Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink

Autores
Molinari, J.; Florez, L.; Medrano, A.; Monsalve, L.; Ybarra, G.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations.
Fil: Molinari, J. Instituto Nacional de Tecnología Industrial. INTI-Procesos Superficiales; Argentina
Fil: Florez, L. Instituto Nacional de Tecnología Industrial. INTI-Procesos Superficiales; Argentina
Fil: Medrano, A. Instituto Nacional de Tecnología Industrial. INTI-Micro y Nanoelectrónica; Argentina
Fil: Monsalve, L. Instituto Nacional de Tecnología Industrial. INTI-Micro y Nanoelectrónica; Argentina
Fil: Ybarra, G. Instituto Nacional de Tecnología Industrial. INTI-Procesos Superficiales; Argentina
Fuente
Biosensors, 8(4)
Materia
Métodos electroquímicos
Sensores electroquímicos
Nanotubos de carbón
Alérgenos
Alimentos alergénicos
Poliestireno
Tintas
Biosensores
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-sa/4.0/
Repositorio
Repositorio Institucional del Instituto Nacional de Tecnología Industrial (INTI)
Institución
Instituto Nacional de Tecnología Industrial
OAI Identificador
nuevadc:Molinari2018Electrochemical_pdf
Acceder
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oai_identifier_str nuevadc:Molinari2018Electrochemical_pdf
network_acronym_str RIINTI
repository_id_str
network_name_str Repositorio Institucional del Instituto Nacional de Tecnología Industrial (INTI)
spelling Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube inkMolinari, J.Florez, L.Medrano, A.Monsalve, L.Ybarra, G.Métodos electroquímicosSensores electroquímicosNanotubos de carbónAlérgenosAlimentos alergénicosPoliestirenoTintasBiosensoresIn this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations.Fil: Molinari, J. Instituto Nacional de Tecnología Industrial. INTI-Procesos Superficiales; ArgentinaFil: Florez, L. Instituto Nacional de Tecnología Industrial. INTI-Procesos Superficiales; ArgentinaFil: Medrano, A. Instituto Nacional de Tecnología Industrial. INTI-Micro y Nanoelectrónica; ArgentinaFil: Monsalve, L. Instituto Nacional de Tecnología Industrial. INTI-Micro y Nanoelectrónica; ArgentinaFil: Ybarra, G. Instituto Nacional de Tecnología Industrial. INTI-Procesos Superficiales; ArgentinaMDPI2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfMolinari2018Electrochemical.pdfhttps://app.inti.gob.ar/greenstone3/sites/localsite/collect/nuevadc/index/assoc/Molinari/2018Elec.dir/doc.pdfBiosensors, 8(4)reponame:Repositorio Institucional del Instituto Nacional de Tecnología Industrial (INTI)instname:Instituto Nacional de Tecnología Industrialenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-sa/4.0/openAccess2025-09-04T11:43:05Znuevadc:Molinari2018Electrochemical_pdfinstacron:INTIInstitucionalhttps://app.inti.gob.ar/greenstone3/biblioOrganismo científico-tecnológicohttps://argentina.gob.ar/intihttps://app.inti.gob.ar/greenstone3/oaiserver?verb=Identifypfalcato@inti.gob.arArgentinaopendoar:2025-09-04 11:43:06.265Repositorio Institucional del Instituto Nacional de Tecnología Industrial (INTI) - Instituto Nacional de Tecnología Industrialfalse
dc.title.none.fl_str_mv Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
title Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
spellingShingle Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
Molinari, J.
Métodos electroquímicos
Sensores electroquímicos
Nanotubos de carbón
Alérgenos
Alimentos alergénicos
Poliestireno
Tintas
Biosensores
title_short Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
title_full Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
title_fullStr Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
title_full_unstemmed Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
title_sort Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
dc.creator.none.fl_str_mv Molinari, J.
Florez, L.
Medrano, A.
Monsalve, L.
Ybarra, G.
author Molinari, J.
author_facet Molinari, J.
Florez, L.
Medrano, A.
Monsalve, L.
Ybarra, G.
author_role author
author2 Florez, L.
Medrano, A.
Monsalve, L.
Ybarra, G.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Métodos electroquímicos
Sensores electroquímicos
Nanotubos de carbón
Alérgenos
Alimentos alergénicos
Poliestireno
Tintas
Biosensores
topic Métodos electroquímicos
Sensores electroquímicos
Nanotubos de carbón
Alérgenos
Alimentos alergénicos
Poliestireno
Tintas
Biosensores
dc.description.none.fl_txt_mv In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations.
Fil: Molinari, J. Instituto Nacional de Tecnología Industrial. INTI-Procesos Superficiales; Argentina
Fil: Florez, L. Instituto Nacional de Tecnología Industrial. INTI-Procesos Superficiales; Argentina
Fil: Medrano, A. Instituto Nacional de Tecnología Industrial. INTI-Micro y Nanoelectrónica; Argentina
Fil: Monsalve, L. Instituto Nacional de Tecnología Industrial. INTI-Micro y Nanoelectrónica; Argentina
Fil: Ybarra, G. Instituto Nacional de Tecnología Industrial. INTI-Procesos Superficiales; Argentina
description In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Molinari2018Electrochemical.pdf
https://app.inti.gob.ar/greenstone3/sites/localsite/collect/nuevadc/index/assoc/Molinari/2018Elec.dir/doc.pdf
identifier_str_mv Molinari2018Electrochemical.pdf
url https://app.inti.gob.ar/greenstone3/sites/localsite/collect/nuevadc/index/assoc/Molinari/2018Elec.dir/doc.pdf
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-sa/4.0/
openAccess
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-sa/4.0/
openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Biosensors, 8(4)
reponame:Repositorio Institucional del Instituto Nacional de Tecnología Industrial (INTI)
instname:Instituto Nacional de Tecnología Industrial
reponame_str Repositorio Institucional del Instituto Nacional de Tecnología Industrial (INTI)
collection Repositorio Institucional del Instituto Nacional de Tecnología Industrial (INTI)
instname_str Instituto Nacional de Tecnología Industrial
repository.name.fl_str_mv Repositorio Institucional del Instituto Nacional de Tecnología Industrial (INTI) - Instituto Nacional de Tecnología Industrial
repository.mail.fl_str_mv pfalcato@inti.gob.ar
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score 12.623145

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