TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo
- Autores
- Belkacemi, Thabet; Niermann, Alexander; Hofmann, Laura; Wissenbach, Ulrich; Birnbaumer, Lutz; Leidinger, Petra; Backes, Christina; Meese, Eckart; Keller, Andreas; Bai, Xianshu; Scheller, Anja; Kirchhoff, Frank; Philipp, Stephan E.; Weissgerber, Petra; Flockerzi, Veit; Beck, Andreas
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Following brain injury astrocytes change into a reactive state, proliferate and grow into the site of lesion, a process called astrogliosis, initiated and regulated by changes in cytoplasmic Ca2+. Transient receptor potential canonical (TRPC) channels may contribute to Ca2+ influx but their presence and possible function in astrocytes is not known. By RT-PCR and RNA sequencing we identified transcripts of Trpc1, Trpc2, Trpc3, and Trpc4 in FACS-sorted glutamate aspartate transporter (GLAST)-positive cultured mouse cortical astrocytes and subcloned full-length Trpc1 and Trpc3 cDNAs from these cells. Ca2+ entry in cortical astrocytes depended on TRPC3 and was increased in the absence of Trpc1. After co-expression of Trpc1 and Trpc3 in HEK-293 cells both proteins co-immunoprecipitate and form functional heteromeric channels, with TRPC1 reducing TRPC3 activity. In vitro, lack of Trpc3 reduced astrocyte proliferation and migration whereas the TRPC3 gain-of-function moonwalker mutation and Trpc1 deficiency increased astrocyte migration. In vivo, astrogliosis and cortex edema following stab wound injury were reduced in Trpc3-/- but increased in Trpc1-/- mice. In summary, our results show a decisive contribution of TRPC3 to astrocyte Ca2+ signaling, which is even augmented in the absence of Trpc1, in particular following brain injury. Targeted therapies to reduce TRPC3 channel activity in astrocytes might therefore be beneficial in traumatic brain injury.
Fil: Belkacemi, Thabet. Universitat Saarland; Alemania
Fil: Niermann, Alexander. Universitat Saarland; Alemania
Fil: Hofmann, Laura. Universitat Saarland; Alemania
Fil: Wissenbach, Ulrich. Universitat Saarland; Alemania
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina ; Argentina. National Institute of Environmental Health Sciences; Estados Unidos
Fil: Leidinger, Petra. Universitat Saarland; Alemania
Fil: Backes, Christina. Universitat Saarland; Alemania
Fil: Meese, Eckart. Universitat Saarland; Alemania
Fil: Keller, Andreas. Universitat Saarland; Alemania
Fil: Bai, Xianshu. Universitat Saarland; Alemania
Fil: Scheller, Anja. Universitat Saarland; Alemania
Fil: Kirchhoff, Frank. Universitat Saarland; Alemania
Fil: Philipp, Stephan E.. Universitat Saarland; Alemania
Fil: Weissgerber, Petra. Universitat Saarland; Alemania
Fil: Flockerzi, Veit. Universitat Saarland; Alemania
Fil: Beck, Andreas. Universitat Saarland; Alemania - Materia
-
Glia
Ion Channels
Membrane Currents
Migration
Proliferation
Stab Wound Injury - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/49949
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oai:ri.conicet.gov.ar:11336/49949 |
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network_name_str |
CONICET Digital (CONICET) |
spelling |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivoBelkacemi, ThabetNiermann, AlexanderHofmann, LauraWissenbach, UlrichBirnbaumer, LutzLeidinger, PetraBackes, ChristinaMeese, EckartKeller, AndreasBai, XianshuScheller, AnjaKirchhoff, FrankPhilipp, Stephan E.Weissgerber, PetraFlockerzi, VeitBeck, AndreasGliaIon ChannelsMembrane CurrentsMigrationProliferationStab Wound Injuryhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Following brain injury astrocytes change into a reactive state, proliferate and grow into the site of lesion, a process called astrogliosis, initiated and regulated by changes in cytoplasmic Ca2+. Transient receptor potential canonical (TRPC) channels may contribute to Ca2+ influx but their presence and possible function in astrocytes is not known. By RT-PCR and RNA sequencing we identified transcripts of Trpc1, Trpc2, Trpc3, and Trpc4 in FACS-sorted glutamate aspartate transporter (GLAST)-positive cultured mouse cortical astrocytes and subcloned full-length Trpc1 and Trpc3 cDNAs from these cells. Ca2+ entry in cortical astrocytes depended on TRPC3 and was increased in the absence of Trpc1. After co-expression of Trpc1 and Trpc3 in HEK-293 cells both proteins co-immunoprecipitate and form functional heteromeric channels, with TRPC1 reducing