The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom

Autores
Ricciardi Verrastro, Bárbara Verónica; Torres, Ana María; Ricciardi, Gabriela Ana Leticia; Teibler, Gladys Pamela; Maruñak, Silvana Licia; Barnaba, Chiara; Larcher, Roberto; Nicolini, Giorgio; Dellacassa, Eduardo Santiago
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Ricciardi Verrastro, Bárbara Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Torres, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Ricciardi, Gabriela Ana Leticia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Veterinaria; Argentina.
Fil: Maruñak, Silvana Licia. Universidad Nacional del Nordeste. Facultad de Veterinaria; Argentina.
Fil: Barnaba, Chiara. Universidad de la República. Facultad de Química; Uruguay.
Fil: Larcher, Roberto. Universidad de la República. Facultad de Química; Uruguay.
Fil: Nicolini, Giorgio. Universidad de la República. Facultad de Química; Uruguay.
Fil: Dellacassa, Eduardo Santiago. Fondazione Edmund Mach di San Michele all'Adige. Centro Trasferimento Tecnologico; Italia.
Ethnopharmacological relevance: ophidian accidents are a serious public health problem in Argentina; the Bothrops species is responsible for 97% of these accidents, and in particular, B. diporus is responsible for 80% of them. In the northeast of the country (Corrientes Provinces), Cissampelos pareira L. (Menispermaceae) is commonly used against the venom of B. diporus; its use is described in almost all ethnobotanical literature from countries where the plant grows. Aim of the study: in this study, the in vitro and in vivo antivenom activities of C. pareira extracts were evaluated against B. diporus venom, with a particular focus on the local effects associated with envenoming. The seasonal influence on the chemical composition of the active extracts was also studied, in order determine the associated range of variability and its influence on the antivenom activity. Materials and Methods: this research was conducted using aerial parts (leaves, flowers, tender stems) and roots of Cissampelos pareira collected from two different phytogeographic regions of Corrientes (Argentina); Paso de la Patria and Lomas de Vallejos. In addition, to perform a seasonal analysis and to evaluate the metabolic stability, material was collected at three different growth stages. In vivo and in vitro anti-snake venom activities were tested, and a bio-guided chromatographic separation was performed in order to determine the active chemicals involved. The fractions obtained were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the chemical profile of the most active constituent was analyzed by ultra high-performance liquid chromatography coupled to quadrupole/high-resolution mass spectrometry (Q-Orbitrap). (UHPLC-MS). Results: the alcoholic extract was found to be the most active The bio-guided fractionation allowed selection one fraction to be analyzed by UHPLC-MS in order to identify the components responsible for the activities found; this identified five possible flavonoids. Conclusions: our studies of the activity of C. pareira against the venom of B. diporus have confirmed that this species possesses inhibitory effects in both in vitro and in vivo models. Moreover, the present data demonstrate that certain flavonoids may mitigate some of the venom-induced local tissue damage.
Fuente
Journal of Ethnopharmacology, 2018, vol. 212, p. 36-42.
Materia
Bothrops diporus
Ophidian accidents
Cissampelos pareira L.
