Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528

Autores
Lario, Luciana Daniela; Pillaca-Pullo, Omar Santiago; Durães Sette, Lara; Converti, Attilio; Casati, Paula; Spampinato, Claudia; Pessoa, Adalberto
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Fil: Lario, Luciana Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Lario, Luciana Daniela. Universidad de Sao Paulo. Escuela de Ciencias Farmacéuticas. Departamento de Tecnología Bioquímica y Farmacéutica; Brasil
Fil: Lario, Luciana Daniela. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Instituto de Ingeniería Ambiental, Química y Biotecnología Aplicada; Argentina
Fil: Pillaca-Pullo, Omar Santiago. Universidad de Sao Paulo. Escuela de Ciencias Farmacéuticas. Departamento de Tecnología Bioquímica y Farmacéutica; Brasil
Fil: Durães Sette, Lara. Universidad Estatal Paulista. Instituto de Biociencias. Departamento de Biología General y Aplicada; Brasil
Fil: Converti, Attilio. Universidad de Génova. Polo de Química Ingeniería. Departamento de Ingeniería Civil, Química y Ambiental; Italia
Fil: Casati, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Spampinato, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Pessoa, Adalberto. Universidad de Sao Paulo. Escuela de Ciencias Farmacéuticas. Departamento de Tecnología Bioquímica y Farmacéutica; Brasil20
Abstract: Enzymes from cold-adapted microorganisms are of high interest to industries due to their high activity at low and mild temperatures, which makes them suitable for their use in several processes that either require a supply of exogenous energy or involve the use of heat labile products. In this work, the protease production by the strain Rhodotorula mucilaginosa CBMAI 1528, previously isolated from the Antarctic continent, was optimized, and the purified enzyme analyzed. It was found that protease production was dependent on culture medium composition and growth temperature, being 20 ºC and a culture medium containing both glucose and casein peptone (20 and 10 g/L, respectively) the optimal growing conditions in batch as well as in bioreactor.
Fuente
Biotechnology Reports Nro. 28 Año 2020
Materia
PROTEASAS
ENZIMAS
LEVADURA
ESPECTOMETRIA DE MASAS
AMINOACIDOS
REACCION QUIMICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/10930
Acceder
id RIUCA_7b8e06e6df13a238041c1e347805cb5d
oai_identifier_str oai:ucacris:123456789/10930
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528Lario, Luciana DanielaPillaca-Pullo, Omar SantiagoDurães Sette, LaraConverti, AttilioCasati, PaulaSpampinato, ClaudiaPessoa, AdalbertoPROTEASASENZIMASLEVADURAESPECTOMETRIA DE MASASAMINOACIDOSREACCION QUIMICAFil: Lario, Luciana Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Lario, Luciana Daniela. Universidad de Sao Paulo. Escuela de Ciencias Farmacéuticas. Departamento de Tecnología Bioquímica y Farmacéutica; BrasilFil: Lario, Luciana Daniela. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Instituto de Ingeniería Ambiental, Química y Biotecnología Aplicada; ArgentinaFil: Pillaca-Pullo, Omar Santiago. Universidad de Sao Paulo. Escuela de Ciencias Farmacéuticas. Departamento de Tecnología Bioquímica y Farmacéutica; BrasilFil: Durães Sette, Lara. Universidad Estatal Paulista. Instituto de Biociencias. Departamento de Biología General y Aplicada; BrasilFil: Converti, Attilio. Universidad de Génova. Polo de Química Ingeniería. Departamento de Ingeniería Civil, Química y Ambiental; ItaliaFil: Casati, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Spampinato, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Pessoa, Adalberto. Universidad de Sao Paulo. Escuela de Ciencias Farmacéuticas. Departamento de Tecnología Bioquímica y Farmacéutica; Brasil20Abstract: Enzymes from cold-adapted microorganisms are of high interest to industries due to their high activity at low and mild temperatures, which makes them suitable for their use in several processes that either require a supply of exogenous energy or involve the use of heat labile products. In this work, the protease production by the strain Rhodotorula mucilaginosa CBMAI 1528, previously isolated from the Antarctic continent, was optimized, and the purified enzyme analyzed. It was found that protease production was dependent on culture medium composition and growth temperature, being 20 ºC and a culture medium containing both glucose and casein peptone (20 and 10 g/L, respectively) the optimal growing conditions in batch as well as in bioreactor.Elsevier B. V.2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/109302215-017X10.1016/j.btre.2020.e00546Lario, L. D. [et al.] Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528 [en línea] Biotechnology Reports. 2020, 28. Doi: 10.1016/j.btre.2020.e00546 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10930Biotechnology Reports Nro. 28 Año 2020reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:57:35Zoai:ucacris:123456789/10930instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:57:36.146Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
title Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
spellingShingle Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
Lario, Luciana Daniela
PROTEASAS
ENZIMAS
LEVADURA
ESPECTOMETRIA DE MASAS
AMINOACIDOS
REACCION QUIMICA
title_short Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
title_full Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
title_fullStr Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
title_full_unstemmed Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
title_sort Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528
dc.creator.none.fl_str_mv Lario, Luciana Daniela
Pillaca-Pullo, Omar Santiago
Durães Sette, Lara
Converti, Attilio
Casati, Paula
Spampinato, Claudia
Pessoa, Adalberto
author Lario, Luciana Daniela
author_facet Lario, Luciana Daniela
Pillaca-Pullo, Omar Santiago
Durães Sette, Lara
Converti, Attilio
Casati, Paula
Spampinato, Claudia
Pessoa, Adalberto
author_role author
author2 Pillaca-Pullo, Omar Santiago
Durães Sette, Lara
Converti, Attilio
Casati, Paula
Spampinato, Claudia
Pessoa, Adalberto
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PROTEASAS
ENZIMAS
LEVADURA
ESPECTOMETRIA DE MASAS
AMINOACIDOS
REACCION QUIMICA
topic PROTEASAS
ENZIMAS
LEVADURA
ESPECTOMETRIA DE MASAS
AMINOACIDOS
REACCION QUIMICA
dc.description.none.fl_txt_mv Fil: Lario, Luciana Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Lario, Luciana Daniela. Universidad de Sao Paulo. Escuela de Ciencias Farmacéuticas. Departamento de Tecnología Bioquímica y Farmacéutica; Brasil
Fil: Lario, Luciana Daniela. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Instituto de Ingeniería Ambiental, Química y Biotecnología Aplicada; Argentina
Fil: Pillaca-Pullo, Omar Santiago. Universidad de Sao Paulo. Escuela de Ciencias Farmacéuticas. Departamento de Tecnología Bioquímica y Farmacéutica; Brasil
Fil: Durães Sette, Lara. Universidad Estatal Paulista. Instituto de Biociencias. Departamento de Biología General y Aplicada; Brasil
Fil: Converti, Attilio. Universidad de Génova. Polo de Química Ingeniería. Departamento de Ingeniería Civil, Química y Ambiental; Italia
Fil: Casati, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Spampinato, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Pessoa, Adalberto. Universidad de Sao Paulo. Escuela de Ciencias Farmacéuticas. Departamento de Tecnología Bioquímica y Farmacéutica; Brasil20
Abstract: Enzymes from cold-adapted microorganisms are of high interest to industries due to their high activity at low and mild temperatures, which makes them suitable for their use in several processes that either require a supply of exogenous energy or involve the use of heat labile products. In this work, the protease production by the strain Rhodotorula mucilaginosa CBMAI 1528, previously isolated from the Antarctic continent, was optimized, and the purified enzyme analyzed. It was found that protease production was dependent on culture medium composition and growth temperature, being 20 ºC and a culture medium containing both glucose and casein peptone (20 and 10 g/L, respectively) the optimal growing conditions in batch as well as in bioreactor.
description Fil: Lario, Luciana Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/10930
2215-017X
10.1016/j.btre.2020.e00546
Lario, L. D. [et al.] Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528 [en línea] Biotechnology Reports. 2020, 28. Doi: 10.1016/j.btre.2020.e00546 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10930
url https://repositorio.uca.edu.ar/handle/123456789/10930
identifier_str_mv 2215-017X
10.1016/j.btre.2020.e00546
Lario, L. D. [et al.] Optimization of protease production and sequence analysis of the purified enzyme from the cold adapted yeast Rhodotorula mucilaginosa CBMAI 1528 [en línea] Biotechnology Reports. 2020, 28. Doi: 10.1016/j.btre.2020.e00546 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10930
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B. V.
publisher.none.fl_str_mv Elsevier B. V.
dc.source.none.fl_str_mv Biotechnology Reports Nro. 28 Año 2020
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.22299

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