TRPC3 activity. In vitro, lack of Trpc3 reduced astrocyte proliferation and migration whereas the TRPC3 gain-of-function moonwalker mutation and Trpc1 deficiency increased astrocyte migration. In vivo, astrogliosis and cortex edema following stab wound injury were reduced in Trpc3-/- but increased in Trpc1-/- mice. In summary, our results show a decisive contribution of TRPC3 to astrocyte Ca2+ signaling, which is even augmented in the absence of Trpc1, in particular following brain injury. Targeted therapies to reduce TRPC3 channel activity in astrocytes might therefore be beneficial in traumatic brain injury.Fil: Belkacemi, Thabet. Universitat Saarland; AlemaniaFil: Niermann, Alexander. Universitat Saarland; AlemaniaFil: Hofmann, Laura. Universitat Saarland; AlemaniaFil: Wissenbach, Ulrich. Universitat Saarland; AlemaniaFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina ; Argentina. National Institute of Environmental Health Sciences; Estados UnidosFil: Leidinger, Petra. Universitat Saarland; AlemaniaFil: Backes, Christina. Universitat Saarland; AlemaniaFil: Meese, Eckart. Universitat Saarland; AlemaniaFil: Keller, Andreas. Universitat Saarland; AlemaniaFil: Bai, Xianshu. Universitat Saarland; AlemaniaFil: Scheller, Anja. Universitat Saarland; AlemaniaFil: Kirchhoff, Frank. Universitat Saarland; AlemaniaFil: Philipp, Stephan E.. Universitat Saarland; AlemaniaFil: Weissgerber, Petra. Universitat Saarland; AlemaniaFil: Flockerzi, Veit. Universitat Saarland; AlemaniaFil: Beck, Andreas. Universitat Saarland; AlemaniaWiley-liss, Div John Wiley & Sons Inc2017-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49949Belkacemi, Thabet; Niermann, Alexander; Hofmann, Laura; Wissenbach, Ulrich; Birnbaumer, Lutz; et al.; TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo; Wiley-liss, Div John Wiley & Sons Inc; Glia; 65; 9; 6-2017; 1535-15490894-14911098-1136CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/glia.23180info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.23180info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:26Zoai:ri.conicet.gov.ar:11336/49949instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:26.911CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
title |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
spellingShingle |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo Belkacemi, Thabet Glia Ion Channels Membrane Currents Migration Proliferation Stab Wound Injury |
title_short |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
title_full |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
title_fullStr |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
title_full_unstemmed |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
title_sort |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
dc.creator.none.fl_str_mv |
Belkacemi, Thabet Niermann, Alexander Hofmann, Laura Wissenbach, Ulrich Birnbaumer, Lutz Leidinger, Petra Backes, Christina Meese, Eckart Keller, Andreas Bai, Xianshu Scheller, Anja Kirchhoff, Frank Philipp, Stephan E. Weissgerber, Petra Flockerzi, Veit Beck, Andreas |
author |
Belkacemi, Thabet |
author_facet |
Belkacemi, Thabet Niermann, Alexander Hofmann, Laura Wissenbach, Ulrich Birnbaumer, Lutz Leidinger, Petra Backes, Christina Meese, Eckart Keller, Andreas Bai, Xianshu Scheller, Anja Kirchhoff, Frank Philipp, Stephan E. Weissgerber, Petra Flockerzi, Veit Beck, Andreas |
author_role |
author |
author2 |
Niermann, Alexander Hofmann, Laura Wissenbach, Ulrich Birnbaumer, Lutz Leidinger, Petra Backes, Christina Meese, Eckart Keller, Andreas Bai, Xianshu Scheller, Anja Kirchhoff, Frank Philipp, Stephan E. Weissgerber, Petra Flockerzi, Veit Beck, Andreas |
author2_role |
author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Glia Ion Channels Membrane Currents Migration Proliferation Stab Wound Injury |
topic |
