Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos

Autores
Sansiñena, Marina Julia; Santos, María Victoria; Taminelli, Guillermo; Zaritzky, Noemí
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Santos, María Victoria. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Taminelli, Guillermo. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Zaritzky, Noemí. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Abstract: Devitrification, the process of crystallization of a formerly crystal-free, amorphous glass state, can lead to damage during the warming of cells. The objective of this study was to determine the glass transition temperature of a cryopreservation solution typically used in the vitrification, storage and warming of mammalian oocytes and embryos using Differential Scanning Calorimetry. A numerical model of the heat transfer process to analyze warming and devitrification thresholds for a common vitrification carrier (open pulled straw, OPS) was conducted. The implications on specimen handling and storage inside the dewar in contact with nitrogen vapor phase at different temperatures were determined. The time required for initiation of devitrification of a vitrified sample was determined by mathematical modeling and compared with measured temperatures in the vapor phase of liquid nitrogen cryogenic dewars. Results indicated that the glass transition ranged from -126 to -121ºC and devitrification was initiated at -109ºC. Interestingly, samples entered rubbery state at -121ºC and therefore could potentially initiate devitrification above this value, with the consequent damaging effects to cell survival. Devitrification times were calculated considering an initial temperature of material immersed in liquid nitrogen (-196ºC) and two temperatures of liquid nitrogen vapors within the dewar (-50 and -70ºC) to which the sample could be exposed for a period of time, either during storage or upon its removal. The mathematical model indicated samples could reach glass transition temperatures and undergo devitrification in 30 seconds. Results of the present study indicate storage of vitrified oocytes and embryos in the liquid nitrogen vapor phase (as opposed to completely immersed in liquid nitrogen) poses the potential risk of devitrification. Due to the reduced time-handling period before samples reach critical rubbery and devitrification values, caution should be exercised when handling samples in vapor phase
Fuente
Theriogenology. 2014;82(3):373–378
Materia
VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/5495
Acceder
id RIUCA_d140bf8cbb1fcb7061e25d040517f915
oai_identifier_str oai:ucacris:123456789/5495
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryosSansiñena, Marina JuliaSantos, María VictoriaTaminelli, GuillermoZaritzky, NoemíVITRIFICACIONEMBRIONESNITROGENO LIQUIDOFil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; ArgentinaFil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; ArgentinaFil: Santos, María Victoria. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Taminelli, Guillermo. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; ArgentinaFil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; ArgentinaFil: Zaritzky, Noemí. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaAbstract: Devitrification, the process of crystallization of a formerly crystal-free, amorphous glass state, can lead to damage during the warming of cells. The objective of this study was to determine the glass transition temperature of a cryopreservation solution typically used in the vitrification, storage and warming of mammalian oocytes and embryos using Differential Scanning Calorimetry. A numerical model of the heat transfer process to analyze warming and devitrification thresholds for a common vitrification carrier (open pulled straw, OPS) was conducted. The implications on specimen handling and storage inside the dewar in contact with nitrogen vapor phase at different temperatures were determined. The time required for initiation of devitrification of a vitrified sample was determined by mathematical modeling and compared with measured temperatures in the vapor phase of liquid nitrogen cryogenic dewars. Results indicated that the glass transition ranged from -126 to -121ºC and devitrification was initiated at -109ºC. Interestingly, samples entered rubbery state at -121ºC and therefore could potentially initiate devitrification above this value, with the consequent damaging effects to cell survival. Devitrification times were calculated considering an initial temperature of material immersed in liquid nitrogen (-196ºC) and two temperatures of liquid nitrogen vapors within the dewar (-50 and -70ºC) to which the sample could be exposed for a period of time, either during storage or upon its removal. The mathematical model indicated samples could reach glass transition temperatures and undergo devitrification in 30 seconds. Results of the present study indicate storage of vitrified oocytes and embryos in the liquid nitrogen vapor phase (as opposed to completely immersed in liquid nitrogen) poses the potential risk of devitrification. Due to the reduced time-handling period before samples reach critical rubbery and devitrification values, caution should be exercised when handling samples in vapor phaseElsevier2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/54950093-691X10.1016/j.theriogenology.2014.04.003Sansinena M, Santos MV, Taminelli G, Zaritky N. Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos [en línea]. Theriogenology. 2014;82(3):373–378. doi:10.1016/j.theriogenology.2014.04.003 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/5495Theriogenology. 2014;82(3):373–378reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:56:07Zoai:ucacris:123456789/5495instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:56:07.975Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
title Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
spellingShingle Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
Sansiñena, Marina Julia
VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
title_short Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
title_full Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
title_fullStr Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
title_full_unstemmed Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
title_sort Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos
dc.creator.none.fl_str_mv Sansiñena, Marina Julia
Santos, María Victoria
Taminelli, Guillermo
Zaritzky, Noemí
author Sansiñena, Marina Julia
author_facet Sansiñena, Marina Julia
Santos, María Victoria
Taminelli, Guillermo
Zaritzky, Noemí
author_role author
author2 Santos, María Victoria
Taminelli, Guillermo
Zaritzky, Noemí
author2_role author
author
author
dc.subject.none.fl_str_mv VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
topic VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
dc.description.none.fl_txt_mv Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Santos, María Victoria. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Taminelli, Guillermo. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Zaritzky, Noemí. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Abstract: Devitrification, the process of crystallization of a formerly crystal-free, amorphous glass state, can lead to damage during the warming of cells. The objective of this study was to determine the glass transition temperature of a cryopreservation solution typically used in the vitrification, storage and warming of mammalian oocytes and embryos using Differential Scanning Calorimetry. A numerical model of the heat transfer process to analyze warming and devitrification thresholds for a common vitrification carrier (open pulled straw, OPS) was conducted. The implications on specimen handling and storage inside the dewar in contact with nitrogen vapor phase at different temperatures were determined. The time required for initiation of devitrification of a vitrified sample was determined by mathematical modeling and compared with measured temperatures in the vapor phase of liquid nitrogen cryogenic dewars. Results indicated that the glass transition ranged from -126 to -121ºC and devitrification was initiated at -109ºC. Interestingly, samples entered rubbery state at -121ºC and therefore could potentially initiate devitrification above this value, with the consequent damaging effects to cell survival. Devitrification times were calculated considering an initial temperature of material immersed in liquid nitrogen (-196ºC) and two temperatures of liquid nitrogen vapors within the dewar (-50 and -70ºC) to which the sample could be exposed for a period of time, either during storage or upon its removal. The mathematical model indicated samples could reach glass transition temperatures and undergo devitrification in 30 seconds. Results of the present study indicate storage of vitrified oocytes and embryos in the liquid nitrogen vapor phase (as opposed to completely immersed in liquid nitrogen) poses the potential risk of devitrification. Due to the reduced time-handling period before samples reach critical rubbery and devitrification values, caution should be exercised when handling samples in vapor phase
description Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/5495
0093-691X
10.1016/j.theriogenology.2014.04.003
Sansinena M, Santos MV, Taminelli G, Zaritky N. Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos [en línea]. Theriogenology. 2014;82(3):373–378. doi:10.1016/j.theriogenology.2014.04.003 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/5495
url https://repositorio.uca.edu.ar/handle/123456789/5495
identifier_str_mv 0093-691X
10.1016/j.theriogenology.2014.04.003
Sansinena M, Santos MV, Taminelli G, Zaritky N. Implications of storage and handling conditions on glass transition and potential devitrification of oocytes and embryos [en línea]. Theriogenology. 2014;82(3):373–378. doi:10.1016/j.theriogenology.2014.04.003 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/5495
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Theriogenology. 2014;82(3):373–378
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.221938

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