Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities

Autores
Guidoli, Marcos Gabriel; Santinón, Juan José; Pasteri, Sergio Enrique; Sánchez, Sebastián; Nader Macías, María Elena Fátima
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Piaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. The high demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and the incidence of diseases had intensified this deficit. Probiotics are proven to increase survival and growth rates in aquaculture. The first step in the design of a putative probiotic is the selection of adequate microorganisms to boost fish health. Lactic Acid Bacteria and Bacillus are generally accepted as probiotics in aquaculture. In this work we obtained 522 isolates from different anatomical parts of P. mesopotamicus, with a total of 30 lactic acid bacteria (LAB) and 8 Bacillus strains submitted for the evaluation of their beneficial properties. Only eight of these strains were selected for further “in vivo” assays based on following criteria: production of inhibitory metabolites and biosurfactants, screening of antagonistic activity against foodborne and pathogenic microorganisms, ability to emulsify polar solvents and compatibility between strains. All selected microorganisms were identified by sequencing 16S RNA gene. Pure cultures of all strains herein studied are preserved in Centro de Referencia de Lactobacilos (CERELA-CONICET) and Laboratorio de Sanidad Animal of the Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA) culture collections.
Fil: Guidoli, Marcos Gabriel. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina.
Fil: Santinón, Juan José. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina.
Fil: Sánchez, Sebastián. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina.
Fil: Nader Macías, María Elena Fátima. Universidad Nacional del Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina.
Fuente
Journal Bioprocess Biotech, 2015, vol. 5, no. 9, p. 1-10.
Materia
Piaractus mesopotamicus
Beneficial bacterial strains
Probiotics
Aquaculture
Lactic acid bacteria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/1542
Acceder
id RIUNNE_9129aed202f406a49aac047e429d7697
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network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activitiesGuidoli, Marcos GabrielSantinón, Juan JoséPasteri, Sergio EnriqueSánchez, SebastiánNader Macías, María Elena FátimaPiaractus mesopotamicusBeneficial bacterial strainsProbioticsAquacultureLactic acid bacteriaPiaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. The high demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and the incidence of diseases had intensified this deficit. Probiotics are proven to increase survival and growth rates in aquaculture. The first step in the design of a putative probiotic is the selection of adequate microorganisms to boost fish health. Lactic Acid Bacteria and Bacillus are generally accepted as probiotics in aquaculture. In this work we obtained 522 isolates from different anatomical parts of P. mesopotamicus, with a total of 30 lactic acid bacteria (LAB) and 8 Bacillus strains submitted for the evaluation of their beneficial properties. Only eight of these strains were selected for further “in vivo” assays based on following criteria: production of inhibitory metabolites and biosurfactants, screening of antagonistic activity against foodborne and pathogenic microorganisms, ability to emulsify polar solvents and compatibility between strains. All selected microorganisms were identified by sequencing 16S RNA gene. Pure cultures of all strains herein studied are preserved in Centro de Referencia de Lactobacilos (CERELA-CONICET) and Laboratorio de Sanidad Animal of the Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA) culture collections.Fil: Guidoli, Marcos Gabriel. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina.Fil: Santinón, Juan José. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina.Fil: Sánchez, Sebastián. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina.Fil: Nader Macías, María Elena Fátima. Universidad Nacional del Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina.Elsevier2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 1-10application/pdfGuidoli, Marcos Gabriel, et al., 2015. Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities. Journal Bioprocess Biotech. New York: Elsevier, vol. 5, no. 9, p. 1-10. ISSN 2155-9821.http://repositorio.unne.edu.ar/handle/123456789/1542Journal Bioprocess Biotech, 2015, vol. 5, no. 9, p. 1-10.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-04T11:14:11Zoai:repositorio.unne.edu.ar:123456789/1542instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-04 11:14:11.955Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
title Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
spellingShingle Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
Guidoli, Marcos Gabriel
Piaractus mesopotamicus
Beneficial bacterial strains