Snake antivenom
UHPLC-MS (Q-Orbitrap)
Flavonoids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/30782

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repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venomRicciardi Verrastro, Bárbara VerónicaTorres, Ana MaríaRicciardi, Gabriela Ana LeticiaTeibler, Gladys PamelaMaruñak, Silvana LiciaBarnaba, ChiaraLarcher, RobertoNicolini, GiorgioDellacassa, Eduardo SantiagoBothrops diporusOphidian accidentsCissampelos pareira L.Snake antivenomUHPLC-MS (Q-Orbitrap)FlavonoidsFil: Ricciardi Verrastro, Bárbara Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.Fil: Torres, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.Fil: Ricciardi, Gabriela Ana Leticia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Veterinaria; Argentina.Fil: Maruñak, Silvana Licia. Universidad Nacional del Nordeste. Facultad de Veterinaria; Argentina.Fil: Barnaba, Chiara. Universidad de la República. Facultad de Química; Uruguay.Fil: Larcher, Roberto. Universidad de la República. Facultad de Química; Uruguay.Fil: Nicolini, Giorgio. Universidad de la República. Facultad de Química; Uruguay.Fil: Dellacassa, Eduardo Santiago. Fondazione Edmund Mach di San Michele all'Adige. Centro Trasferimento Tecnologico; Italia.Ethnopharmacological relevance: ophidian accidents are a serious public health problem in Argentina; the Bothrops species is responsible for 97% of these accidents, and in particular, B. diporus is responsible for 80% of them. In the northeast of the country (Corrientes Provinces), Cissampelos pareira L. (Menispermaceae) is commonly used against the venom of B. diporus; its use is described in almost all ethnobotanical literature from countries where the plant grows. Aim of the study: in this study, the in vitro and in vivo antivenom activities of C. pareira extracts were evaluated against B. diporus venom, with a particular focus on the local effects associated with envenoming. The seasonal influence on the chemical composition of the active extracts was also studied, in order determine the associated range of variability and its influence on the antivenom activity. Materials and Methods: this research was conducted using aerial parts (leaves, flowers, tender stems) and roots of Cissampelos pareira collected from two different phytogeographic regions of Corrientes (Argentina); Paso de la Patria and Lomas de Vallejos. In addition, to perform a seasonal analysis and to evaluate the metabolic stability, material was collected at three different growth stages. In vivo and in vitro anti-snake venom activities were tested, and a bio-guided chromatographic separation was performed in order to determine the active chemicals involved. The fractions obtained were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the chemical profile of the most active constituent was analyzed by ultra high-performance liquid chromatography coupled to quadrupole/high-resolution mass spectrometry (Q-Orbitrap). (UHPLC-MS). Results: the alcoholic extract was found to be the most active The bio-guided fractionation allowed selection one fraction to be analyzed by UHPLC-MS in order to identify the components responsible for the activities found; this identified five possible flavonoids. Conclusions: our studies of the activity of C. pareira against the venom of B. diporus have confirmed that this species possesses inhibitory effects in both in vitro and in vivo models. Moreover, the present data demonstrate that certain flavonoids may mitigate some of the venom-induced local tissue damage.Elsevier2018-02-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfRicciardi Verrastro, Bárbara Verónica, et al., 2018. The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom. Journal of Ethnopharmacology. Ámsterdam: Elsevier, vol. 212, p. 36-42. ISSN 0378-8741.0378-8741http://repositorio.unne.edu.ar/handle/123456789/30782Journal of Ethnopharmacology, 2018, vol. 212, p. 36-42.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttp://dx.doi.org/10.1016/j.jep.2017.09.015info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:30:29Zoai:repositorio.unne.edu.ar:123456789/30782instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:29.401Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom
title The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom
spellingShingle The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom
Ricciardi Verrastro, Bárbara Verónica
Bothrops diporus
Ophidian accidents
Cissampelos pareira L.
Snake antivenom
UHPLC-MS (Q-Orbitrap)
Flavonoids
title_short The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom
title_full The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom
title_fullStr The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom
title_full_unstemmed The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom
title_sort The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom
dc.creator.none.fl_str_mv Ricciardi Verrastro, Bárbara Verónica
Torres, Ana María
Ricciardi, Gabriela Ana Leticia
Teibler, Gladys Pamela
Maruñak, Silvana Licia
Barnaba, Chiara
Larcher, Roberto
Nicolini, Giorgio
Dellacassa, Eduardo Santiago
author Ricciardi Verrastro, Bárbara Verónica
author_facet Ricciardi Verrastro, Bárbara Verónica
Torres, Ana María
Ricciardi, Gabriela Ana Leticia
Teibler, Gladys Pamela
Maruñak, Silvana Licia
Barnaba, Chiara
Larcher, Roberto
Nicolini, Giorgio
Dellacassa, Eduardo Santiago
author_role author
author2 Torres, Ana María
Ricciardi, Gabriela Ana Leticia
Teibler, Gladys Pamela
Maruñak, Silvana Licia
Barnaba, Chiara
Larcher, Roberto
Nicolini, Giorgio
Dellacassa, Eduardo Santiago
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bothrops diporus
Ophidian accidents
Cissampelos pareira L.
