Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor

Autores
Machado, Suellen; Feitosa, Valker; Pillaca Pullo, Omar Santiago; Lario, Luciana Daniela; Durães Sette, Lara; Pessoa, Adalberto; Alves, Harley
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Machado, Suellen. Universidade Estadual da Paraíba. Departamento de Farmácia. Programa de Pós‐Graduação em Ciências Farmacêuticas; Brasil
Fil: Machado, Suellen. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; Brasil
Fil: Machado, Suellen. Universidade Federal do Pará. Faculdade de Biomedicina. Instituto de Ciências Biológicas; Brasil
Fil: Feitosa, Valker. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; Brasil
Fil: Feitosa, Valker. Universidade Federal de Viçosa. Departamento de Medicina e Enfermagem; Brasil
Fil: Pillaca Pullo, Omar Santiago. Universidad Privada Norbert Wiener. Centro de Investigación en Biodiversidad para la Salud; Perú
Fil: Lario, Luciana D. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; Brasil
Fil: Lario, Luciana D. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Instituto de Ingeniería Ambiental, Química y Biotecnología Aplicada; Argentina
Fil: Durães Sette, Lara. Universidade Estadual Paulista Júlio de Mesquita Filho. Departamento de Biologia Geral e Aplicada; Brasil
Fil: Pessoa, Adalberto. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; Brasil
Fil: Alves, Harley. Universidade Estadual da Paraíba. Departamento de Farmácia. Programa de Pós‐Graduação em Ciências Farmacêuticas; Brasil
Abstract: Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment.
Fuente
Bioengineering. Vol.9, No.11, 2022
Materia
EXTREMOFILO
PSICOFILO
PROTEINASA
PROTEOLITICO
BIORREACTOR
TRANSFERENCIA DE OXIGENO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/16424
Acceder
id RIUCA_81d21be5e203d1143ee17ef42e953911
oai_identifier_str oai:ucacris:123456789/16424
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactorMachado, SuellenFeitosa, ValkerPillaca Pullo, Omar SantiagoLario, Luciana DanielaDurães Sette, LaraPessoa, AdalbertoAlves, HarleyEXTREMOFILOPSICOFILOPROTEINASAPROTEOLITICOBIORREACTORTRANSFERENCIA DE OXIGENOFil: Machado, Suellen. Universidade Estadual da Paraíba. Departamento de Farmácia. Programa de Pós‐Graduação em Ciências Farmacêuticas; BrasilFil: Machado, Suellen. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; BrasilFil: Machado, Suellen. Universidade Federal do Pará. Faculdade de Biomedicina. Instituto de Ciências Biológicas; BrasilFil: Feitosa, Valker. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; BrasilFil: Feitosa, Valker. Universidade Federal de Viçosa. Departamento de Medicina e Enfermagem; BrasilFil: Pillaca Pullo, Omar Santiago. Universidad Privada Norbert Wiener. Centro de Investigación en Biodiversidad para la Salud; PerúFil: Lario, Luciana D. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; BrasilFil: Lario, Luciana D. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Instituto de Ingeniería Ambiental, Química y Biotecnología Aplicada; ArgentinaFil: Durães Sette, Lara. Universidade Estadual Paulista Júlio de Mesquita Filho. Departamento de Biologia Geral e Aplicada; BrasilFil: Pessoa, Adalberto. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; BrasilFil: Alves, Harley. Universidade Estadual da Paraíba. Departamento de Farmácia. Programa de Pós‐Graduação em Ciências Farmacêuticas; BrasilAbstract: Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment.MDPI AG2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/164242306-5354 (online)10.3390/bioengineering9110694Machado, S. et al. Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor [en línea]. Bioengineering. 2022, 9 (11). doi: 10.3390/bioengineering9110694. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16424Bioengineering. Vol.9, No.11, 2022reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:59:17Zoai:ucacris:123456789/16424instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:59:17.933Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor
title Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor
spellingShingle Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor
Machado, Suellen
EXTREMOFILO
PSICOFILO
PROTEINASA
PROTEOLITICO
BIORREACTOR
TRANSFERENCIA DE OXIGENO
title_short Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor
title_full Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor
title_fullStr Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor
title_full_unstemmed Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor
title_sort Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor
dc.creator.none.fl_str_mv Machado, Suellen
Feitosa, Valker
Pillaca Pullo, Omar Santiago
Lario, Luciana Daniela
Durães Sette, Lara
Pessoa, Adalberto
Alves, Harley
author Machado, Suellen
author_facet Machado, Suellen
Feitosa, Valker
Pillaca Pullo, Omar Santiago
Lario, Luciana Daniela
Durães Sette, Lara
Pessoa, Adalberto
Alves, Harley
author_role author
author2 Feitosa, Valker
Pillaca Pullo, Omar Santiago
Lario, Luciana Daniela
Durães Sette, Lara
Pessoa, Adalberto
Alves, Harley
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv EXTREMOFILO
PSICOFILO
PROTEINASA
PROTEOLITICO
BIORREACTOR
TRANSFERENCIA DE OXIGENO
topic EXTREMOFILO
PSICOFILO
PROTEINASA
PROTEOLITICO
BIORREACTOR
TRANSFERENCIA DE OXIGENO
dc.description.none.fl_txt_mv Fil: Machado, Suellen. Universidade Estadual da Paraíba. Departamento de Farmácia. Programa de Pós‐Graduação em Ciências Farmacêuticas; Brasil
Fil: Machado, Suellen. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; Brasil
Fil: Machado, Suellen. Universidade Federal do Pará. Faculdade de Biomedicina. Instituto de Ciências Biológicas; Brasil
Fil: Feitosa, Valker. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; Brasil
Fil: Feitosa, Valker. Universidade Federal de Viçosa. Departamento de Medicina e Enfermagem; Brasil
Fil: Pillaca Pullo, Omar Santiago. Universidad Privada Norbert Wiener. Centro de Investigación en Biodiversidad para la Salud; Perú
Fil: Lario, Luciana D. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; Brasil
Fil: Lario, Luciana D. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Instituto de Ingeniería Ambiental, Química y Biotecnología Aplicada; Argentina
Fil: Durães Sette, Lara. Universidade Estadual Paulista Júlio de Mesquita Filho. Departamento de Biologia Geral e Aplicada; Brasil
Fil: Pessoa, Adalberto. Universidade de São Paulo. Departamento de Tecnologia Bioquímico‐Farmacêutica; Brasil
Fil: Alves, Harley. Universidade Estadual da Paraíba. Departamento de Farmácia. Programa de Pós‐Graduação em Ciências Farmacêuticas; Brasil
Abstract: Microbial proteases, especially aspartic proteases, are an essential group of enzymes produced from different microorganisms. Microbial proteases have several applications, mainly in the food, beverage, cosmetic, and pharmaceutical industries, due to their efficiency in the processing and in the manufacturing stages. The yeast Rhodotorula mucilaginosa CBMAI 1528 was isolated from the Antarctic environment and was previously reported to have higher extracellular aspartic protease production. In addition, advances in the operational conditions of bioreactors for enzyme production are important to reduce the gap associated with scaling−up processes. This is the first study that evaluates the influence of oxygen transference (kLa) on the protease production of R. mucilaginosa yeast. To that end, batch cultures were created in a stirred tank bioreactor using Sabouraud dextrose broth at 25 °C for 72h under kLa values from 18 to 135 h−1. The results show that kLa (121 h−1) obtained at 500 rpm and 1.5 vvm plays an important role in protease production (124.9 U/mL) and productivity (6.784 U/L.h) as well as biomass (10.4 g/L), μmax (0.14 h−1) and Yx/s (0.484 g/g). In conclusion, R. mucilaginosa showed high yield production in aerobic culture with the efficiency of protease expression and secretion influenced by kLa. In this sense, our results could be used for further industrial investment.
description Fil: Machado, Suellen. Universidade Estadual da Paraíba. Departamento de Farmácia. Programa de Pós‐Graduação em Ciências Farmacêuticas; Brasil
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/16424
2306-5354 (online)
10.3390/bioengineering9110694
Machado, S. et al. Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor [en línea]. Bioengineering. 2022, 9 (11). doi: 10.3390/bioengineering9110694. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16424
url https://repositorio.uca.edu.ar/handle/123456789/16424
identifier_str_mv 2306-5354 (online)
10.3390/bioengineering9110694
Machado, S. et al. Effects of oxygen transference on protease production by rhodotorula mucilaginosa cbmai 1528 in a stirred tank bioreactor [en línea]. Bioengineering. 2022, 9 (11). doi: 10.3390/bioengineering9110694. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/16424
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv Bioengineering. Vol.9, No.11, 2022
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
_version_ 1836638368630308864
score 13.13397

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