Genetic response of Paspalum plicatulum to genome duplication
- Autores
- Weihmüller, Emilse; Beltrán, Celina; Sartor, María Esperanza; Espinoza, Francisco; Spampinato, Claudia; Pessino, Silvina Claudia
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Weihmüller, Emilse. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Beltrán, Celina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.
Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Argentina.
Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Spampinato, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
Paspalum plicatulum is a perennial rhizomatous grass with natural diploid and polyploid cytotypes. In this study, we investigated the occurrence of sequence polymorphisms arising immediately after genome autoduplication in this species. Two mixoploid plants (4C and 7D) were previously obtained through colchicine treatment of seeds generated by open pollination of a diploid plant (H14-2x). Diploid and tetraploid sectors from both mixoploids were dissected to generate two ploidy series (4C- 2x/4C-4x and 7D-2x/7D-4x). Molecular fingerprints were generated from the maternal plant H14-2x, both ploidy series (4C-2x/4C-4x and 7D-2x/7D-4x), and a tetraploid plant (C1) produced by selfing 7D-4x. Our results indicate that immediately after polyploidization P. plicatulum suffers genetic rearrangements affecting *28–38 % of the genome. Band gain and loss were equally prevalent at a statistically significant level. At least 5.62 % of the genome experimented recurrent genetic variation in a non-random basis with a confidence of 94.88 %. A significant proportion of novel bands (36 out of 195; 18.4 %) was detected in the C1 tetraploid plant. Half of these bands were not amplified in either H14-2x or 7D-4x, while the remainders were present in H14-2x but absent in 7D-4x. Our results indicate the occurrence of a considerable number of genetic changes in P. plicatulum immediately after polyploidization, some of which were recurrently detected in different independent events. Moreover, we confirmed that after polyploidization, lost ancestral alleles were spontaneously recovered in further generations, a phenomenon previously reported by other research groups. - Fuente
- Genética, vol. 142, no. 3, p. 227- 234.
- Materia
-
Autopolyploidy
Genetic response
Genomic shock
Molecular markers
Paspalum - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Universidad Nacional del Nordeste
- OAI Identificador
- oai:repositorio.unne.edu.ar:123456789/28624
Ver los metadatos del registro completo
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Genetic response of Paspalum plicatulum to genome duplicationWeihmüller, EmilseBeltrán, CelinaSartor, María EsperanzaEspinoza, FranciscoSpampinato, ClaudiaPessino, Silvina ClaudiaAutopolyploidyGenetic responseGenomic shockMolecular markersPaspalumFil: Weihmüller, Emilse. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Beltrán, Celina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina.Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Argentina.Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Spampinato, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; ArgentinaPaspalum plicatulum is a perennial rhizomatous grass with natural diploid and polyploid cytotypes. In this study, we investigated the occurrence of sequence polymorphisms arising immediately after genome autoduplication in this species. Two mixoploid plants (4C and 7D) were previously obtained through colchicine treatment of seeds generated by open pollination of a diploid plant (H14-2x). Diploid and tetraploid sectors from both mixoploids were dissected to generate two ploidy series (4C- 2x/4C-4x and 7D-2x/7D-4x). Molecular fingerprints were generated from the maternal plant H14-2x, both ploidy series (4C-2x/4C-4x and 7D-2x/7D-4x), and a tetraploid plant (C1) produced by selfing 7D-4x. Our results indicate that immediately after polyploidization P. plicatulum suffers genetic rearrangements affecting *28–38 % of the genome. Band gain and loss were equally prevalent at a statistically significant level. At least 5.62 % of the genome experimented recurrent genetic variation in a non-random basis with a confidence of 94.88 %. A significant proportion of novel bands (36 out of 195; 18.4 %) was detected in the C1 tetraploid plant. Half of these bands were not amplified in either H14-2x or 7D-4x, while the remainders were present in H14-2x but absent in 7D-4x. Our results indicate the occurrence of a considerable number of genetic changes in P. plicatulum immediately after polyploidization, some of which were recurrently detected in different independent events. Moreover, we confirmed that after polyploidization, lost ancestral alleles were spontaneously recovered in further generations, a phenomenon previously reported by other research groups.Springer International Publishing2014-05-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 227-234application/pdfWeihmüller, Emilse, et al., 2014. Genetic response of Paspalum plicatulum to genome duplication. Genética. New York: Springer International Publishing, vol. 142, no. 3, p. 227- 234. ISSN 0016-6707.0016-6707http://repositorio.unne.edu.ar/handle/123456789/28624Genética, vol. 142, no. 3, p. 227- 234.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttp://dx.doi.org/10.1007/s10709-014-9769-2info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-09-29T14:30:11Zoai:repositorio.unne.edu.ar:123456789/28624instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-09-29 14:30:11.442Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse |
dc.title.none.fl_str_mv |
Genetic response of Paspalum plicatulum to genome duplication |
title |
Genetic response of Paspalum plicatulum to genome duplication |
spellingShingle |
Genetic response of Paspalum plicatulum to genome duplication Weihmüller, Emilse Autopolyploidy Genetic response Genomic shock Molecular markers Paspalum |
title_short |
Genetic response of Paspalum plicatulum to genome duplication |
title_full |
Genetic response of Paspalum plicatulum to genome duplication |
title_fullStr |
Genetic response of Paspalum plicatulum to genome duplication |
title_full_unstemmed |
Genetic response of Paspalum plicatulum to genome duplication |
title_sort |
Genetic response of Paspalum plicatulum to genome duplication |
dc.creator.none.fl_str_mv |
Weihmüller, Emilse Beltrán, Celina Sartor, María Esperanza Espinoza, Francisco Spampinato, Claudia Pessino, Silvina Claudia |
author |
Weihmüller, Emilse |
author_facet |
Weihmüller, Emilse Beltrán, Celina Sartor, María Esperanza Espinoza, Francisco Spampinato, Claudia Pessino, Silvina Claudia |
author_role |
author |
author2 |
Beltrán, Celina Sartor, María Esperanza Espinoza, Francisco Spampinato, Claudia Pessino, Silvina Claudia |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Autopolyploidy Genetic response Genomic shock Molecular markers Paspalum |
topic |
Autopolyploidy Genetic response Genomic shock Molecular markers Paspalum |
dc.description.none.fl_txt_mv |
Fil: Weihmüller, Emilse. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Fil: Beltrán, Celina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Fil: Sartor, María Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste. Argentina. Fil: Espinoza, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina. Fil: Spampinato, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina Paspalum plicatulum is a perennial rhizomatous grass with natural diploid and polyploid cytotypes. In this study, we investigated the occurrence of sequence polymorphisms arising immediately after genome autoduplication in this species. Two mixoploid plants (4C and 7D) were previously obtained through colchicine treatment of seeds generated by open pollination of a diploid plant (H14-2x). Diploid and tetraploid sectors from both mixoploids were dissected to generate two ploidy series (4C- 2x/4C-4x and 7D-2x/7D-4x). Molecular fingerprints were generated from the maternal plant H14-2x, both ploidy series (4C-2x/4C-4x and 7D-2x/7D-4x), and a tetraploid plant (C1) produced by selfing 7D-4x. Our results indicate that immediately after polyploidization P. plicatulum suffers genetic rearrangements affecting *28–38 % of the genome. Band gain and loss were equally prevalent at a statistically significant level. At least 5.62 % of the genome experimented recurrent genetic variation in a non-random basis with a confidence of 94.88 %. A significant proportion of novel bands (36 out of 195; 18.4 %) was detected in the C1 tetraploid plant. Half of these bands were not amplified in either H14-2x or 7D-4x, while the remainders were present in H14-2x but absent in 7D-4x. Our results indicate the occurrence of a considerable number of genetic changes in P. plicatulum immediately after polyploidization, some of which were recurrently detected in different independent events. Moreover, we confirmed that after polyploidization, lost ancestral alleles were spontaneously recovered in further generations, a phenomenon previously reported by other research groups. |
description |
Fil: Weihmüller, Emilse. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05-25 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
Weihmüller, Emilse, et al., 2014. Genetic response of Paspalum plicatulum to genome duplication. Genética. New York: Springer International Publishing, vol. 142, no. 3, p. 227- 234. ISSN 0016-6707. 0016-6707 http://repositorio.unne.edu.ar/handle/123456789/28624 |
identifier_str_mv |
Weihmüller, Emilse, et al., 2014. Genetic response of Paspalum plicatulum to genome duplication. Genética. New York: Springer International Publishing, vol. 142, no. 3, p. 227- 234. ISSN 0016-6707. 0016-6707 |
url |
http://repositorio.unne.edu.ar/handle/123456789/28624 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://dx.doi.org/10.1007/s10709-014-9769-2 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Atribución-NoComercial-SinDerivadas 2.5 Argentina |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ Atribución-NoComercial-SinDerivadas 2.5 Argentina |
dc.format.none.fl_str_mv |
application/pdf p. 227-234 application/pdf |
dc.publisher.none.fl_str_mv |
Springer International Publishing |
publisher.none.fl_str_mv |
Springer International Publishing |
dc.source.none.fl_str_mv |
Genética, vol. 142, no. 3, p. 227- 234. reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) instname:Universidad Nacional del Nordeste |
reponame_str |
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
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Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) |
instname_str |
Universidad Nacional del Nordeste |
repository.name.fl_str_mv |
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste |
repository.mail.fl_str_mv |
ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar |
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12.559606 |