Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis

Autores
Sterle, Helena Andrea; Valli, Eduardo; Cayrol, Maria Florencia; Paulazo, Maria Alejandra; Martinel Lamas, Diego José; Díaz Flaqué, María Celeste; Klecha, Alicia Juana; Colombo, L.; Medina, Vanina Araceli; Cremaschi, Graciela Alicia; Barreiro Arcos, María Laura
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development have been studied, but the results are still controversial. Herein, we show the modulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistent with the rate of cell division determined by staining tumor cells with carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly from the euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increased in hypothyroid mice. Intratumoral and peritumoral vasculogenesis was increased only in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis.
Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina ; Argentina
Fil: Valli, Eduardo. Pontificia Universidad Católica Argentina ; Argentina
Fil: Cayrol, Maria Florencia. Pontificia Universidad Católica Argentina ; Argentina
Fil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Martinel Lamas, Diego José. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz Flaqué, María Celeste. Pontificia Universidad Católica Argentina ; Argentina
Fil: Klecha, Alicia Juana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Colombo, L.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología ; Argentina
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Pontificia Universidad Católica Argentina ; Argentina
Fil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Materia
THYROI HORMONES
T LYMPHOMA
CELL CYCLE
ANGIOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/35988
Acceder
id CONICETDig_22aaa084009cd0e2df48b54be6227b2e
oai_identifier_str oai:ri.conicet.gov.ar:11336/35988
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesisSterle, Helena AndreaValli, EduardoCayrol, Maria FlorenciaPaulazo, Maria AlejandraMartinel Lamas, Diego JoséDíaz Flaqué, María CelesteKlecha, Alicia JuanaColombo, L.Medina, Vanina AraceliCremaschi, Graciela AliciaBarreiro Arcos, María LauraTHYROI HORMONEST LYMPHOMACELL CYCLEANGIOGENESIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development have been studied, but the results are still controversial. Herein, we show the modulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistent with the rate of cell division determined by staining tumor cells with carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly from the euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increased in hypothyroid mice. Intratumoral and peritumoral vasculogenesis was increased only in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis.Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina ; ArgentinaFil: Valli, Eduardo. Pontificia Universidad Católica Argentina ; ArgentinaFil: Cayrol, Maria Florencia. Pontificia Universidad Católica Argentina ; ArgentinaFil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Martinel Lamas, Diego José. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Díaz Flaqué, María Celeste. Pontificia Universidad Católica Argentina ; ArgentinaFil: Klecha, Alicia Juana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Colombo, L.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología ; ArgentinaFil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cremaschi, Graciela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Pontificia Universidad Católica Argentina ; ArgentinaFil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaBioScientifica2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35988Sterle, Helena Andrea; Valli, Eduardo; Cayrol, Maria Florencia; Paulazo, Maria Alejandra; Martinel Lamas, Diego José; et al.; Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis; BioScientifica; Journal of Endocrinology; 222; 8-2014; 243-2550022-0795CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1530/JOE-14-0159info:eu-repo/semantics/altIdentifier/url/http://joe.endocrinology-journals.org/content/222/2/243info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:43Zoai:ri.conicet.gov.ar:11336/35988instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:43.383CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
spellingShingle Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
Sterle, Helena Andrea
THYROI HORMONES
T LYMPHOMA
CELL CYCLE
ANGIOGENESIS
title_short Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_full Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_fullStr Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_full_unstemmed Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
title_sort Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis
dc.creator.none.fl_str_mv Sterle, Helena Andrea
Valli, Eduardo
Cayrol, Maria Florencia
Paulazo, Maria Alejandra
Martinel Lamas, Diego José
Díaz Flaqué, María Celeste
Klecha, Alicia Juana
Colombo, L.
Medina, Vanina Araceli
Cremaschi, Graciela Alicia
Barreiro Arcos, María Laura
author Sterle, Helena Andrea
author_facet Sterle, Helena Andrea
Valli, Eduardo
Cayrol, Maria Florencia
Paulazo, Maria Alejandra
Martinel Lamas, Diego José
Díaz Flaqué, María Celeste
Klecha, Alicia Juana
Colombo, L.
Medina, Vanina Araceli
Cremaschi, Graciela Alicia
Barreiro Arcos, María Laura
author_role author
author2 Valli, Eduardo
Cayrol, Maria Florencia
Paulazo, Maria Alejandra
Martinel Lamas, Diego José
Díaz Flaqué, María Celeste
Klecha, Alicia Juana
Colombo, L.
Medina, Vanina Araceli
Cremaschi, Graciela Alicia
Barreiro Arcos, María Laura
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv THYROI HORMONES
T LYMPHOMA
CELL CYCLE
ANGIOGENESIS
topic THYROI HORMONES
T LYMPHOMA
CELL CYCLE
ANGIOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development have been studied, but the results are still controversial. Herein, we show the modulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistent with the rate of cell division determined by staining tumor cells with carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly from the euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increased in hypothyroid mice. Intratumoral and peritumoral vasculogenesis was increased only in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis.
Fil: Sterle, Helena Andrea. Pontificia Universidad Católica Argentina ; Argentina
Fil: Valli, Eduardo. Pontificia Universidad Católica Argentina ; Argentina
Fil: Cayrol, Maria Florencia. Pontificia Universidad Católica Argentina ; Argentina
Fil: Paulazo, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Martinel Lamas, Diego José. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz Flaqué, María Celeste. Pontificia Universidad Católica Argentina ; Argentina
Fil: Klecha, Alicia Juana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Colombo, L.. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología ; Argentina
Fil: Medina, Vanina Araceli. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Pontificia Universidad Católica Argentina ; Argentina
Fil: Barreiro Arcos, María Laura. Pontificia Universidad Católica Argentina ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
description We have shown in vitro that thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T lymphoma cells. The effects of THs on tumor development have been studied, but the results are still controversial. Herein, we show the modulatory action of thyroid status on the in vivo growth of T lymphoma cells. For this purpose, euthyroid, hypothyroid, and hyperthyroid mice received inoculations of EL4 cells to allow the development of solid tumors. Tumors in the hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid tumors and the increased tumor volume. These results are consistent with the rate of cell division determined by staining tumor cells with carboxyfluorescein succinimidyl ester. Additionally, hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly from the euthyroid controls with respect to these parameters. Additionally, only tumors from hyperthyroid animals had increased expression levels of proliferating cell nuclear antigen and active caspase 3. Differential expression of cell cycle regulatory proteins was also observed. The levels of cyclins D1 and D3 were augmented in the tumors of the hyperthyroid animals, whereas the cell cycle inhibitors p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the tumor suppressor p53 (TRP53) were increased in hypothyroid mice. Intratumoral and peritumoral vasculogenesis was increased only in hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T lymphoma through the regulation of cyclin, cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/35988
Sterle, Helena Andrea; Valli, Eduardo; Cayrol, Maria Florencia; Paulazo, Maria Alejandra; Martinel Lamas, Diego José; et al.; Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis; BioScientifica; Journal of Endocrinology; 222; 8-2014; 243-255
0022-0795
CONICET Digital
CONICET
url http://hdl.handle.net/11336/35988
identifier_str_mv Sterle, Helena Andrea; Valli, Eduardo; Cayrol, Maria Florencia; Paulazo, Maria Alejandra; Martinel Lamas, Diego José; et al.; Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis; BioScientifica; Journal of Endocrinology; 222; 8-2014; 243-255
0022-0795
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1530/JOE-14-0159
info:eu-repo/semantics/altIdentifier/url/http://joe.endocrinology-journals.org/content/222/2/243
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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