Probiotics
Aquaculture
Lactic acid bacteria
title_short Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
title_full Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
title_fullStr Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
title_full_unstemmed Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
title_sort Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities
dc.creator.none.fl_str_mv Guidoli, Marcos Gabriel
Santinón, Juan José
Pasteri, Sergio Enrique
Sánchez, Sebastián
Nader Macías, María Elena Fátima
author Guidoli, Marcos Gabriel
author_facet Guidoli, Marcos Gabriel
Santinón, Juan José
Pasteri, Sergio Enrique
Sánchez, Sebastián
Nader Macías, María Elena Fátima
author_role author
author2 Santinón, Juan José
Pasteri, Sergio Enrique
Sánchez, Sebastián
Nader Macías, María Elena Fátima
author2_role author
author
author
author
dc.subject.none.fl_str_mv Piaractus mesopotamicus
Beneficial bacterial strains
Probiotics
Aquaculture
Lactic acid bacteria
topic Piaractus mesopotamicus
Beneficial bacterial strains
Probiotics
Aquaculture
Lactic acid bacteria
dc.description.none.fl_txt_mv Piaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. The high demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and the incidence of diseases had intensified this deficit. Probiotics are proven to increase survival and growth rates in aquaculture. The first step in the design of a putative probiotic is the selection of adequate microorganisms to boost fish health. Lactic Acid Bacteria and Bacillus are generally accepted as probiotics in aquaculture. In this work we obtained 522 isolates from different anatomical parts of P. mesopotamicus, with a total of 30 lactic acid bacteria (LAB) and 8 Bacillus strains submitted for the evaluation of their beneficial properties. Only eight of these strains were selected for further “in vivo” assays based on following criteria: production of inhibitory metabolites and biosurfactants, screening of antagonistic activity against foodborne and pathogenic microorganisms, ability to emulsify polar solvents and compatibility between strains. All selected microorganisms were identified by sequencing 16S RNA gene. Pure cultures of all strains herein studied are preserved in Centro de Referencia de Lactobacilos (CERELA-CONICET) and Laboratorio de Sanidad Animal of the Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA) culture collections.
Fil: Guidoli, Marcos Gabriel. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina.
Fil: Santinón, Juan José. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina.
Fil: Sánchez, Sebastián. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina.
Fil: Nader Macías, María Elena Fátima. Universidad Nacional del Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina.
description Piaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. The high demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and the incidence of diseases had intensified this deficit. Probiotics are proven to increase survival and growth rates in aquaculture. The first step in the design of a putative probiotic is the selection of adequate microorganisms to boost fish health. Lactic Acid Bacteria and Bacillus are generally accepted as probiotics in aquaculture. In this work we obtained 522 isolates from different anatomical parts of P. mesopotamicus, with a total of 30 lactic acid bacteria (LAB) and 8 Bacillus strains submitted for the evaluation of their beneficial properties. Only eight of these strains were selected for further “in vivo” assays based on following criteria: production of inhibitory metabolites and biosurfactants, screening of antagonistic activity against foodborne and pathogenic microorganisms, ability to emulsify polar solvents and compatibility between strains. All selected microorganisms were identified by sequencing 16S RNA gene. Pure cultures of all strains herein studied are preserved in Centro de Referencia de Lactobacilos (CERELA-CONICET) and Laboratorio de Sanidad Animal of the Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA) culture collections.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Guidoli, Marcos Gabriel, et al., 2015. Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities. Journal Bioprocess Biotech. New York: Elsevier, vol. 5, no. 9, p. 1-10. ISSN 2155-9821.
http://repositorio.unne.edu.ar/handle/123456789/1542
identifier_str_mv Guidoli, Marcos Gabriel, et al., 2015. Isolation and selection of potentially beneficial autochthonous bacteria for Piaractus mesopotamicus aquaculture activities. Journal Bioprocess Biotech. New York: Elsevier, vol. 5, no. 9, p. 1-10. ISSN 2155-9821.
url http://repositorio.unne.edu.ar/handle/123456789/1542
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 1-10
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal Bioprocess Biotech, 2015, vol. 5, no. 9, p. 1-10.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 12.623145

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