Genetic response of Paspalum plicatulum to genome duplication

Autores
Weihmüller, Emilse; Beltrán, Celina; Sartor, María Esperanza; Espinoza, Francisco; Spampinato, Claudia; Pessino, Silvina Claudia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Weihmüller, Emilse. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Beltrán, Celina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Argentina.
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Spampinato, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Paspalum plicatulum is a perennial rhizomatous grass with natural diploid and polyploid cytotypes. In this study, we investigated the occurrence of sequence polymorphisms arising immediately after genome autoduplication in this species. Two mixoploid plants (4C and 7D) were previously obtained through colchicine treatment of seeds generated by open pollination of a diploid plant (H14-2x). Diploid and tetraploid sectors from both mixoploids were dissected to generate two ploidy series (4C- 2x/4C-4x and 7D-2x/7D-4x). Molecular fingerprints were generated from the maternal plant H14-2x, both ploidy series (4C-2x/4C-4x and 7D-2x/7D-4x), and a tetraploid plant (C1) produced by selfing 7D-4x. Our results indicate that immediately after polyploidization P. plicatulum suffers genetic rearrangements affecting *28–38 % of the genome. Band gain and loss were equally prevalent at a statistically significant level. At least 5.62 % of the genome experimented recurrent genetic variation in a non-random basis with a confidence of 94.88 %. A significant proportion of novel bands (36 out of 195; 18.4 %) was detected in the C1 tetraploid plant. Half of these bands were not amplified in either H14-2x or 7D-4x, while the remainders were present in H14-2x but absent in 7D-4x. Our results indicate the occurrence of a considerable number of genetic changes in P. plicatulum immediately after polyploidization, some of which were recurrently detected in different independent events. Moreover, we confirmed that after polyploidization, lost ancestral alleles were spontaneously recovered in further generations, a phenomenon previously reported by other research groups.
Fuente
Genética, vol. 142, no. 3, p. 227- 234.
Materia
Autopolyploidy
Genetic response
Genomic shock
Molecular markers
Paspalum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/28624

id RIUNNE_396ae5bb83f90407e46a8d834692082a
oai_identifier_str oai:repositorio.unne.edu.ar:123456789/28624
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Genetic response of Paspalum plicatulum to genome duplicationWeihmüller, EmilseBeltrán, CelinaSartor, María EsperanzaEspinoza, FranciscoSpampinato, ClaudiaPessino, Silvina ClaudiaAutopolyploidyGenetic responseGenomic shockMolecular markersPaspalumFil: Weihmüller, Emilse. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Beltrán, Celina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Argentina.Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Spampinato, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; ArgentinaPaspalum plicatulum is a perennial rhizomatous grass with natural diploid and polyploid cytotypes. In this study, we investigated the occurrence of sequence polymorphisms arising immediately after genome autoduplication in this species. Two mixoploid plants (4C and 7D) were previously obtained through colchicine treatment of seeds generated by open pollination of a diploid plant (H14-2x). Diploid and tetraploid sectors from both mixoploids were dissected to generate two ploidy series (4C- 2x/4C-4x and 7D-2x/7D-4x). Molecular fingerprints were generated from the maternal plant H14-2x, both ploidy series (4C-2x/4C-4x and 7D-2x/7D-4x), and a tetraploid plant (C1) produced by selfing 7D-4x. Our results indicate that immediately after polyploidization P. plicatulum suffers genetic rearrangements affecting *28–38 % of the genome. Band gain and loss were equally prevalent at a statistically significant level. At least 5.62 % of the genome experimented recurrent genetic variation in a non-random basis with a confidence of 94.88 %. A significant proportion of novel bands (36 out of 195; 18.4 %) was detected in the C1 tetraploid plant. Half of these bands were not amplified in either H14-2x or 7D-4x, while the remainders were present in H14-2x but absent in 7D-4x. Our results indicate the occurrence of a considerable number of genetic changes in P. plicatulum immediately after polyploidization, some of which were recurrently detected in different independent events. Moreover, we confirmed that after polyploidization, lost ancestral alleles were spontaneously recovered in further generations, a phenomenon previously reported by other research groups.Springer International Publishing2014-05-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 227-234application/pdfWeihmüller, Emilse, et al., 2014. Genetic response of Paspalum plicatulum to genome duplication. Genética. New York: Springer International Publishing, vol. 142, no. 3, p. 227- 234. ISSN 0016-6707.0016-6707http://repositorio.unne.edu.ar/handle/123456789/28624Genética, vol. 142, no. 3, p. 227- 234.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttp://dx.doi.org/10.1007/s10709-014-9769-2info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:30:11Zoai:repositorio.unne.edu.ar:123456789/28624instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:11.442Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Genetic response of Paspalum plicatulum to genome duplication
