic embryogenesis system and subsequent plant regeneration of chinaberry (Melia azedarach L.) was developed. Plants were regenerated from indirect somatic embryogenesis induction. Novel features of this improved protocol, include: a) Embryogenic callus induction with no addition of 2, 4-D in the culture media; b) Somatic embryos differentiation was achieved by using high concentration of cytokinins (BAP 10 mg/L) and adenine; c) 100% conversion of somatic embryos to plants was practically obtained and 100% of plants survived under greenhouse conditions; d) Addition of putrescine improved somatic embryos germination. The amount of somatic embryos produced by the pathway of indirect somatic embryogenesis was 447 per gram of fresh weight callus. Regenerated plants were phenotypically normal. The developed protocol established the potential to produce plantlets from cotyledon explants through somatic embryogenesis. It also presents itself as a highly efficient method for mass clonal propagation and conservation of Melia azedarach.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/108589
url http://sedici.unlp.edu.ar/handle/10915/108589
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0717-3458
info:eu-repo/semantics/altIdentifier/doi/10.2225/vol9-issue3-fulltext-13
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
187-194
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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