Snake antivenom
UHPLC-MS (Q-Orbitrap)
Flavonoids
topic Bothrops diporus
Ophidian accidents
Cissampelos pareira L.
Snake antivenom
UHPLC-MS (Q-Orbitrap)
Flavonoids
dc.description.none.fl_txt_mv Fil: Ricciardi Verrastro, Bárbara Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Torres, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Ricciardi, Gabriela Ana Leticia. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Veterinaria; Argentina.
Fil: Maruñak, Silvana Licia. Universidad Nacional del Nordeste. Facultad de Veterinaria; Argentina.
Fil: Barnaba, Chiara. Universidad de la República. Facultad de Química; Uruguay.
Fil: Larcher, Roberto. Universidad de la República. Facultad de Química; Uruguay.
Fil: Nicolini, Giorgio. Universidad de la República. Facultad de Química; Uruguay.
Fil: Dellacassa, Eduardo Santiago. Fondazione Edmund Mach di San Michele all'Adige. Centro Trasferimento Tecnologico; Italia.
Ethnopharmacological relevance: ophidian accidents are a serious public health problem in Argentina; the Bothrops species is responsible for 97% of these accidents, and in particular, B. diporus is responsible for 80% of them. In the northeast of the country (Corrientes Provinces), Cissampelos pareira L. (Menispermaceae) is commonly used against the venom of B. diporus; its use is described in almost all ethnobotanical literature from countries where the plant grows. Aim of the study: in this study, the in vitro and in vivo antivenom activities of C. pareira extracts were evaluated against B. diporus venom, with a particular focus on the local effects associated with envenoming. The seasonal influence on the chemical composition of the active extracts was also studied, in order determine the associated range of variability and its influence on the antivenom activity. Materials and Methods: this research was conducted using aerial parts (leaves, flowers, tender stems) and roots of Cissampelos pareira collected from two different phytogeographic regions of Corrientes (Argentina); Paso de la Patria and Lomas de Vallejos. In addition, to perform a seasonal analysis and to evaluate the metabolic stability, material was collected at three different growth stages. In vivo and in vitro anti-snake venom activities were tested, and a bio-guided chromatographic separation was performed in order to determine the active chemicals involved. The fractions obtained were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and the chemical profile of the most active constituent was analyzed by ultra high-performance liquid chromatography coupled to quadrupole/high-resolution mass spectrometry (Q-Orbitrap). (UHPLC-MS). Results: the alcoholic extract was found to be the most active The bio-guided fractionation allowed selection one fraction to be analyzed by UHPLC-MS in order to identify the components responsible for the activities found; this identified five possible flavonoids. Conclusions: our studies of the activity of C. pareira against the venom of B. diporus have confirmed that this species possesses inhibitory effects in both in vitro and in vivo models. Moreover, the present data demonstrate that certain flavonoids may mitigate some of the venom-induced local tissue damage.
description Fil: Ricciardi Verrastro, Bárbara Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
publishDate 2018
dc.date.none.fl_str_mv 2018-02-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Ricciardi Verrastro, Bárbara Verónica, et al., 2018. The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom. Journal of Ethnopharmacology. Ámsterdam: Elsevier, vol. 212, p. 36-42. ISSN 0378-8741.
0378-8741
http://repositorio.unne.edu.ar/handle/123456789/30782
identifier_str_mv Ricciardi Verrastro, Bárbara Verónica, et al., 2018. The effects of Cissampelos pareira extract on envenomation induced by Bothropsdiporus snake venom. Journal of Ethnopharmacology. Ámsterdam: Elsevier, vol. 212, p. 36-42. ISSN 0378-8741.
0378-8741
url http://repositorio.unne.edu.ar/handle/123456789/30782
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.jep.2017.09.015
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Ethnopharmacology, 2018, vol. 212, p. 36-42.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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