title Genetic response of Paspalum plicatulum to genome duplication
spellingShingle Genetic response of Paspalum plicatulum to genome duplication
Weihmüller, Emilse
Autopolyploidy
Genetic response
Genomic shock
Molecular markers
Paspalum
title_short Genetic response of Paspalum plicatulum to genome duplication
title_full Genetic response of Paspalum plicatulum to genome duplication
title_fullStr Genetic response of Paspalum plicatulum to genome duplication
title_full_unstemmed Genetic response of Paspalum plicatulum to genome duplication
title_sort Genetic response of Paspalum plicatulum to genome duplication
dc.creator.none.fl_str_mv Weihmüller, Emilse
Beltrán, Celina
Sartor, María Esperanza
Espinoza, Francisco
Spampinato, Claudia
Pessino, Silvina Claudia
author Weihmüller, Emilse
author_facet Weihmüller, Emilse
Beltrán, Celina
Sartor, María Esperanza
Espinoza, Francisco
Spampinato, Claudia
Pessino, Silvina Claudia
author_role author
author2 Beltrán, Celina
Sartor, María Esperanza
Espinoza, Francisco
Spampinato, Claudia
Pessino, Silvina Claudia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Autopolyploidy
Genetic response
Genomic shock
Molecular markers
Paspalum
topic Autopolyploidy
Genetic response
Genomic shock
Molecular markers
Paspalum
dc.description.none.fl_txt_mv Fil: Weihmüller, Emilse. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Beltrán, Celina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Argentina.
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Spampinato, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Paspalum plicatulum is a perennial rhizomatous grass with natural diploid and polyploid cytotypes. In this study, we investigated the occurrence of sequence polymorphisms arising immediately after genome autoduplication in this species. Two mixoploid plants (4C and 7D) were previously obtained through colchicine treatment of seeds generated by open pollination of a diploid plant (H14-2x). Diploid and tetraploid sectors from both mixoploids were dissected to generate two ploidy series (4C- 2x/4C-4x and 7D-2x/7D-4x). Molecular fingerprints were generated from the maternal plant H14-2x, both ploidy series (4C-2x/4C-4x and 7D-2x/7D-4x), and a tetraploid plant (C1) produced by selfing 7D-4x. Our results indicate that immediately after polyploidization P. plicatulum suffers genetic rearrangements affecting *28–38 % of the genome. Band gain and loss were equally prevalent at a statistically significant level. At least 5.62 % of the genome experimented recurrent genetic variation in a non-random basis with a confidence of 94.88 %. A significant proportion of novel bands (36 out of 195; 18.4 %) was detected in the C1 tetraploid plant. Half of these bands were not amplified in either H14-2x or 7D-4x, while the remainders were present in H14-2x but absent in 7D-4x. Our results indicate the occurrence of a considerable number of genetic changes in P. plicatulum immediately after polyploidization, some of which were recurrently detected in different independent events. Moreover, we confirmed that after polyploidization, lost ancestral alleles were spontaneously recovered in further generations, a phenomenon previously reported by other research groups.
description Fil: Weihmüller, Emilse. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
publishDate 2014
dc.date.none.fl_str_mv 2014-05-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Weihmüller, Emilse, et al., 2014. Genetic response of Paspalum plicatulum to genome duplication. Genética. New York: Springer International Publishing, vol. 142, no. 3, p. 227- 234. ISSN 0016-6707.
0016-6707
http://repositorio.unne.edu.ar/handle/123456789/28624
identifier_str_mv Weihmüller, Emilse, et al., 2014. Genetic response of Paspalum plicatulum to genome duplication. Genética. New York: Springer International Publishing, vol. 142, no. 3, p. 227- 234. ISSN 0016-6707.
0016-6707
url http://repositorio.unne.edu.ar/handle/123456789/28624
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://dx.doi.org/10.1007/s10709-014-9769-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 227-234
application/pdf
dc.publisher.none.fl_str_mv Springer International Publishing
publisher.none.fl_str_mv Springer International Publishing
dc.source.none.fl_str_mv Genética, vol. 142, no. 3, p. 227- 234.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1844621683929907200
score 12.559606