Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities

Autores
Guidoli, Marcos Gabriel; Santinón, Juan José; Pasteris, Sergio Enrique; Sánchez, Sebastián; Nader, Maria Elena Fatima
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Piaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. Thehigh demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and theincidence of diseases had intensified this deficit. Probiotics are proven to increase survival and growth rates in aquaculture. Thefirst step in the design of a putative probiotic is the selection of adequate microorganisms to boost fish health. Lactic Acid Bacteriaand Bacillus are generally accepted as probiotics in aquaculture. In this work we obtained 522 isolates from different anatomicalparts of P. mesopotamicus, with a total of 30 lactic acid bacteria (LAB) and 8 Bacillus strains submitted for the evaluation of theirbeneficial properties. Only eight of these strains were selected for further "in vivo" assays based on following criteria: production ofinhibitory metabolites and biosurfactants, screening of antagonistic activity against foodborne and pathogenic microorganisms, abilityto emulsify polar solvents and compatibility between strains. All selected microorganisms were identified by sequencing 16S RNAgene. Pure cultures of all strains herein studied are preserved in Centro de Referencia de Lactobacilos (CERELA-CONICET) andLaboratorio de Sanidad Animal of the Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria(INTA) culture collections
Fil: Guidoli, Marcos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina
Fil: Santinón, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina
Fil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Sánchez, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Materia
PIARACTUS MESOPATAMICUS
BENEFICIAL BACTERIAL STRAINS
PROBIOTICS
AQUACULTURE
LACTIC ACID BACTERIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/127208
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture ActivitiesGuidoli, Marcos GabrielSantinón, Juan JoséPasteris, Sergio EnriqueSánchez, SebastiánNader, Maria Elena FatimaPIARACTUS MESOPATAMICUSBENEFICIAL BACTERIAL STRAINSPROBIOTICSAQUACULTURELACTIC ACID BACTERIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Piaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. Thehigh demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and theincidence of diseases had intensified this deficit. Probiotics are proven to increase survival and growth rates in aquaculture. Thefirst step in the design of a putative probiotic is the selection of adequate microorganisms to boost fish health. Lactic Acid Bacteriaand Bacillus are generally accepted as probiotics in aquaculture. In this work we obtained 522 isolates from different anatomicalparts of P. mesopotamicus, with a total of 30 lactic acid bacteria (LAB) and 8 Bacillus strains submitted for the evaluation of theirbeneficial properties. Only eight of these strains were selected for further "in vivo" assays based on following criteria: production ofinhibitory metabolites and biosurfactants, screening of antagonistic activity against foodborne and pathogenic microorganisms, abilityto emulsify polar solvents and compatibility between strains. All selected microorganisms were identified by sequencing 16S RNAgene. Pure cultures of all strains herein studied are preserved in Centro de Referencia de Lactobacilos (CERELA-CONICET) andLaboratorio de Sanidad Animal of the Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria(INTA) culture collectionsFil: Guidoli, Marcos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; ArgentinaFil: Santinón, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; ArgentinaFil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Sánchez, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaOMICS2015-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/127208Guidoli, Marcos Gabriel; Santinón, Juan José; Pasteris, Sergio Enrique; Sánchez, Sebastián; Nader, Maria Elena Fatima; Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities; OMICS; Journal of Bioprocessing and Biotechniques; 5; 9; 9-2015; 1-10; 10002542155-9821CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2155-9821.1000254info:eu-repo/semantics/altIdentifier/url/https://www.hilarispublisher.com/open-access/isolation-and-selection-of-potentially-beneficial-autochthonous-bacteria-for-piaractus-mesopotamicus-aquaculture-activities-2155-9821-1000254.pdfinfo:eu-repo/semantics/altIdentifier/url/http://www.omicsonline.org/open-access/isolation-and-selection-of-potentially-beneficial-autochthonous-bacteria-for-piaractus-mesopotamicus-aquaculture-activities-2155-9821-1000254.php?aid=61083info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:39Zoai:ri.conicet.gov.ar:11336/127208instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:39.757CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities
title Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities
spellingShingle Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities
Guidoli, Marcos Gabriel
PIARACTUS MESOPATAMICUS
BENEFICIAL BACTERIAL STRAINS
PROBIOTICS
AQUACULTURE
LACTIC ACID BACTERIA
title_short Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities
title_full Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities
title_fullStr Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities
title_full_unstemmed Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities
title_sort Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities
dc.creator.none.fl_str_mv Guidoli, Marcos Gabriel
Santinón, Juan José
Pasteris, Sergio Enrique
Sánchez, Sebastián
Nader, Maria Elena Fatima
author Guidoli, Marcos Gabriel
author_facet Guidoli, Marcos Gabriel
Santinón, Juan José
Pasteris, Sergio Enrique
Sánchez, Sebastián
Nader, Maria Elena Fatima
author_role author
author2 Santinón, Juan José
Pasteris, Sergio Enrique
Sánchez, Sebastián
Nader, Maria Elena Fatima
author2_role author
author
author
author
dc.subject.none.fl_str_mv PIARACTUS MESOPATAMICUS
BENEFICIAL BACTERIAL STRAINS
PROBIOTICS
AQUACULTURE
LACTIC ACID BACTERIA
topic PIARACTUS MESOPATAMICUS
BENEFICIAL BACTERIAL STRAINS
PROBIOTICS
AQUACULTURE
LACTIC ACID BACTERIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Piaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. Thehigh demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and theincidence of diseases had intensified this deficit. Probiotics are proven to increase survival and growth rates in aquaculture. Thefirst step in the design of a putative probiotic is the selection of adequate microorganisms to boost fish health. Lactic Acid Bacteriaand Bacillus are generally accepted as probiotics in aquaculture. In this work we obtained 522 isolates from different anatomicalparts of P. mesopotamicus, with a total of 30 lactic acid bacteria (LAB) and 8 Bacillus strains submitted for the evaluation of theirbeneficial properties. Only eight of these strains were selected for further "in vivo" assays based on following criteria: production ofinhibitory metabolites and biosurfactants, screening of antagonistic activity against foodborne and pathogenic microorganisms, abilityto emulsify polar solvents and compatibility between strains. All selected microorganisms were identified by sequencing 16S RNAgene. Pure cultures of all strains herein studied are preserved in Centro de Referencia de Lactobacilos (CERELA-CONICET) andLaboratorio de Sanidad Animal of the Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria(INTA) culture collections
Fil: Guidoli, Marcos Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina
Fil: Santinón, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina
Fil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Sánchez, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias. Instituto de Ictiología del Nordeste; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
description Piaractus mesopotamicus is the most produced specie in Argentinian fish culture with an annual production of 2,017 tons. Thehigh demands of specimens generated a deficit of larvae, juveniles and sexually mature adults. The high stress conditions and theincidence of diseases had intensified this deficit. Probiotics are proven to increase survival and growth rates in aquaculture. Thefirst step in the design of a putative probiotic is the selection of adequate microorganisms to boost fish health. Lactic Acid Bacteriaand Bacillus are generally accepted as probiotics in aquaculture. In this work we obtained 522 isolates from different anatomicalparts of P. mesopotamicus, with a total of 30 lactic acid bacteria (LAB) and 8 Bacillus strains submitted for the evaluation of theirbeneficial properties. Only eight of these strains were selected for further "in vivo" assays based on following criteria: production ofinhibitory metabolites and biosurfactants, screening of antagonistic activity against foodborne and pathogenic microorganisms, abilityto emulsify polar solvents and compatibility between strains. All selected microorganisms were identified by sequencing 16S RNAgene. Pure cultures of all strains herein studied are preserved in Centro de Referencia de Lactobacilos (CERELA-CONICET) andLaboratorio de Sanidad Animal of the Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria(INTA) culture collections
publishDate 2015
dc.date.none.fl_str_mv 2015-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/127208
Guidoli, Marcos Gabriel; Santinón, Juan José; Pasteris, Sergio Enrique; Sánchez, Sebastián; Nader, Maria Elena Fatima; Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities; OMICS; Journal of Bioprocessing and Biotechniques; 5; 9; 9-2015; 1-10; 1000254
2155-9821
CONICET Digital
CONICET
url http://hdl.handle.net/11336/127208
identifier_str_mv Guidoli, Marcos Gabriel; Santinón, Juan José; Pasteris, Sergio Enrique; Sánchez, Sebastián; Nader, Maria Elena Fatima; Isolation and Selection of Potentially Beneficial Autochthonous Bacteria for Piaractus mesopotamicus Aquaculture Activities; OMICS; Journal of Bioprocessing and Biotechniques; 5; 9; 9-2015; 1-10; 1000254
2155-9821
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2155-9821.1000254
info:eu-repo/semantics/altIdentifier/url/https://www.hilarispublisher.com/open-access/isolation-and-selection-of-potentially-beneficial-autochthonous-bacteria-for-piaractus-mesopotamicus-aquaculture-activities-2155-9821-1000254.pdf
info:eu-repo/semantics/altIdentifier/url/http://www.omicsonline.org/open-access/isolation-and-selection-of-potentially-beneficial-autochthonous-bacteria-for-piaractus-mesopotamicus-aquaculture-activities-2155-9821-1000254.php?aid=61083
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS
publisher.none.fl_str_mv OMICS
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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