Glia Ion Channels Membrane Currents Migration Proliferation Stab Wound Injury |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Following brain injury astrocytes change into a reactive state, proliferate and grow into the site of lesion, a process called astrogliosis, initiated and regulated by changes in cytoplasmic Ca2+. Transient receptor potential canonical (TRPC) channels may contribute to Ca2+ influx but their presence and possible function in astrocytes is not known. By RT-PCR and RNA sequencing we identified transcripts of Trpc1, Trpc2, Trpc3, and Trpc4 in FACS-sorted glutamate aspartate transporter (GLAST)-positive cultured mouse cortical astrocytes and subcloned full-length Trpc1 and Trpc3 cDNAs from these cells. Ca2+ entry in cortical astrocytes depended on TRPC3 and was increased in the absence of Trpc1. After co-expression of Trpc1 and Trpc3 in HEK-293 cells both proteins co-immunoprecipitate and form functional heteromeric channels, with TRPC1 reducing TRPC3 activity. In vitro, lack of Trpc3 reduced astrocyte proliferation and migration whereas the TRPC3 gain-of-function moonwalker mutation and Trpc1 deficiency increased astrocyte migration. In vivo, astrogliosis and cortex edema following stab wound injury were reduced in Trpc3-/- but increased in Trpc1-/- mice. In summary, our results show a decisive contribution of TRPC3 to astrocyte Ca2+ signaling, which is even augmented in the absence of Trpc1, in particular following brain injury. Targeted therapies to reduce TRPC3 channel activity in astrocytes might therefore be beneficial in traumatic brain injury. Fil: Belkacemi, Thabet. Universitat Saarland; Alemania Fil: Niermann, Alexander. Universitat Saarland; Alemania Fil: Hofmann, Laura. Universitat Saarland; Alemania Fil: Wissenbach, Ulrich. Universitat Saarland; Alemania Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina ; Argentina. National Institute of Environmental Health Sciences; Estados Unidos Fil: Leidinger, Petra. Universitat Saarland; Alemania Fil: Backes, Christina. Universitat Saarland; Alemania Fil: Meese, Eckart. Universitat Saarland; Alemania Fil: Keller, Andreas. Universitat Saarland; Alemania Fil: Bai, Xianshu. Universitat Saarland; Alemania Fil: Scheller, Anja. Universitat Saarland; Alemania Fil: Kirchhoff, Frank. Universitat Saarland; Alemania Fil: Philipp, Stephan E.. Universitat Saarland; Alemania Fil: Weissgerber, Petra. Universitat Saarland; Alemania Fil: Flockerzi, Veit. Universitat Saarland; Alemania Fil: Beck, Andreas. Universitat Saarland; Alemania |
description |
Following brain injury astrocytes change into a reactive state, proliferate and grow into the site of lesion, a process called astrogliosis, initiated and regulated by changes in cytoplasmic Ca2+. Transient receptor potential canonical (TRPC) channels may contribute to Ca2+ influx but their presence and possible function in astrocytes is not known. By RT-PCR and RNA sequencing we identified transcripts of Trpc1, Trpc2, Trpc3, and Trpc4 in FACS-sorted glutamate aspartate transporter (GLAST)-positive cultured mouse cortical astrocytes and subcloned full-length Trpc1 and Trpc3 cDNAs from these cells. Ca2+ entry in cortical astrocytes depended on TRPC3 and was increased in the absence of Trpc1. After co-expression of Trpc1 and Trpc3 in HEK-293 cells both proteins co-immunoprecipitate and form functional heteromeric channels, with TRPC1 reducing TRPC3 activity. In vitro, lack of Trpc3 reduced astrocyte proliferation and migration whereas the TRPC3 gain-of-function moonwalker mutation and Trpc1 deficiency increased astrocyte migration. In vivo, astrogliosis and cortex edema following stab wound injury were reduced in Trpc3-/- but increased in Trpc1-/- mice. In summary, our results show a decisive contribution of TRPC3 to astrocyte Ca2+ signaling, which is even augmented in the absence of Trpc1, in particular following brain injury. Targeted therapies to reduce TRPC3 channel activity in astrocytes might therefore be beneficial in traumatic brain injury. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/49949 Belkacemi, Thabet; Niermann, Alexander; Hofmann, Laura; Wissenbach, Ulrich; Birnbaumer, Lutz; et al.; TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo; Wiley-liss, Div John Wiley & Sons Inc; Glia; 65; 9; 6-2017; 1535-1549 0894-1491 1098-1136 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/49949 |
identifier_str_mv |
Belkacemi, Thabet; Niermann, Alexander; Hofmann, Laura; Wissenbach, Ulrich; Birnbaumer, Lutz; et al.; TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo; Wiley-liss, Div John Wiley & Sons Inc; Glia; 65; 9; 6-2017; 1535-1549 0894-1491 1098-1136 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1002/glia.23180 info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.23180 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-liss, Div John Wiley & Sons Inc |
publisher.none.fl_str_mv |
Wiley-liss, Div John Wiley & Sons Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842269910221193216 |
score |
13.13397 |