Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae)

Autores
Oliveira, Vanessa Costa de; Lanari, Laura Cecilia; Hajos, Silvia Elvira; de Roodt, Adolfo Rafael
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report a study of toxic and enzymatic activities of Bothrops neuwiedi complex venoms collected from specimens of different regions of Argentina and a pool of these same venoms. Were determined lethal, hemorrhagic and pro-coagulant (plasma and fibrinogen) doses and the neutralization of these activities by a bivalent antivenom. The electrophoretic pattern of different regions venom was studied by SDS–PAGE. All samples exhibited lethal potencies, hemorrhagic and coagulant (plasma and fibrinogen) activities with potencies concordant with previous studies. The only conspicuous difference in the toxicological pattern of Bothrops diporus venoms was the low-thrombin-like activity found in one sample. The antivenom used in this study could neutralize all the toxic activities tested and the neutralizing potency of the antivenom was comparable for all samples. Despite the wide distribution of B. neuwiedi complex throughout Argentina and the evident morphological variation between B. diporus (B. neuwiedi complex), this study establishes a remarkably similar toxicity profile throughout its range. This is the first systematic study on the regional variation of enzymatic and toxic activities of venom from species belonging to the B. neuwiedi complex, one of the snakes of highest sanitary importance in South America and their neutralization by the type of antivenom most commonly used in the South of South America.
Fil: Oliveira, Vanessa Costa de . Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patologia. Centro de Patologia Experimental; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Inmunología; Argentina
Fil: Lanari, Laura Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Hajos, Silvia Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Inmunología; Argentina
Fil: de Roodt, Adolfo Rafael. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patologia. Centro de Patologia Experimental; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Materia
Bothrops Neuwiedi Complex
Venoms
Antivenom
Toxicity
Bothrops Diporus
Variability
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13912
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13912
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae)Oliveira, Vanessa Costa de Lanari, Laura CeciliaHajos, Silvia Elvirade Roodt, Adolfo RafaelBothrops Neuwiedi ComplexVenomsAntivenomToxicityBothrops DiporusVariabilityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We report a study of toxic and enzymatic activities of Bothrops neuwiedi complex venoms collected from specimens of different regions of Argentina and a pool of these same venoms. Were determined lethal, hemorrhagic and pro-coagulant (plasma and fibrinogen) doses and the neutralization of these activities by a bivalent antivenom. The electrophoretic pattern of different regions venom was studied by SDS–PAGE. All samples exhibited lethal potencies, hemorrhagic and coagulant (plasma and fibrinogen) activities with potencies concordant with previous studies. The only conspicuous difference in the toxicological pattern of Bothrops diporus venoms was the low-thrombin-like activity found in one sample. The antivenom used in this study could neutralize all the toxic activities tested and the neutralizing potency of the antivenom was comparable for all samples. Despite the wide distribution of B. neuwiedi complex throughout Argentina and the evident morphological variation between B. diporus (B. neuwiedi complex), this study establishes a remarkably similar toxicity profile throughout its range. This is the first systematic study on the regional variation of enzymatic and toxic activities of venom from species belonging to the B. neuwiedi complex, one of the snakes of highest sanitary importance in South America and their neutralization by the type of antivenom most commonly used in the South of South America.Fil: Oliveira, Vanessa Costa de . Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patologia. Centro de Patologia Experimental; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Inmunología; ArgentinaFil: Lanari, Laura Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; ArgentinaFil: Hajos, Silvia Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Inmunología; ArgentinaFil: de Roodt, Adolfo Rafael. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patologia. Centro de Patologia Experimental; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; ArgentinaElsevier2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13912Oliveira, Vanessa Costa de ; Lanari, Laura Cecilia; Hajos, Silvia Elvira; de Roodt, Adolfo Rafael; Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae); Elsevier; Toxicon; 57; 5; 4-2011; 680-6850041-0101enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0041010111000298info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2011.01.012info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:20:47Zoai:ri.conicet.gov.ar:11336/13912instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:20:47.799CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae)
title Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae)
