Structural biology of coronavirus ion channels
- Autores
- Barrantes, Francisco José
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
Fil: Barrantes, Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: Viral infection compromises specific organelles of the cell and readdresses its functional resources to satisfy the needs of the invading body. Around 70% of the coronavirus positive-sense single-stranded RNA encodes proteins involved in replication, and these viruses essentially take over the biosynthetic and transport mechanisms to ensure the efficient replication of their genome and trafficking of their virions. Some coronaviruses encode genes for ion-channel proteins – the envelope protein E (orf4a), orf3a and orf8 – which they successfully employ to take control of the endoplasmic reticulum–Golgi complex intermediate compartment or ERGIC. The E protein, which is one of the four structural proteins of SARS-CoV-2 and other coronaviruses, assembles its transmembrane protomers into homopentameric channels with mild cationic selectivity. Orf3a forms homodimers and homotetramers. Both carry a PDZ-binding domain, lending them the versatility to interact with more than 400 target proteins in infected host cells. Orf8 is a very short 29-amino-acid single-passage transmembrane peptide that forms cation-selective channels when assembled in lipid bilayers. This review addresses the contribution of biophysical and structural biology approaches that unravel different facets of coronavirus ion channels, their effects on the cellular machinery of infected cells and some structure–functional correlations with ion channels of higher organisms. - Fuente
- Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4)
- Materia
-
MEDICINA
BIOLOGIA
CORONAVIRUS
COVID-19
SARS-CoV-2
CANALES IONICOS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Pontificia Universidad Católica Argentina
- OAI Identificador
- oai:ucacris:123456789/11364
Ver los metadatos del registro completo
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Structural biology of coronavirus ion channelsBarrantes, Francisco JoséMEDICINABIOLOGIACORONAVIRUSCOVID-19SARS-CoV-2CANALES IONICOSFil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; ArgentinaFil: Barrantes, Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAbstract: Viral infection compromises specific organelles of the cell and readdresses its functional resources to satisfy the needs of the invading body. Around 70% of the coronavirus positive-sense single-stranded RNA encodes proteins involved in replication, and these viruses essentially take over the biosynthetic and transport mechanisms to ensure the efficient replication of their genome and trafficking of their virions. Some coronaviruses encode genes for ion-channel proteins – the envelope protein E (orf4a), orf3a and orf8 – which they successfully employ to take control of the endoplasmic reticulum–Golgi complex intermediate compartment or ERGIC. The E protein, which is one of the four structural proteins of SARS-CoV-2 and other coronaviruses, assembles its transmembrane protomers into homopentameric channels with mild cationic selectivity. Orf3a forms homodimers and homotetramers. Both carry a PDZ-binding domain, lending them the versatility to interact with more than 400 target proteins in infected host cells. Orf8 is a very short 29-amino-acid single-passage transmembrane peptide that forms cation-selective channels when assembled in lipid bilayers. This review addresses the contribution of biophysical and structural biology approaches that unravel different facets of coronavirus ion channels, their effects on the cellular machinery of infected cells and some structure–functional correlations with ion channels of higher organisms.C. S. Bond, University of Western Australia, Crawley, Australia2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/113642059-7983https://doi.org/10.1107/S205979832100143133825700Barrantes F. J. Structural biology of coronavirus ion channels [en línea]. Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4) Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11364Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4)reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:57:43Zoai:ucacris:123456789/11364instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:57:43.515Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse |
dc.title.none.fl_str_mv |
Structural biology of coronavirus ion channels |
title |
Structural biology of coronavirus ion channels |
spellingShingle |
Structural biology of coronavirus ion channels Barrantes, Francisco José MEDICINA BIOLOGIA CORONAVIRUS COVID-19 SARS-CoV-2 CANALES IONICOS |
title_short |
Structural biology of coronavirus ion channels |
title_full |
Structural biology of coronavirus ion channels |
title_fullStr |
Structural biology of coronavirus ion channels |
title_full_unstemmed |
Structural biology of coronavirus ion channels |
title_sort |
Structural biology of coronavirus ion channels |
dc.creator.none.fl_str_mv |
Barrantes, Francisco José |
author |
Barrantes, Francisco José |
author_facet |
Barrantes, Francisco José |
author_role |
author |
dc.subject.none.fl_str_mv |
MEDICINA BIOLOGIA CORONAVIRUS COVID-19 SARS-CoV-2 CANALES IONICOS |
topic |
MEDICINA BIOLOGIA CORONAVIRUS COVID-19 SARS-CoV-2 CANALES IONICOS |
dc.description.none.fl_txt_mv |
Fil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina Fil: Barrantes, Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Abstract: Viral infection compromises specific organelles of the cell and readdresses its functional resources to satisfy the needs of the invading body. Around 70% of the coronavirus positive-sense single-stranded RNA encodes proteins involved in replication, and these viruses essentially take over the biosynthetic and transport mechanisms to ensure the efficient replication of their genome and trafficking of their virions. Some coronaviruses encode genes for ion-channel proteins – the envelope protein E (orf4a), orf3a and orf8 – which they successfully employ to take control of the endoplasmic reticulum–Golgi complex intermediate compartment or ERGIC. The E protein, which is one of the four structural proteins of SARS-CoV-2 and other coronaviruses, assembles its transmembrane protomers into homopentameric channels with mild cationic selectivity. Orf3a forms homodimers and homotetramers. Both carry a PDZ-binding domain, lending them the versatility to interact with more than 400 target proteins in infected host cells. Orf8 is a very short 29-amino-acid single-passage transmembrane peptide that forms cation-selective channels when assembled in lipid bilayers. This review addresses the contribution of biophysical and structural biology approaches that unravel different facets of coronavirus ion channels, their effects on the cellular machinery of infected cells and some structure–functional correlations with ion channels of higher organisms. |
description |
Fil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://repositorio.uca.edu.ar/handle/123456789/11364 2059-7983 https://doi.org/10.1107/S2059798321001431 33825700 Barrantes F. J. Structural biology of coronavirus ion channels [en línea]. Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4) Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11364 |
url |
https://repositorio.uca.edu.ar/handle/123456789/11364 https://doi.org/10.1107/S2059798321001431 |
identifier_str_mv |
2059-7983 33825700 Barrantes F. J. Structural biology of coronavirus ion channels [en línea]. Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4) Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11364 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
C. S. Bond, University of Western Australia, Crawley, Australia |
publisher.none.fl_str_mv |
C. S. Bond, University of Western Australia, Crawley, Australia |
dc.source.none.fl_str_mv |
Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4) reponame:Repositorio Institucional (UCA) instname:Pontificia Universidad Católica Argentina |
reponame_str |
Repositorio Institucional (UCA) |
collection |
Repositorio Institucional (UCA) |
instname_str |
Pontificia Universidad Católica Argentina |
repository.name.fl_str_mv |
Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina |
repository.mail.fl_str_mv |
claudia_fernandez@uca.edu.ar |
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13.070432 |