Structural biology of coronavirus ion channels

Autores
Barrantes, Francisco José
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
Fil: Barrantes, Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: Viral infection compromises specific organelles of the cell and readdresses its functional resources to satisfy the needs of the invading body. Around 70% of the coronavirus positive-sense single-stranded RNA encodes proteins involved in replication, and these viruses essentially take over the biosynthetic and transport mechanisms to ensure the efficient replication of their genome and trafficking of their virions. Some coronaviruses encode genes for ion-channel proteins – the envelope protein E (orf4a), orf3a and orf8 – which they successfully employ to take control of the endoplasmic reticulum–Golgi complex intermediate compartment or ERGIC. The E protein, which is one of the four structural proteins of SARS-CoV-2 and other coronaviruses, assembles its transmembrane protomers into homopentameric channels with mild cationic selectivity. Orf3a forms homodimers and homotetramers. Both carry a PDZ-binding domain, lending them the versatility to interact with more than 400 target proteins in infected host cells. Orf8 is a very short 29-amino-acid single-passage transmembrane peptide that forms cation-selective channels when assembled in lipid bilayers. This review addresses the contribution of biophysical and structural biology approaches that unravel different facets of coronavirus ion channels, their effects on the cellular machinery of infected cells and some structure–functional correlations with ion channels of higher organisms.
Fuente
Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4)
Materia
MEDICINA
BIOLOGIA
CORONAVIRUS
COVID-19
SARS-CoV-2
CANALES IONICOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/11364

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oai_identifier_str oai:ucacris:123456789/11364
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Structural biology of coronavirus ion channelsBarrantes, Francisco JoséMEDICINABIOLOGIACORONAVIRUSCOVID-19SARS-CoV-2CANALES IONICOSFil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; ArgentinaFil: Barrantes, Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAbstract: Viral infection compromises specific organelles of the cell and readdresses its functional resources to satisfy the needs of the invading body. Around 70% of the coronavirus positive-sense single-stranded RNA encodes proteins involved in replication, and these viruses essentially take over the biosynthetic and transport mechanisms to ensure the efficient replication of their genome and trafficking of their virions. Some coronaviruses encode genes for ion-channel proteins – the envelope protein E (orf4a), orf3a and orf8 – which they successfully employ to take control of the endoplasmic reticulum–Golgi complex intermediate compartment or ERGIC. The E protein, which is one of the four structural proteins of SARS-CoV-2 and other coronaviruses, assembles its transmembrane protomers into homopentameric channels with mild cationic selectivity. Orf3a forms homodimers and homotetramers. Both carry a PDZ-binding domain, lending them the versatility to interact with more than 400 target proteins in infected host cells. Orf8 is a very short 29-amino-acid single-passage transmembrane peptide that forms cation-selective channels when assembled in lipid bilayers. This review addresses the contribution of biophysical and structural biology approaches that unravel different facets of coronavirus ion channels, their effects on the cellular machinery of infected cells and some structure–functional correlations with ion channels of higher organisms.C. S. Bond, University of Western Australia, Crawley, Australia2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/113642059-7983https://doi.org/10.1107/S205979832100143133825700Barrantes F. J. Structural biology of coronavirus ion channels [en línea]. Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4) Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11364Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4)reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:57:43Zoai:ucacris:123456789/11364instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:57:43.515Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Structural biology of coronavirus ion channels
title Structural biology of coronavirus ion channels
spellingShingle Structural biology of coronavirus ion channels
Barrantes, Francisco José
MEDICINA
BIOLOGIA
CORONAVIRUS
COVID-19
SARS-CoV-2
CANALES IONICOS
title_short Structural biology of coronavirus ion channels
title_full Structural biology of coronavirus ion channels
title_fullStr Structural biology of coronavirus ion channels
title_full_unstemmed Structural biology of coronavirus ion channels
title_sort Structural biology of coronavirus ion channels
dc.creator.none.fl_str_mv Barrantes, Francisco José
author Barrantes, Francisco José
author_facet Barrantes, Francisco José
author_role author
dc.subject.none.fl_str_mv MEDICINA
BIOLOGIA
CORONAVIRUS
COVID-19
SARS-CoV-2
CANALES IONICOS
topic MEDICINA
BIOLOGIA
CORONAVIRUS
COVID-19
SARS-CoV-2
CANALES IONICOS
dc.description.none.fl_txt_mv Fil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
Fil: Barrantes, Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: Viral infection compromises specific organelles of the cell and readdresses its functional resources to satisfy the needs of the invading body. Around 70% of the coronavirus positive-sense single-stranded RNA encodes proteins involved in replication, and these viruses essentially take over the biosynthetic and transport mechanisms to ensure the efficient replication of their genome and trafficking of their virions. Some coronaviruses encode genes for ion-channel proteins – the envelope protein E (orf4a), orf3a and orf8 – which they successfully employ to take control of the endoplasmic reticulum–Golgi complex intermediate compartment or ERGIC. The E protein, which is one of the four structural proteins of SARS-CoV-2 and other coronaviruses, assembles its transmembrane protomers into homopentameric channels with mild cationic selectivity. Orf3a forms homodimers and homotetramers. Both carry a PDZ-binding domain, lending them the versatility to interact with more than 400 target proteins in infected host cells. Orf8 is a very short 29-amino-acid single-passage transmembrane peptide that forms cation-selective channels when assembled in lipid bilayers. This review addresses the contribution of biophysical and structural biology approaches that unravel different facets of coronavirus ion channels, their effects on the cellular machinery of infected cells and some structure–functional correlations with ion channels of higher organisms.
description Fil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/11364
2059-7983
https://doi.org/10.1107/S2059798321001431
33825700
Barrantes F. J. Structural biology of coronavirus ion channels [en línea]. Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4) Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11364
url https://repositorio.uca.edu.ar/handle/123456789/11364
https://doi.org/10.1107/S2059798321001431
identifier_str_mv 2059-7983
33825700
Barrantes F. J. Structural biology of coronavirus ion channels [en línea]. Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4) Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11364
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv C. S. Bond, University of Western Australia, Crawley, Australia
publisher.none.fl_str_mv C. S. Bond, University of Western Australia, Crawley, Australia
dc.source.none.fl_str_mv Posprint del artículo publicado en Acta Crystallographica Section D Structural Biology. 2021, 77 (4)
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.070432