spellingShingle Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae)
Oliveira, Vanessa Costa de
Bothrops Neuwiedi Complex
Venoms
Antivenom
Toxicity
Bothrops Diporus
Variability
title_short Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae)
title_full Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae)
title_fullStr Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae)
title_full_unstemmed Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae)
title_sort Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae)
dc.creator.none.fl_str_mv Oliveira, Vanessa Costa de
Lanari, Laura Cecilia
Hajos, Silvia Elvira
de Roodt, Adolfo Rafael
author Oliveira, Vanessa Costa de
author_facet Oliveira, Vanessa Costa de
Lanari, Laura Cecilia
Hajos, Silvia Elvira
de Roodt, Adolfo Rafael
author_role author
author2 Lanari, Laura Cecilia
Hajos, Silvia Elvira
de Roodt, Adolfo Rafael
author2_role author
author
author
dc.subject.none.fl_str_mv Bothrops Neuwiedi Complex
Venoms
Antivenom
Toxicity
Bothrops Diporus
Variability
topic Bothrops Neuwiedi Complex
Venoms
Antivenom
Toxicity
Bothrops Diporus
Variability
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report a study of toxic and enzymatic activities of Bothrops neuwiedi complex venoms collected from specimens of different regions of Argentina and a pool of these same venoms. Were determined lethal, hemorrhagic and pro-coagulant (plasma and fibrinogen) doses and the neutralization of these activities by a bivalent antivenom. The electrophoretic pattern of different regions venom was studied by SDS–PAGE. All samples exhibited lethal potencies, hemorrhagic and coagulant (plasma and fibrinogen) activities with potencies concordant with previous studies. The only conspicuous difference in the toxicological pattern of Bothrops diporus venoms was the low-thrombin-like activity found in one sample. The antivenom used in this study could neutralize all the toxic activities tested and the neutralizing potency of the antivenom was comparable for all samples. Despite the wide distribution of B. neuwiedi complex throughout Argentina and the evident morphological variation between B. diporus (B. neuwiedi complex), this study establishes a remarkably similar toxicity profile throughout its range. This is the first systematic study on the regional variation of enzymatic and toxic activities of venom from species belonging to the B. neuwiedi complex, one of the snakes of highest sanitary importance in South America and their neutralization by the type of antivenom most commonly used in the South of South America.
Fil: Oliveira, Vanessa Costa de . Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patologia. Centro de Patologia Experimental; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Inmunología; Argentina
Fil: Lanari, Laura Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
Fil: Hajos, Silvia Elvira. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Inmunología; Argentina
Fil: de Roodt, Adolfo Rafael. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patologia. Centro de Patologia Experimental; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina
description We report a study of toxic and enzymatic activities of Bothrops neuwiedi complex venoms collected from specimens of different regions of Argentina and a pool of these same venoms. Were determined lethal, hemorrhagic and pro-coagulant (plasma and fibrinogen) doses and the neutralization of these activities by a bivalent antivenom. The electrophoretic pattern of different regions venom was studied by SDS–PAGE. All samples exhibited lethal potencies, hemorrhagic and coagulant (plasma and fibrinogen) activities with potencies concordant with previous studies. The only conspicuous difference in the toxicological pattern of Bothrops diporus venoms was the low-thrombin-like activity found in one sample. The antivenom used in this study could neutralize all the toxic activities tested and the neutralizing potency of the antivenom was comparable for all samples. Despite the wide distribution of B. neuwiedi complex throughout Argentina and the evident morphological variation between B. diporus (B. neuwiedi complex), this study establishes a remarkably similar toxicity profile throughout its range. This is the first systematic study on the regional variation of enzymatic and toxic activities of venom from species belonging to the B. neuwiedi complex, one of the snakes of highest sanitary importance in South America and their neutralization by the type of antivenom most commonly used in the South of South America.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13912
Oliveira, Vanessa Costa de ; Lanari, Laura Cecilia; Hajos, Silvia Elvira; de Roodt, Adolfo Rafael; Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae); Elsevier; Toxicon; 57; 5; 4-2011; 680-685
0041-0101
url http://hdl.handle.net/11336/13912
identifier_str_mv Oliveira, Vanessa Costa de ; Lanari, Laura Cecilia; Hajos, Silvia Elvira; de Roodt, Adolfo Rafael; Toxicity of Bothrops neuwiedi complex (yarará chica) venom from different regions of Argentina (Serpentes, Viperidae); Elsevier; Toxicon; 57; 5; 4-2011; 680-685
0041-0101
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0041010111000298
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2011.01.012
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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