Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subs...

Autores
Jiménez Ortega, Vanesa; Fernández Mateos, María P.; Cano Barquilla, Pilar; Cardinali, Daniel Pedro; Esquifino, Ana I.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Fernández Mateos, María P. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celular; España
Fil: Fernández Mateos, María P. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular; España
Fil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular; España
Fil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina
Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Abstract: Excessive alcohol consumption continues to be a major public health problem, particularly in the adolescent and young adult populations. Generally, such a behavior tends to be confined to the weekends, to attain frequently binge drinking. This study in peripubertal male rats compares the effect of the discontinuous feeding of a liquid diet containing a moderate amount of ethanol (6.2 % wt/vol) to that of continuous ethanol administration or a control diet, taking as end points the 24‐h variations of plasma prolactin levels and mitogenic responses and lymphocyte subset populations in submaxillary lymph nodes and spleen. Animals received the ethanol liquid diet starting on day 35 of life, the diet being similar to that given to controls except for that maltose was isocalorically replaced by ethanol. Ethanol provided 36% of the total caloric content. Every week, the discontinuous ethanol group received the ethanol diet for 3 days and the control liquid diet for the remaining 4 days. After 4 weeks, rats were killed at 6 time intervals, beginning at 0900 h. A significant decrease of splenic cells´response to concanavalin A, and of lymph node and splenic cells’ response to lipopolysaccharide was found in rats under the discontinuous ethanol regime, as compared to control or ethanolchronic rats. Under discontinuous ethanol feeding mean values of lymph node and splenic CD8+ and CD4+‐CD8+cells decreased, whereas those of lymph node and splenic T cells, and splenic B cells, augmented. In rats chronically fed with ethanol, splenic mean levels of CD8+ and CD4+‐CD8+cells augmented. Both modalities of ethanol administration disrupted the 24 h variation in immune function seen in controls. Mean plasma prolactin levels 3 increased by 3.6 and 8.5 fold in rats chronically or discontinuously fed with alcohol, respectively. The immune parameters examined in an additional group of rats fed regular chow and water ad libitum did not differ significantly from control liquid diet. The results support the view that the discontinuous drinking of a moderate amount of ethanol can be more harmful for the immune system than a continuous ethanol intake, presumably by inducing a greater stress as indicated by the augmented plasma prolactin levels observed.
Fuente
Alcohol. 2011, 45 (2)
Materia
GANGLIOS LINFATICOS
SUBGRUPOS LINFOCITARIOS
RITMO CIRCADIANO
BAZO
STRESS
CONSUMO DE BEBIDAS ALCOHOLICAS
PROLACTINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/1631

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oai_identifier_str oai:ucacris:123456789/1631
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populationsJiménez Ortega, VanesaFernández Mateos, María P.Cano Barquilla, PilarCardinali, Daniel PedroEsquifino, Ana I.GANGLIOS LINFATICOSSUBGRUPOS LINFOCITARIOSRITMO CIRCADIANOBAZOSTRESSCONSUMO DE BEBIDAS ALCOHOLICASPROLACTINAFil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Fernández Mateos, María P. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celular; EspañaFil: Fernández Mateos, María P. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular; EspañaFil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular; EspañaFil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; ArgentinaFil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaAbstract: Excessive alcohol consumption continues to be a major public health problem, particularly in the adolescent and young adult populations. Generally, such a behavior tends to be confined to the weekends, to attain frequently binge drinking. This study in peripubertal male rats compares the effect of the discontinuous feeding of a liquid diet containing a moderate amount of ethanol (6.2 % wt/vol) to that of continuous ethanol administration or a control diet, taking as end points the 24‐h variations of plasma prolactin levels and mitogenic responses and lymphocyte subset populations in submaxillary lymph nodes and spleen. Animals received the ethanol liquid diet starting on day 35 of life, the diet being similar to that given to controls except for that maltose was isocalorically replaced by ethanol. Ethanol provided 36% of the total caloric content. Every week, the discontinuous ethanol group received the ethanol diet for 3 days and the control liquid diet for the remaining 4 days. After 4 weeks, rats were killed at 6 time intervals, beginning at 0900 h. A significant decrease of splenic cells´response to concanavalin A, and of lymph node and splenic cells’ response to lipopolysaccharide was found in rats under the discontinuous ethanol regime, as compared to control or ethanolchronic rats. Under discontinuous ethanol feeding mean values of lymph node and splenic CD8+ and CD4+‐CD8+cells decreased, whereas those of lymph node and splenic T cells, and splenic B cells, augmented. In rats chronically fed with ethanol, splenic mean levels of CD8+ and CD4+‐CD8+cells augmented. Both modalities of ethanol administration disrupted the 24 h variation in immune function seen in controls. Mean plasma prolactin levels 3 increased by 3.6 and 8.5 fold in rats chronically or discontinuously fed with alcohol, respectively. The immune parameters examined in an additional group of rats fed regular chow and water ad libitum did not differ significantly from control liquid diet. The results support the view that the discontinuous drinking of a moderate amount of ethanol can be more harmful for the immune system than a continuous ethanol intake, presumably by inducing a greater stress as indicated by the augmented plasma prolactin levels observed.Elsevier2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/16310741-832910.1016/j.alcohol.2010.08.008Jiménez-Ortega, V., et al. Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations [en línea]. Preprint de artículo publicado en Alcohol. 2011, 45 (2). doi:10.1016/j.alcohol.2010.08.008. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1631Alcohol. 2011, 45 (2)reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaengenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:55:19Zoai:ucacris:123456789/1631instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:55:20.021Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
title Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
spellingShingle Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
Jiménez Ortega, Vanesa
GANGLIOS LINFATICOS
SUBGRUPOS LINFOCITARIOS
RITMO CIRCADIANO
BAZO
STRESS
CONSUMO DE BEBIDAS ALCOHOLICAS
PROLACTINA
title_short Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
title_full Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
title_fullStr Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
title_full_unstemmed Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
title_sort Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
dc.creator.none.fl_str_mv Jiménez Ortega, Vanesa
Fernández Mateos, María P.
