Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subse...

Autores
Jiménez Ortega, Vanesa; Fernández Mateos, María P.; Barquilla, Pilar Cano; Cardinali, Daniel Pedro; Esquifino, Ana I.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Excessive alcohol consumption continues to be a major public health problem, particularly in the adolescent and young adult populations. Generally, such a behavior tends to be confined to the weekends, to attain frequently binge drinking. This study in peripubertal male rats compares the effect of the discontinuous feeding of a liquid diet containing a moderate amount of ethanol (6.2% wt/vol) to that of continuous ethanol administration or a control diet, taking as end points the 24-h variations of plasma prolactin levels and mitogenic responses and lymphocyte subset populations in submaxillary lymph nodes and spleen. Animals received the ethanol liquid diet starting on day 35 of life, the diet being similar to that given to controls except for that maltose was isocalorically replaced by ethanol. Ethanol provided 36% of the total caloric content. Every week, the discontinuous ethanol group received the ethanol diet for 3 days and the control liquid diet for the remaining 4 days. After 4 weeks, rats were killed at six time intervals, beginning at 0900h. A significant decrease of splenic cells' response to concanavalin A, and of lymph node and splenic cells' response to lipopolysaccharide was found in rats under the discontinuous ethanol regime, when compared with control- or ethanol-chronic rats. Under discontinuous ethanol feeding, mean values of lymph node and splenic CD8+ and CD4+-CD8+cells decreased, whereas those of lymph node and splenic T cells, and splenic B cells, augmented. In rats chronically fed with ethanol, splenic mean levels of CD8+ and CD4+-CD8+cells augmented. Both modalities of ethanol administration disrupted the 24h variation in immune function seen in controls. Mean plasma prolactin levels increased by 3.6-fold and 8.5-fold in rats chronically or discontinuously fed with alcohol, respectively. The immune parameters examined in an additional group of rats fed regular chow and water ad libitum did not differ significantly from control liquid diet. The results support the view that the discontinuous drinking of a moderate amount of ethanol can be more harmful for the immune system than a continuous ethanol intake, presumably by inducing a greater stress as indicated by the augmented plasma prolactin levels observed.
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; España
Fil: Fernández Mateos, María P.. Universidad Complutense de Madrid; España
Fil: Barquilla, Pilar Cano. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
Materia
BINGE DRINKING
MITOGENIC RESPONSES
LYMPHOCYTE SUBSETS
CIRCADIAN RHYTHMS
PROLACTIN
STRESS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192061
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/192061
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populationsJiménez Ortega, VanesaFernández Mateos, María P.Barquilla, Pilar CanoCardinali, Daniel PedroEsquifino, Ana I.BINGE DRINKINGMITOGENIC RESPONSESLYMPHOCYTE SUBSETSCIRCADIAN RHYTHMSPROLACTINSTRESShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Excessive alcohol consumption continues to be a major public health problem, particularly in the adolescent and young adult populations. Generally, such a behavior tends to be confined to the weekends, to attain frequently binge drinking. This study in peripubertal male rats compares the effect of the discontinuous feeding of a liquid diet containing a moderate amount of ethanol (6.2% wt/vol) to that of continuous ethanol administration or a control diet, taking as end points the 24-h variations of plasma prolactin levels and mitogenic responses and lymphocyte subset populations in submaxillary lymph nodes and spleen. Animals received the ethanol liquid diet starting on day 35 of life, the diet being similar to that given to controls except for that maltose was isocalorically replaced by ethanol. Ethanol provided 36% of the total caloric content. Every week, the discontinuous ethanol group received the ethanol diet for 3 days and the control liquid diet for the remaining 4 days. After 4 weeks, rats were killed at six time intervals, beginning at 0900h. A significant decrease of splenic cells' response to concanavalin A, and of lymph node and splenic cells' response to lipopolysaccharide was found in rats under the discontinuous ethanol regime, when compared with control- or ethanol-chronic rats. Under discontinuous ethanol feeding, mean values of lymph node and splenic CD8+ and CD4+-CD8+cells decreased, whereas those of lymph node and splenic T cells, and splenic B cells, augmented. In rats chronically fed with ethanol, splenic mean levels of CD8+ and CD4+-CD8+cells augmented. Both modalities of ethanol administration disrupted the 24h variation in immune function seen in controls. Mean plasma prolactin levels increased by 3.6-fold and 8.5-fold in rats chronically or discontinuously fed with alcohol, respectively. The immune parameters examined in an additional group of rats fed regular chow and water ad libitum did not differ significantly from control liquid diet. The results support the view that the discontinuous drinking of a moderate amount of ethanol can be more harmful for the immune system than a continuous ethanol intake, presumably by inducing a greater stress as indicated by the augmented plasma prolactin levels observed.Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; EspañaFil: Fernández Mateos, María P.. Universidad Complutense de Madrid; EspañaFil: Barquilla, Pilar Cano. Universidad Complutense de Madrid; EspañaFil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Esquifino, Ana I.. Universidad Complutense de Madrid; EspañaElsevier Science Inc.2011-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192061Jiménez Ortega, Vanesa; Fernández Mateos, María P.; Barquilla, Pilar Cano; Cardinali, Daniel Pedro; Esquifino, Ana I.; Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations; Elsevier Science Inc.; Alcohol; 45; 2; 3-2011; 183-1920741-8329CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0741832910001175info:eu-repo/semantics/altIdentifier/doi/10.1016/j.alcohol.2010.08.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:45:15Zoai:ri.conicet.gov.ar:11336/192061instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:45:15.597CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
title Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
spellingShingle Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
Jiménez Ortega, Vanesa
BINGE DRINKING
MITOGENIC RESPONSES
LYMPHOCYTE SUBSETS
CIRCADIAN RHYTHMS
PROLACTIN
STRESS
title_short Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
title_full Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
title_fullStr Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
title_full_unstemmed Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
title_sort Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations
dc.creator.none.fl_str_mv Jiménez Ortega, Vanesa
Fernández Mateos, María P.
Barquilla, Pilar Cano
Cardinali, Daniel Pedro
Esquifino, Ana I.
author Jiménez Ortega, Vanesa
author_facet Jiménez Ortega, Vanesa
Fernández Mateos, María P.
Barquilla, Pilar Cano
Cardinali, Daniel Pedro
Esquifino, Ana I.
author_role author
author2 Fernández Mateos, María P.
Barquilla, Pilar Cano
Cardinali, Daniel Pedro
Esquifino, Ana I.
author2_role author
author
author
author
dc.subject.none.fl_str_mv BINGE DRINKING
MITOGENIC RESPONSES
LYMPHOCYTE SUBSETS
CIRCADIAN RHYTHMS
PROLACTIN
STRESS
topic BINGE DRINKING
MITOGENIC RESPONSES
LYMPHOCYTE SUBSETS
CIRCADIAN RHYTHMS
PROLACTIN
STRESS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Excessive alcohol consumption continues to be a major public health problem, particularly in the adolescent and young adult populations. Generally, such a behavior tends to be confined to the weekends, to attain frequently binge drinking. This study in peripubertal male rats compares the effect of the discontinuous feeding of a liquid diet containing a moderate amount of ethanol (6.2% wt/vol) to that of continuous ethanol administration or a control diet, taking as end points the 24-h variations of plasma prolactin levels and mitogenic responses and lymphocyte subset populations in submaxillary lymph nodes and spleen. Animals received the ethanol liquid diet starting on day 35 of life, the diet being similar to that given to controls except for that maltose was isocalorically replaced by ethanol. Ethanol provided 36% of the total caloric content. Every week, the discontinuous ethanol group received the ethanol diet for 3 days and the control liquid diet for the remaining 4 days. After 4 weeks, rats were killed at six time intervals, beginning at 0900h. A significant decrease of splenic cells' response to concanavalin A, and of lymph node and splenic cells' response to lipopolysaccharide was found in rats under the discontinuous ethanol regime, when compared with control- or ethanol-chronic rats. Under discontinuous ethanol feeding, mean values of lymph node and splenic CD8+ and CD4+-CD8+cells decreased, whereas those of lymph node and splenic T cells, and splenic B cells, augmented. In rats chronically fed with ethanol, splenic mean levels of CD8+ and CD4+-CD8+cells augmented. Both modalities of ethanol administration disrupted the 24h variation in immune function seen in controls. Mean plasma prolactin levels increased by 3.6-fold and 8.5-fold in rats chronically or discontinuously fed with alcohol, respectively. The immune parameters examined in an additional group of rats fed regular chow and water ad libitum did not differ significantly from control liquid diet. The results support the view that the discontinuous drinking of a moderate amount of ethanol can be more harmful for the immune system than a continuous ethanol intake, presumably by inducing a greater stress as indicated by the augmented plasma prolactin levels observed.
