Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)

Autores
Dolce, Natalia Raquel; Medina, Ricardo Daniel; Mroginski, Luis Amado; Rey, Hebe Yolanda
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Seeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed a pyrene. Embryos are undeveloped when fruits reach maturity. As a consequence, germination is low even after several months under proper conditions. In the present study, we present reliable and reproducible techniques that enhance seed germination of Ilex brasiliensis, I. pseudoboxus and I. theezans by aseptically sowing whole pyrenes. The highest germination percentages (53-92%) were reached by in vitro culturing whole pyrenes on solidified (0.65% agar) quarter-strength Murashige and Skoog medium with 3% sucrose and 0.1 mg l-1 zeatin, and incubating in a growth room at 27 ± 2°C with a 14-hour photoperiod. Thus, the in vitro culture of whole pyrenes constitutes an easy and efficient technique to obtain quick germination of these Ilex species and may be an alternative to embryo rescue. Rapid progress in the application of this method to breeding and preservation programmes of Ilex species is expected.
Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Medina, Ricardo Daniel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Rey, Hebe R. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Rey, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fuente
Seed Science and technology, vol. 43, no. 2, p.1-5.
Materia
Germination
Aquifoliaceae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/9156
Acceder
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network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)Dolce, Natalia RaquelMedina, Ricardo DanielMroginski, Luis AmadoRey, Hebe YolandaGerminationAquifoliaceaeSeeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed a pyrene. Embryos are undeveloped when fruits reach maturity. As a consequence, germination is low even after several months under proper conditions. In the present study, we present reliable and reproducible techniques that enhance seed germination of Ilex brasiliensis, I. pseudoboxus and I. theezans by aseptically sowing whole pyrenes. The highest germination percentages (53-92%) were reached by in vitro culturing whole pyrenes on solidified (0.65% agar) quarter-strength Murashige and Skoog medium with 3% sucrose and 0.1 mg l-1 zeatin, and incubating in a growth room at 27 ± 2°C with a 14-hour photoperiod. Thus, the in vitro culture of whole pyrenes constitutes an easy and efficient technique to obtain quick germination of these Ilex species and may be an alternative to embryo rescue. Rapid progress in the application of this method to breeding and preservation programmes of Ilex species is expected.Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Medina, Ricardo Daniel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Rey, Hebe R. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Rey, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Seed testing. Association International2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp.1-5application/pdfDolce, Natalia Raquel, et al, 2015. Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae). Seed Science and technology. Estados Unidos: International Seed Testing Association, vol. 43, no 2, p.1-5. ISSN 1819-5717.0251-0952http://repositorio.unne.edu.ar/handle/123456789/9156Seed Science and technology, vol. 43, no. 2, p.1-5.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://www.ingentaconnect.com/content/ista/sst/2015/00000043/00000002/art00013info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-10-16T10:07:56Zoai:repositorio.unne.edu.ar:123456789/9156instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-10-16 10:07:57.213Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
title Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
spellingShingle Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
Dolce, Natalia Raquel
Germination
Aquifoliaceae
title_short Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
title_full Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
title_fullStr Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
title_full_unstemmed Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
title_sort Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae)
dc.creator.none.fl_str_mv Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author Dolce, Natalia Raquel
author_facet Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author_role author
author2 Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, Hebe Yolanda
author2_role author
author
author
dc.subject.none.fl_str_mv Germination
Aquifoliaceae
topic Germination
Aquifoliaceae
dc.description.none.fl_txt_mv Seeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed a pyrene. Embryos are undeveloped when fruits reach maturity. As a consequence, germination is low even after several months under proper conditions. In the present study, we present reliable and reproducible techniques that enhance seed germination of Ilex brasiliensis, I. pseudoboxus and I. theezans by aseptically sowing whole pyrenes. The highest germination percentages (53-92%) were reached by in vitro culturing whole pyrenes on solidified (0.65% agar) quarter-strength Murashige and Skoog medium with 3% sucrose and 0.1 mg l-1 zeatin, and incubating in a growth room at 27 ± 2°C with a 14-hour photoperiod. Thus, the in vitro culture of whole pyrenes constitutes an easy and efficient technique to obtain quick germination of these Ilex species and may be an alternative to embryo rescue. Rapid progress in the application of this method to breeding and preservation programmes of Ilex species is expected.
Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Medina, Ricardo Daniel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
Fil: Rey, Hebe R. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Rey, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.
description Seeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed a pyrene. Embryos are undeveloped when fruits reach maturity. As a consequence, germination is low even after several months under proper conditions. In the present study, we present reliable and reproducible techniques that enhance seed germination of Ilex brasiliensis, I. pseudoboxus and I. theezans by aseptically sowing whole pyrenes. The highest germination percentages (53-92%) were reached by in vitro culturing whole pyrenes on solidified (0.65% agar) quarter-strength Murashige and Skoog medium with 3% sucrose and 0.1 mg l-1 zeatin, and incubating in a growth room at 27 ± 2°C with a 14-hour photoperiod. Thus, the in vitro culture of whole pyrenes constitutes an easy and efficient technique to obtain quick germination of these Ilex species and may be an alternative to embryo rescue. Rapid progress in the application of this method to breeding and preservation programmes of Ilex species is expected.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Dolce, Natalia Raquel, et al, 2015. Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae). Seed Science and technology. Estados Unidos: International Seed Testing Association, vol. 43, no 2, p.1-5. ISSN 1819-5717.
0251-0952
http://repositorio.unne.edu.ar/handle/123456789/9156
identifier_str_mv Dolce, Natalia Raquel, et al, 2015. Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasi liensis, I. pseudoboxus and I. theezans (Aquifoliaceae). Seed Science and technology. Estados Unidos: International Seed Testing Association, vol. 43, no 2, p.1-5. ISSN 1819-5717.
0251-0952
url http://repositorio.unne.edu.ar/handle/123456789/9156
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.ingentaconnect.com/content/ista/sst/2015/00000043/00000002/art00013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p.1-5
application/pdf
dc.publisher.none.fl_str_mv Seed testing. Association International
publisher.none.fl_str_mv Seed testing. Association International
dc.source.none.fl_str_mv Seed Science and technology, vol. 43, no. 2, p.1-5.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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score 12.712165

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