Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis

Autores
Sterle, Helena Andrea; Valli, Eduardo; Cayrol, María Florencia; Paulazo, Maria Alejandra; Martinel Lamas, Diego J.; Díaz Flaqué, María Celeste; Klecha, Alicia Juana; Colombo, Lucas Luis; Medina, Vanina Araceli; Cremaschi, Graciela A.; Barreiro Arcos, María Laura
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Sterle, Helena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cayrol, María Florencia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cayrol, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paulazo, Maria Alejandra. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinel Lamas, Diego J. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotópos; Argentina
Fil: Colombo, Lucas Luis. Universidad de Buenos Aires. Instituto de Oncología Angel H. Roffo. Area de Investigación; Argentina
Fil: Colombo, Lucas Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotópos; Argentina
Fil: Cremaschi, Graciela A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cremaschi, Graciela A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotópos; Argentina
Fil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Barreiro Arcos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barreiro Arcos, María Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Abstract: We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development have been studied, but the results are still controversial. Herein, we show the modulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistent with the rate of cell division determined by staining tumor cells with carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly from the euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increased in hypothyroid mice. Intratumoral and peritumoral vasculogenesis was increased only in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis.
Fuente
Journal of Endocrinology. 2014;222(2):243-255
Materia
APOPTOSIS
ANGIOGENESIS
HORMONAS
GLANDULA TIROIDES
HIPOTIROIDISMO
TUMORES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/8787
Acceder
id RIUCA_7acfd9041238523b93080df001df6929
oai_identifier_str oai:ucacris:123456789/8787
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesisSterle, Helena AndreaValli, EduardoCayrol, María FlorenciaPaulazo, Maria AlejandraMartinel Lamas, Diego J.Díaz Flaqué, María CelesteKlecha, Alicia JuanaColombo, Lucas LuisMedina, Vanina AraceliCremaschi, Graciela A.Barreiro Arcos, María LauraAPOPTOSISANGIOGENESISHORMONASGLANDULA TIROIDESHIPOTIROIDISMOTUMORESFil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Sterle, Helena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cayrol, María Florencia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Cayrol, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Paulazo, Maria Alejandra. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinel Lamas, Diego J. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotópos; ArgentinaFil: Colombo, Lucas Luis. Universidad de Buenos Aires. Instituto de Oncología Angel H. Roffo. Area de Investigación; ArgentinaFil: Colombo, Lucas Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotópos; ArgentinaFil: Cremaschi, Graciela A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Cremaschi, Graciela A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cremaschi, Graciela A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotópos; ArgentinaFil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Barreiro Arcos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barreiro Arcos, María Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaAbstract: We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development have been studied, but the results are still controversial. Herein, we show the modulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistent with the rate of cell division determined by staining tumor cells with carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly from the euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increased in hypothyroid mice. Intratumoral and peritumoral vasculogenesis was increased only in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis.BioScientifica2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/87870022-0795 (impreso)1479-6805 (online)10.1530/JOE-14-015924928937Sterle HA, Valli E, Cayrol F, et al. Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis [en línea]. Journal of Endocrinology. 2014;222(2):243-255. doi:10.1530/JOE-14-0159 Registro en: https://repositorio.uca.edu.ar/handle/123456789/8787Journal of Endocrinology. 2014;222(2):243-255reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:56:56Zoai:ucacris:123456789/8787instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:56:57.05Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
spellingShingle Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
Sterle, Helena Andrea
APOPTOSIS
ANGIOGENESIS
HORMONAS
GLANDULA TIROIDES
HIPOTIROIDISMO
TUMORES
title_short Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_full Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_fullStr Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_full_unstemmed Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_sort Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
dc.creator.none.fl_str_mv Sterle, Helena Andrea
Valli, Eduardo
Cayrol, María Florencia
Paulazo, Maria Alejandra
Martinel Lamas, Diego J.
Díaz Flaqué, María Celeste
Klecha, Alicia Juana
Colombo, Lucas Luis
Medina, Vanina Araceli
Cremaschi, Graciela A.
Barreiro Arcos, María Laura
author Sterle, Helena Andrea
author_facet Sterle, Helena Andrea
Valli, Eduardo
Cayrol, María Florencia
Paulazo, Maria Alejandra
Martinel Lamas, Diego J.
Díaz Flaqué, María Celeste
Klecha, Alicia Juana
Colombo, Lucas Luis
Medina, Vanina Araceli
Cremaschi, Graciela A.
Barreiro Arcos, María Laura
author_role author
author2 Valli, Eduardo
Cayrol, María Florencia
Paulazo, Maria Alejandra
Martinel Lamas, Diego J.
Díaz Flaqué, María Celeste
Klecha, Alicia Juana
Colombo, Lucas Luis
Medina, Vanina Araceli
Cremaschi, Graciela A.
Barreiro Arcos, María Laura
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOPTOSIS
ANGIOGENESIS
HORMONAS
GLANDULA TIROIDES
HIPOTIROIDISMO
TUMORES
topic APOPTOSIS
ANGIOGENESIS
HORMONAS
GLANDULA TIROIDES
HIPOTIROIDISMO
TUMORES
dc.description.none.fl_txt_mv Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Sterle, Helena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cayrol, María Florencia. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cayrol, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Paulazo, Maria Alejandra. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinel Lamas, Diego J. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotópos; Argentina
Fil: Colombo, Lucas Luis. Universidad de Buenos Aires. Instituto de Oncología Angel H. Roffo. Area de Investigación; Argentina
Fil: Colombo, Lucas Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotópos; Argentina
Fil: Cremaschi, Graciela A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cremaschi, Graciela A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotópos; Argentina
Fil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Barreiro Arcos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barreiro Arcos, María Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Abstract: We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development have been studied, but the results are still controversial. Herein, we show the modulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistent with the rate of cell division determined by staining tumor cells with carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly from the euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increased in hypothyroid mice. Intratumoral and peritumoral vasculogenesis was increased only in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis.
description Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/8787
0022-0795 (impreso)
1479-6805 (online)
10.1530/JOE-14-0159
24928937
Sterle HA, Valli E, Cayrol F, et al. Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis [en línea]. Journal of Endocrinology. 2014;222(2):243-255. doi:10.1530/JOE-14-0159 Registro en: https://repositorio.uca.edu.ar/handle/123456789/8787
url https://repositorio.uca.edu.ar/handle/123456789/8787
identifier_str_mv 0022-0795 (impreso)
1479-6805 (online)
10.1530/JOE-14-0159
24928937
Sterle HA, Valli E, Cayrol F, et al. Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis [en línea]. Journal of Endocrinology. 2014;222(2):243-255. doi:10.1530/JOE-14-0159 Registro en: https://repositorio.uca.edu.ar/handle/123456789/8787
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv Journal of Endocrinology. 2014;222(2):243-255
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.13397

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