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; España
Fil: Fernández Mateos, María P.. Universidad Complutense de Madrid; España
Fil: Barquilla, Pilar Cano. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
description Excessive alcohol consumption continues to be a major public health problem, particularly in the adolescent and young adult populations. Generally, such a behavior tends to be confined to the weekends, to attain frequently binge drinking. This study in peripubertal male rats compares the effect of the discontinuous feeding of a liquid diet containing a moderate amount of ethanol (6.2% wt/vol) to that of continuous ethanol administration or a control diet, taking as end points the 24-h variations of plasma prolactin levels and mitogenic responses and lymphocyte subset populations in submaxillary lymph nodes and spleen. Animals received the ethanol liquid diet starting on day 35 of life, the diet being similar to that given to controls except for that maltose was isocalorically replaced by ethanol. Ethanol provided 36% of the total caloric content. Every week, the discontinuous ethanol group received the ethanol diet for 3 days and the control liquid diet for the remaining 4 days. After 4 weeks, rats were killed at six time intervals, beginning at 0900h. A significant decrease of splenic cells' response to concanavalin A, and of lymph node and splenic cells' response to lipopolysaccharide was found in rats under the discontinuous ethanol regime, when compared with control- or ethanol-chronic rats. Under discontinuous ethanol feeding, mean values of lymph node and splenic CD8+ and CD4+-CD8+cells decreased, whereas those of lymph node and splenic T cells, and splenic B cells, augmented. In rats chronically fed with ethanol, splenic mean levels of CD8+ and CD4+-CD8+cells augmented. Both modalities of ethanol administration disrupted the 24h variation in immune function seen in controls. Mean plasma prolactin levels increased by 3.6-fold and 8.5-fold in rats chronically or discontinuously fed with alcohol, respectively. The immune parameters examined in an additional group of rats fed regular chow and water ad libitum did not differ significantly from control liquid diet. The results support the view that the discontinuous drinking of a moderate amount of ethanol can be more harmful for the immune system than a continuous ethanol intake, presumably by inducing a greater stress as indicated by the augmented plasma prolactin levels observed.
publishDate 2011
dc.date.none.fl_str_mv 2011-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192061
Jiménez Ortega, Vanesa; Fernández Mateos, María P.; Barquilla, Pilar Cano; Cardinali, Daniel Pedro; Esquifino, Ana I.; Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations; Elsevier Science Inc.; Alcohol; 45; 2; 3-2011; 183-192
0741-8329
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192061
identifier_str_mv Jiménez Ortega, Vanesa; Fernández Mateos, María P.; Barquilla, Pilar Cano; Cardinali, Daniel Pedro; Esquifino, Ana I.; Continuous versus discontinuous drinking of an ethanol liquid diet in peripubertal rats: Effect on 24-h variation of lymph node and splenic mitogenic responses and lymphocyte subset populations; Elsevier Science Inc.; Alcohol; 45; 2; 3-2011; 183-192
0741-8329
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0741832910001175
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.alcohol.2010.08.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc.
publisher.none.fl_str_mv Elsevier Science Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.000565

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