Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos

Autores
Sansiñena, Marina Julia; Zaritzky, Noemí; Taminelli, Guillermo; Santos, María Victoria
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Agrarias; Argentina
Fil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingeniería. Depto. de Ingeniería Química; Argentina
Fil: Zaritzky, Noemí. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Taminelli, Guillermo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Agrarias; Argentina
Fil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Santos, María Victoria. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: Devitrification, the process of crystallization of a formerly crystal-free, amorphous glass state, can lead to damage during the warming of cells. The objective of this study was to determine the glass transition temperature of a cryopreservation solution typically used in the vitrification, storage and warming of mammalian oocytes and embryos using Differential Scanning Calorimetry. A numerical model of the heat transfer process to analyze warming and devitrification thresholds for a vitrification carrier (open-pulled straw, OPS) was conducted and the implications on specimen storage in nitrogen vapor phase were determined. The time required for initiation of devitrification was determined by mathematical modeling and compared with temperatures in the vapor phase of liquid nitrogen cryogenic dewars. Results indicated that the glass transition ranged from -126 to -121ºC and devitrification was initiated at -109ºC. Interestingly, samples entered rubbery state at -121ºC and therefore could potentially initiate devitrification above this value, with the consequent damaging effects to cell survival. Devitrification itimes were mathematically modeled considering an initial temperature of material immersed in liquid nitrogen (-196ºC) and two arbitrarily selected temperatures (-50 and -70ºC) to which a sample could be exposed at the neck of dewar. The mathematical model indicated samples could reach glass transition temperatures and undergo devitrification in 30 seconds. Results of the present study indicate storage of vitrified oocytes and embryos in the liquid nitrogen vapor phase (as opposed to completely immersed in liquid nitrogen) poses the potential risk of devitrification. Due to the reduced time handling period before samples reach critical rubbery and devitrification values, caution should be exercised when handling samples in vapor phase
Fuente
Posprint del artículo publicado en Theriogenology, 2014
Materia
VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/5487
Acceder
id RIUCA_e7c888413c618e53e1c94de5b17d4dcb
oai_identifier_str oai:ucacris:123456789/5487
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryosSansiñena, Marina JuliaZaritzky, NoemíTaminelli, GuillermoSantos, María VictoriaVITRIFICACIONEMBRIONESNITROGENO LIQUIDOFil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Agrarias; ArgentinaFil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingeniería. Depto. de Ingeniería Química; ArgentinaFil: Zaritzky, Noemí. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Taminelli, Guillermo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Agrarias; ArgentinaFil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; ArgentinaFil: Santos, María Victoria. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAbstract: Devitrification, the process of crystallization of a formerly crystal-free, amorphous glass state, can lead to damage during the warming of cells. The objective of this study was to determine the glass transition temperature of a cryopreservation solution typically used in the vitrification, storage and warming of mammalian oocytes and embryos using Differential Scanning Calorimetry. A numerical model of the heat transfer process to analyze warming and devitrification thresholds for a vitrification carrier (open-pulled straw, OPS) was conducted and the implications on specimen storage in nitrogen vapor phase were determined. The time required for initiation of devitrification was determined by mathematical modeling and compared with temperatures in the vapor phase of liquid nitrogen cryogenic dewars. Results indicated that the glass transition ranged from -126 to -121ºC and devitrification was initiated at -109ºC. Interestingly, samples entered rubbery state at -121ºC and therefore could potentially initiate devitrification above this value, with the consequent damaging effects to cell survival. Devitrification itimes were mathematically modeled considering an initial temperature of material immersed in liquid nitrogen (-196ºC) and two arbitrarily selected temperatures (-50 and -70ºC) to which a sample could be exposed at the neck of dewar. The mathematical model indicated samples could reach glass transition temperatures and undergo devitrification in 30 seconds. Results of the present study indicate storage of vitrified oocytes and embryos in the liquid nitrogen vapor phase (as opposed to completely immersed in liquid nitrogen) poses the potential risk of devitrification. Due to the reduced time handling period before samples reach critical rubbery and devitrification values, caution should be exercised when handling samples in vapor phaseUniversidad Católica Argentina. Facultad de Ciencias AgrariasUniversidad Nacional de La Plata. Facultad de Ingeniería. Centro de Investigación y Desarrollo en Criotecnología de AlimentosCONICET2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/5487Sansinena, M., Santos, M. V., Taminelli, G. y N. Zaritky. 2014. Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embroyos [en línea]. Postprint del artículo publicado en Theriogenology: http//dx.doi.org/10.1016/j.theriogenology.2014.04.003 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/5487Posprint del artículo publicado en Theriogenology, 2014reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:56:07Zoai:ucacris:123456789/5487instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:56:07.952Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
title Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
spellingShingle Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
Sansiñena, Marina Julia
VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
title_short Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
title_full Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
title_fullStr Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
title_full_unstemmed Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
title_sort Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embryos
dc.creator.none.fl_str_mv Sansiñena, Marina Julia
Zaritzky, Noemí
Taminelli, Guillermo
Santos, María Victoria
author Sansiñena, Marina Julia
author_facet Sansiñena, Marina Julia
Zaritzky, Noemí
Taminelli, Guillermo
Santos, María Victoria
author_role author
author2 Zaritzky, Noemí
Taminelli, Guillermo
Santos, María Victoria
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad Católica Argentina. Facultad de Ciencias Agrarias
Universidad Nacional de La Plata. Facultad de Ingeniería. Centro de Investigación y Desarrollo en Criotecnología de Alimentos
CONICET
dc.subject.none.fl_str_mv VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
topic VITRIFICACION
EMBRIONES
NITROGENO LIQUIDO
dc.description.none.fl_txt_mv Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Agrarias; Argentina
Fil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingeniería. Depto. de Ingeniería Química; Argentina
Fil: Zaritzky, Noemí. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Taminelli, Guillermo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Agrarias; Argentina
Fil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Santos, María Victoria. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: Devitrification, the process of crystallization of a formerly crystal-free, amorphous glass state, can lead to damage during the warming of cells. The objective of this study was to determine the glass transition temperature of a cryopreservation solution typically used in the vitrification, storage and warming of mammalian oocytes and embryos using Differential Scanning Calorimetry. A numerical model of the heat transfer process to analyze warming and devitrification thresholds for a vitrification carrier (open-pulled straw, OPS) was conducted and the implications on specimen storage in nitrogen vapor phase were determined. The time required for initiation of devitrification was determined by mathematical modeling and compared with temperatures in the vapor phase of liquid nitrogen cryogenic dewars. Results indicated that the glass transition ranged from -126 to -121ºC and devitrification was initiated at -109ºC. Interestingly, samples entered rubbery state at -121ºC and therefore could potentially initiate devitrification above this value, with the consequent damaging effects to cell survival. Devitrification itimes were mathematically modeled considering an initial temperature of material immersed in liquid nitrogen (-196ºC) and two arbitrarily selected temperatures (-50 and -70ºC) to which a sample could be exposed at the neck of dewar. The mathematical model indicated samples could reach glass transition temperatures and undergo devitrification in 30 seconds. Results of the present study indicate storage of vitrified oocytes and embryos in the liquid nitrogen vapor phase (as opposed to completely immersed in liquid nitrogen) poses the potential risk of devitrification. Due to the reduced time handling period before samples reach critical rubbery and devitrification values, caution should be exercised when handling samples in vapor phase
description Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Agrarias; Argentina
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/5487
Sansinena, M., Santos, M. V., Taminelli, G. y N. Zaritky. 2014. Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embroyos [en línea]. Postprint del artículo publicado en Theriogenology: http//dx.doi.org/10.1016/j.theriogenology.2014.04.003 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/5487
url https://repositorio.uca.edu.ar/handle/123456789/5487
identifier_str_mv Sansinena, M., Santos, M. V., Taminelli, G. y N. Zaritky. 2014. Implications of glass transition in the devitrification process and storage management of vitrified oocytes and embroyos [en línea]. Postprint del artículo publicado en Theriogenology: http//dx.doi.org/10.1016/j.theriogenology.2014.04.003 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/5487
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Posprint del artículo publicado en Theriogenology, 2014
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.13397

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