Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor

Autores
Machado, Suellen; Feitosa, Valker; Pillaca Pullo, Omar; Lario, Luciana Daniela; Sette, Lara; Pessoa, Adalberto; Alves, Harley
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72 h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment.
Fil: Machado, Suellen. Universidade Estadual Da Paraiba.; Brasil. Universidade de Sao Paulo; Brasil. Universidade Federal Do Para. Instituto de Ciencias Biologicas; Brasil
Fil: Feitosa, Valker. Universidade de Sao Paulo; Brasil. Universidade Federal de Viçosa; Brasil
Fil: Pillaca Pullo, Omar. Universidad Privada Norbert Wiener; Perú
Fil: Lario, Luciana Daniela. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Química e Ingeniería del Rosario. Departamento de Investigación Institucional; Argentina. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Sette, Lara. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Pessoa, Adalberto. Universidade de Sao Paulo; Brasil
Fil: Alves, Harley. Universidade Estadual Da Paraiba.; Brasil
Materia
BIOREACTOR
EXTREMOPHILIC
OXYGEN TRANSFERENCE
PROTEINASE
PROTEOLYTIC
PSYCHROPHILIC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/218519
Acceder
id CONICETDig_4225e2fb563957bc086b61e7cb884b4d
oai_identifier_str oai:ri.conicet.gov.ar:11336/218519
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank BioreactorMachado, SuellenFeitosa, ValkerPillaca Pullo, OmarLario, Luciana DanielaSette, LaraPessoa, AdalbertoAlves, HarleyBIOREACTOREXTREMOPHILICOXYGEN TRANSFERENCEPROTEINASEPROTEOLYTICPSYCHROPHILIChttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72 h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment.Fil: Machado, Suellen. Universidade Estadual Da Paraiba.; Brasil. Universidade de Sao Paulo; Brasil. Universidade Federal Do Para. Instituto de Ciencias Biologicas; BrasilFil: Feitosa, Valker. Universidade de Sao Paulo; Brasil. Universidade Federal de Viçosa; BrasilFil: Pillaca Pullo, Omar. Universidad Privada Norbert Wiener; PerúFil: Lario, Luciana Daniela. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Química e Ingeniería del Rosario. Departamento de Investigación Institucional; Argentina. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Sette, Lara. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Pessoa, Adalberto. Universidade de Sao Paulo; BrasilFil: Alves, Harley. Universidade Estadual Da Paraiba.; BrasilMultidisciplinary Digital Publishing Institute2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/218519Machado, Suellen; Feitosa, Valker; Pillaca Pullo, Omar; Lario, Luciana Daniela; Sette, Lara; et al.; Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor; Multidisciplinary Digital Publishing Institute; Bioengineering; 9; 11; 11-2022; 1-142306-5354CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2306-5354/9/11/694info:eu-repo/semantics/altIdentifier/doi/10.3390/bioengineering9110694info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:10:38Zoai:ri.conicet.gov.ar:11336/218519instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:10:38.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
title Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
spellingShingle Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
Machado, Suellen
BIOREACTOR
EXTREMOPHILIC
OXYGEN TRANSFERENCE
PROTEINASE
PROTEOLYTIC
PSYCHROPHILIC
title_short Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
title_full Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
title_fullStr Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
title_full_unstemmed Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
title_sort Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor
dc.creator.none.fl_str_mv Machado, Suellen
Feitosa, Valker
Pillaca Pullo, Omar
Lario, Luciana Daniela
Sette, Lara
Pessoa, Adalberto
Alves, Harley
author Machado, Suellen
author_facet Machado, Suellen
Feitosa, Valker
Pillaca Pullo, Omar
Lario, Luciana Daniela
Sette, Lara
Pessoa, Adalberto
Alves, Harley
author_role author
author2 Feitosa, Valker
Pillaca Pullo, Omar
Lario, Luciana Daniela
Sette, Lara
Pessoa, Adalberto
Alves, Harley
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOREACTOR
EXTREMOPHILIC
OXYGEN TRANSFERENCE
PROTEINASE
PROTEOLYTIC
PSYCHROPHILIC
topic BIOREACTOR
EXTREMOPHILIC
OXYGEN TRANSFERENCE
PROTEINASE
PROTEOLYTIC
PSYCHROPHILIC
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72 h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment.
Fil: Machado, Suellen. Universidade Estadual Da Paraiba.; Brasil. Universidade de Sao Paulo; Brasil. Universidade Federal Do Para. Instituto de Ciencias Biologicas; Brasil
Fil: Feitosa, Valker. Universidade de Sao Paulo; Brasil. Universidade Federal de Viçosa; Brasil
Fil: Pillaca Pullo, Omar. Universidad Privada Norbert Wiener; Perú
Fil: Lario, Luciana Daniela. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Facultad de Química e Ingeniería del Rosario. Departamento de Investigación Institucional; Argentina. Universidade de Sao Paulo; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Sette, Lara. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Pessoa, Adalberto. Universidade de Sao Paulo; Brasil
Fil: Alves, Harley. Universidade Estadual Da Paraiba.; Brasil
description Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72 h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment.
publishDate 2022
dc.date.none.fl_str_mv 2022-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/218519
Machado, Suellen; Feitosa, Valker; Pillaca Pullo, Omar; Lario, Luciana Daniela; Sette, Lara; et al.; Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor; Multidisciplinary Digital Publishing Institute; Bioengineering; 9; 11; 11-2022; 1-14
2306-5354
CONICET Digital
CONICET
url http://hdl.handle.net/11336/218519
identifier_str_mv Machado, Suellen; Feitosa, Valker; Pillaca Pullo, Omar; Lario, Luciana Daniela; Sette, Lara; et al.; Effects of Oxygen Transference on Protease Production by Rhodotorula mucilaginosa CBMAI 1528 in a Stirred Tank Bioreactor; Multidisciplinary Digital Publishing Institute; Bioengineering; 9; 11; 11-2022; 1-14
2306-5354
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2306-5354/9/11/694
info:eu-repo/semantics/altIdentifier/doi/10.3390/bioengineering9110694
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.004268

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