In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage...

Autores
Sansiñena, Marina Julia; Santos, María Victoria; Chirife, Jorge; Zaritzky, Noemí
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chirife, Jorge. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zaritzky, Noemí. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Abstract: Heat transfer during cooling and warming is difficult to measure in cryo-devices; mathematical modelling is an alternative method that can describe these processes. In this study, we tested the validity of one such model by assessing in-vitro development of vitrified and warmed bovine oocytes after parthenogenetic activation and culture. The viability of oocytes vitrified in four different cryo-devices was assessed. Consistent with modelling predictions, oocytes vitrified using cryo-devices with the highest modelled cooling rates had significantly (P < 0.05) better cleavage and blastocyst formation rates. We then evaluated a two-step sample removal process, in which oocytes were held in nitrogen vapour for 15 s to simulate sample identification during clinical application, before being removed completely and warmed. Oocytes exposed to this procedure showed reduced developmental potential, according to the model, owing to thermodynamic instability and devitrification at relatively low temperatures. These findings suggest that cryo-device selection and handling, including method of removal from nitrogen storage, are critical to survival of vitrified oocytes. Limitations of the study include use of parthenogenetically activated rather than fertilized ova and lack of physical measurement of recrystallization. We suggest mathematical modelling could be used to predict the effect of critical steps in cryopreservation.
Fuente
Reproductive BioMedicine. 2018, 36(5)
Materia
FERTILIZACION IN VITRO
TRANSFERENCIA DE CALOR
DESARROLLO EMBRIONARIO
CRIOPRESERVACION
VITRIFICACION
OVOCITOS
BOVINOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/9493
Acceder
id RIUCA_bff1589edf6e71ee6321b227a502dabb
oai_identifier_str oai:ucacris:123456789/9493
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removalSansiñena, Marina JuliaSantos, María VictoriaChirife, JorgeZaritzky, NoemíFERTILIZACION IN VITROTRANSFERENCIA DE CALORDESARROLLO EMBRIONARIOCRIOPRESERVACIONVITRIFICACIONOVOCITOSBOVINOSFil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; ArgentinaFil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; ArgentinaFil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chirife, Jorge. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; ArgentinaFil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; ArgentinaFil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zaritzky, Noemí. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; ArgentinaAbstract: Heat transfer during cooling and warming is difficult to measure in cryo-devices; mathematical modelling is an alternative method that can describe these processes. In this study, we tested the validity of one such model by assessing in-vitro development of vitrified and warmed bovine oocytes after parthenogenetic activation and culture. The viability of oocytes vitrified in four different cryo-devices was assessed. Consistent with modelling predictions, oocytes vitrified using cryo-devices with the highest modelled cooling rates had significantly (P < 0.05) better cleavage and blastocyst formation rates. We then evaluated a two-step sample removal process, in which oocytes were held in nitrogen vapour for 15 s to simulate sample identification during clinical application, before being removed completely and warmed. Oocytes exposed to this procedure showed reduced developmental potential, according to the model, owing to thermodynamic instability and devitrification at relatively low temperatures. These findings suggest that cryo-device selection and handling, including method of removal from nitrogen storage, are critical to survival of vitrified oocytes. Limitations of the study include use of parthenogenetically activated rather than fertilized ova and lack of physical measurement of recrystallization. We suggest mathematical modelling could be used to predict the effect of critical steps in cryopreservation.Elsevier2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/94931472-6483 (impreso)1472-6491 (online)10.1016/j.rbmo.2018.01.00329422429Sansiñena,M.J. et. al. In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal [en línea]. Reproductive BioMedicine. 2018, 36(5). doi:10.1016/j.rbmo.2018.01.003 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9493Reproductive BioMedicine. 2018, 36(5)reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:57:09Zoai:ucacris:123456789/9493instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:57:09.318Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
spellingShingle In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
Sansiñena, Marina Julia
FERTILIZACION IN VITRO
TRANSFERENCIA DE CALOR
DESARROLLO EMBRIONARIO
CRIOPRESERVACION
VITRIFICACION
OVOCITOS
BOVINOS
title_short In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_full In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_fullStr In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_full_unstemmed In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
title_sort In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
dc.creator.none.fl_str_mv Sansiñena, Marina Julia
Santos, María Victoria
Chirife, Jorge
Zaritzky, Noemí
author Sansiñena, Marina Julia
author_facet Sansiñena, Marina Julia
Santos, María Victoria
Chirife, Jorge
Zaritzky, Noemí
author_role author
author2 Santos, María Victoria
Chirife, Jorge
Zaritzky, Noemí
author2_role author
author
author
dc.subject.none.fl_str_mv FERTILIZACION IN VITRO
TRANSFERENCIA DE CALOR
DESARROLLO EMBRIONARIO
CRIOPRESERVACION
VITRIFICACION
OVOCITOS
BOVINOS
topic FERTILIZACION IN VITRO
TRANSFERENCIA DE CALOR
DESARROLLO EMBRIONARIO
CRIOPRESERVACION
VITRIFICACION
OVOCITOS
BOVINOS
dc.description.none.fl_txt_mv Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Santos, María Victoria. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Santos, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chirife, Jorge. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Fil: Zaritzky, Noemí. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; Argentina
Fil: Zaritzky, Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zaritzky, Noemí. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
Abstract: Heat transfer during cooling and warming is difficult to measure in cryo-devices; mathematical modelling is an alternative method that can describe these processes. In this study, we tested the validity of one such model by assessing in-vitro development of vitrified and warmed bovine oocytes after parthenogenetic activation and culture. The viability of oocytes vitrified in four different cryo-devices was assessed. Consistent with modelling predictions, oocytes vitrified using cryo-devices with the highest modelled cooling rates had significantly (P < 0.05) better cleavage and blastocyst formation rates. We then evaluated a two-step sample removal process, in which oocytes were held in nitrogen vapour for 15 s to simulate sample identification during clinical application, before being removed completely and warmed. Oocytes exposed to this procedure showed reduced developmental potential, according to the model, owing to thermodynamic instability and devitrification at relatively low temperatures. These findings suggest that cryo-device selection and handling, including method of removal from nitrogen storage, are critical to survival of vitrified oocytes. Limitations of the study include use of parthenogenetically activated rather than fertilized ova and lack of physical measurement of recrystallization. We suggest mathematical modelling could be used to predict the effect of critical steps in cryopreservation.
description Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias; Argentina
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/9493
1472-6483 (impreso)
1472-6491 (online)
10.1016/j.rbmo.2018.01.003
29422429
Sansiñena,M.J. et. al. In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal [en línea]. Reproductive BioMedicine. 2018, 36(5). doi:10.1016/j.rbmo.2018.01.003 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9493
url https://repositorio.uca.edu.ar/handle/123456789/9493
identifier_str_mv 1472-6483 (impreso)
1472-6491 (online)
10.1016/j.rbmo.2018.01.003
29422429
Sansiñena,M.J. et. al. In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal [en línea]. Reproductive BioMedicine. 2018, 36(5). doi:10.1016/j.rbmo.2018.01.003 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9493
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Reproductive BioMedicine. 2018, 36(5)
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
_version_ 1836638349826195456
score 13.13397

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