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author Jiménez Ortega, Vanesa
author_facet Jiménez Ortega, Vanesa
Fernández Mateos, María P.
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author_role author
author2 Fernández Mateos, María P.
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author2_role author
author
author
author
dc.subject.none.fl_str_mv GANGLIOS LINFATICOS
SUBGRUPOS LINFOCITARIOS
RITMO CIRCADIANO
BAZO
STRESS
CONSUMO DE BEBIDAS ALCOHOLICAS
PROLACTINA
topic GANGLIOS LINFATICOS
SUBGRUPOS LINFOCITARIOS
RITMO CIRCADIANO
BAZO
STRESS
CONSUMO DE BEBIDAS ALCOHOLICAS
PROLACTINA
dc.description.none.fl_txt_mv Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Fernández Mateos, María P. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celular; España
Fil: Fernández Mateos, María P. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular; España
Fil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular; España
Fil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina
Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Abstract: Excessive alcohol consumption continues to be a major public health problem, particularly in the adolescent and young adult populations. Generally, such a behavior tends to be confined to the weekends, to attain frequently binge drinking. This study in peripubertal male rats compares the effect of the discontinuous feeding of a liquid diet containing a moderate amount of ethanol (6.2 % wt/vol) to that of continuous ethanol administration or a control diet, taking as end points the 24‐h variations of plasma prolactin levels and mitogenic responses and lymphocyte subset populations in submaxillary lymph nodes and spleen. Animals received the ethanol liquid diet starting on day 35 of life, the diet being similar to that given to controls except for that maltose was isocalorically replaced by ethanol. Ethanol provided 36% of the total caloric content. Every week, the discontinuous ethanol group received the ethanol diet for 3 days and the control liquid diet for the remaining 4 days. After 4 weeks, rats were killed at 6 time intervals, beginning at 0900 h. A significant decrease of splenic cells´response to concanavalin A, and of lymph node and splenic cells’ response to lipopolysaccharide was found in rats under the discontinuous ethanol regime, as compared to control or ethanolchronic rats. Under discontinuous ethanol feeding mean values of lymph node and splenic CD8+ and CD4+‐CD8+cells decreased, whereas those of lymph node and splenic T cells, and splenic B cells, augmented. In rats chronically fed with ethanol, splenic mean levels of CD8+ and CD4+‐CD8+cells augmented. Both modalities of ethanol administration disrupted the 24 h variation in immune function seen in controls. Mean plasma prolactin levels 3 increased by 3.6 and 8.5 fold in rats chronically or discontinuously fed with alcohol, respectively. The immune parameters examined in an additional group of rats fed regular chow and water ad libitum did not differ significantly from control liquid diet. The results support the view that the discontinuous drinking of a moderate amount of ethanol can be more harmful for the immune system than a continuous ethanol intake, presumably by inducing a greater stress as indicated by the augmented plasma prolactin levels observed.
description Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/1631
0741-8329
10.1016/j.alcohol.2010.08.008
Jiménez-Ortega, V., et al. Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations [en línea]. Preprint de artículo publicado en Alcohol. 2011, 45 (2). doi:10.1016/j.alcohol.2010.08.008. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1631
url https://repositorio.uca.edu.ar/handle/123456789/1631
identifier_str_mv 0741-8329
10.1016/j.alcohol.2010.08.008
Jiménez-Ortega, V., et al. Continuous vs. discontinuous drinking of an ethanol liquid diet in peripubertal rats : effect on 24-hour variation of lymph node and splenic mitogenic responses and lymphocyte subset populations [en línea]. Preprint de artículo publicado en Alcohol. 2011, 45 (2). doi:10.1016/j.alcohol.2010.08.008. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1631
dc.language.none.fl_str_mv eng
eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Alcohol. 2011, 45 (2)
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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