Plant regeneration from ilex spp. (aquifoliaceae) in vitro

• Autores: Sansberro, Pedro Alfonso; Rey, Hebe Yolanda; Mroginski, Luis Amado; Krivenki, Mario A.
• Año de publicación: 2001
• Idioma: español castellano
• Tipo de recurso: artículo
• Fuente: Biocell, 2001, vol. 25 no. 2, p. 139-146.
• Materia: Plant regeneration; Germination; Embryo culture; Holly; Propagation
• Institución: Universidad Nacional del Nordeste

Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Rey, Hebe Yolanda. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.
Fil: Krivenki, Mario A. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Cerro Azul; Argentina.
In vitro plant regeneration from nodal segments (containing one axillary bud) of seven species of the genus Ilex (l argentina, I. brevicuspis, I. dumosa, I. microdonta, I. pseudoboxus, I. taubertianb and I theezans ) were readily achieved through three steps: 1) shoot regeneration by in vitro culture of nodal segments in MS médium at 1/4 strength, plus 3% sucrose and 0.65% agar (1/4MS) and 0.5 |jM BA (45 days of culture); 2) Induction of rooting from regenerated shoots with 1/4MS (solidified with 2.5 g.L'1 “Phytagel”) with 7.3 pM IBA (7 days) and, 3) subculture of shoot on a fresh médium (1/4MS lacking plant growth regulators) during 21 days. Shoot regeneration of other three species ( I aquifolium, /. bmsiliensis and /. inlegerrima) were also obtained by in vitro culture of nodal segments. Shoot regeneration of/. aquifolium, I. brasiliensis, I. integerrima, I. microdonta, I. pseudoboxus, and I. taubertiana were also obtained by culture shoot tips on 1/4MS and 0.5 ]iM BA. Shoot regeneration from meristems o f l argentina, I. brevicuspis, I. dumosa, and I. theezans were readily achieved by in vitro culture on the same médium.