Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae)

Autores
Dolce, Natalia Raquel; Medina, Ricardo Daniel; Mroginski, Luis Amado; Rey, H. Y.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The seeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed pyrene, and have undeveloped embryos when fruits reach maturity. As a consequence, the seed germination rate is low even after several months under the proper conditions. In the present study, we present reliable and reproducible techniques that enhance seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans by aseptically sowing whole pyrenes. The highest germination percentages (53-92%) were reached by in vitro culturing whole pyrenes on solidified (agar 0.65%) quarter-strength Murashige and Skoog medium with 3% sucrose and 0.1 mg l-1 zeatin, and incubating in a growth room at 27±2oC with a 14 hours light photoperiod. Thus, the in vitro culture of whole pyrenes constitutes an easy and efficient technique to obtain a quick germination of these Ilex species and may be an alternative to embryo rescue. Rapid progress in the application of this method to breeding and preservation programs of Ilex species is expected.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Rey, H. Y.. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Materia
Seed Dormancy
Embryo Rescue
In Vitro Culture
Holly
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25084
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae)Dolce, Natalia RaquelMedina, Ricardo DanielMroginski, Luis AmadoRey, H. Y.Seed DormancyEmbryo RescueIn Vitro CultureHollyhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The seeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed pyrene, and have undeveloped embryos when fruits reach maturity. As a consequence, the seed germination rate is low even after several months under the proper conditions. In the present study, we present reliable and reproducible techniques that enhance seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans by aseptically sowing whole pyrenes. The highest germination percentages (53-92%) were reached by in vitro culturing whole pyrenes on solidified (agar 0.65%) quarter-strength Murashige and Skoog medium with 3% sucrose and 0.1 mg l-1 zeatin, and incubating in a growth room at 27±2oC with a 14 hours light photoperiod. Thus, the in vitro culture of whole pyrenes constitutes an easy and efficient technique to obtain a quick germination of these Ilex species and may be an alternative to embryo rescue. Rapid progress in the application of this method to breeding and preservation programs of Ilex species is expected.Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; ArgentinaFil: Rey, H. Y.. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaInternational Seed Testing Association2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25084Dolce, Natalia Raquel; Medina, Ricardo Daniel; Mroginski, Luis Amado; Rey, H. Y.; Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae); International Seed Testing Association; Seed Science And Technology; 43; 2; 8-2015; 273-2770251-09521819-5717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.15258/sst.2015.43.2.04info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/ista/sst/2015/00000043/00000002/art00013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:04Zoai:ri.conicet.gov.ar:11336/25084instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:05.05CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae)
title Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae)
spellingShingle Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae)
Dolce, Natalia Raquel
Seed Dormancy
Embryo Rescue
In Vitro Culture
Holly
title_short Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae)
title_full Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae)
title_fullStr Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae)
title_full_unstemmed Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae)
title_sort Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae)
dc.creator.none.fl_str_mv Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, H. Y.
author Dolce, Natalia Raquel
author_facet Dolce, Natalia Raquel
Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, H. Y.
author_role author
author2 Medina, Ricardo Daniel
Mroginski, Luis Amado
Rey, H. Y.
author2_role author
author
author
dc.subject.none.fl_str_mv Seed Dormancy
Embryo Rescue
In Vitro Culture
Holly
topic Seed Dormancy
Embryo Rescue
In Vitro Culture
Holly
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The seeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed pyrene, and have undeveloped embryos when fruits reach maturity. As a consequence, the seed germination rate is low even after several months under the proper conditions. In the present study, we present reliable and reproducible techniques that enhance seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans by aseptically sowing whole pyrenes. The highest germination percentages (53-92%) were reached by in vitro culturing whole pyrenes on solidified (agar 0.65%) quarter-strength Murashige and Skoog medium with 3% sucrose and 0.1 mg l-1 zeatin, and incubating in a growth room at 27±2oC with a 14 hours light photoperiod. Thus, the in vitro culture of whole pyrenes constitutes an easy and efficient technique to obtain a quick germination of these Ilex species and may be an alternative to embryo rescue. Rapid progress in the application of this method to breeding and preservation programs of Ilex species is expected.
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Rey, H. Y.. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
description The seeds of Ilex species are individually enclosed by a woody endocarp, forming a dispersal unit termed pyrene, and have undeveloped embryos when fruits reach maturity. As a consequence, the seed germination rate is low even after several months under the proper conditions. In the present study, we present reliable and reproducible techniques that enhance seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans by aseptically sowing whole pyrenes. The highest germination percentages (53-92%) were reached by in vitro culturing whole pyrenes on solidified (agar 0.65%) quarter-strength Murashige and Skoog medium with 3% sucrose and 0.1 mg l-1 zeatin, and incubating in a growth room at 27±2oC with a 14 hours light photoperiod. Thus, the in vitro culture of whole pyrenes constitutes an easy and efficient technique to obtain a quick germination of these Ilex species and may be an alternative to embryo rescue. Rapid progress in the application of this method to breeding and preservation programs of Ilex species is expected.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25084
Dolce, Natalia Raquel; Medina, Ricardo Daniel; Mroginski, Luis Amado; Rey, H. Y.; Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae); International Seed Testing Association; Seed Science And Technology; 43; 2; 8-2015; 273-277
0251-0952
1819-5717
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25084
identifier_str_mv Dolce, Natalia Raquel; Medina, Ricardo Daniel; Mroginski, Luis Amado; Rey, H. Y.; Sowing pyrenes under aseptic conditions enhances seed germination of Ilex brasiliensis, I. pseudoboxus, and I. theezans (Aquifoliaceae); International Seed Testing Association; Seed Science And Technology; 43; 2; 8-2015; 273-277
0251-0952
1819-5717
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.15258/sst.2015.43.2.04
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/ista/sst/2015/00000043/00000002/art00013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Seed Testing Association
publisher.none.fl_str_mv International Seed Testing Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269941299937280
score 13.13397

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