Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink

Autores
Molinari, Judith; Florez, Laura Carolina; Medrano, Anahi Vanesa; Monsalve, Leandro Nicolas; Ybarra, Gabriel Omar
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations.
Fil: Molinari, Judith. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Florez, Laura Carolina. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Medrano, Anahi Vanesa. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Monsalve, Leandro Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Ybarra, Gabriel Omar. Instituto Nacional de Tecnología Industrial; Argentina
Materia
CARBON NANOTUBES
ELECTROCHEMICAL BIOSENSOR
FOOD ALLERGEN
Β-LACTOGLOBULIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/177753
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube inkMolinari, JudithFlorez, Laura CarolinaMedrano, Anahi VanesaMonsalve, Leandro NicolasYbarra, Gabriel OmarCARBON NANOTUBESELECTROCHEMICAL BIOSENSORFOOD ALLERGENΒ-LACTOGLOBULINhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations.Fil: Molinari, Judith. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Florez, Laura Carolina. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Medrano, Anahi Vanesa. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Monsalve, Leandro Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Ybarra, Gabriel Omar. Instituto Nacional de Tecnología Industrial; ArgentinaMDPI AG2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/177753Molinari, Judith; Florez, Laura Carolina; Medrano, Anahi Vanesa; Monsalve, Leandro Nicolas; Ybarra, Gabriel Omar; Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink; MDPI AG; Biosensors; 8; 4; 11-2018; 1-92079-6374CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6374/8/4/109info:eu-repo/semantics/altIdentifier/doi/10.3390/bios8040109info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:13Zoai:ri.conicet.gov.ar:11336/177753instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:13.763CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
title Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
spellingShingle Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
Molinari, Judith
CARBON NANOTUBES
ELECTROCHEMICAL BIOSENSOR
FOOD ALLERGEN
Β-LACTOGLOBULIN
title_short Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
title_full Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
title_fullStr Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
title_full_unstemmed Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
title_sort Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
dc.creator.none.fl_str_mv Molinari, Judith
Florez, Laura Carolina
Medrano, Anahi Vanesa
Monsalve, Leandro Nicolas
Ybarra, Gabriel Omar
author Molinari, Judith
author_facet Molinari, Judith
Florez, Laura Carolina
Medrano, Anahi Vanesa
Monsalve, Leandro Nicolas
Ybarra, Gabriel Omar
author_role author
author2 Florez, Laura Carolina
Medrano, Anahi Vanesa
Monsalve, Leandro Nicolas
Ybarra, Gabriel Omar
author2_role author
author
author
author
dc.subject.none.fl_str_mv CARBON NANOTUBES
ELECTROCHEMICAL BIOSENSOR
FOOD ALLERGEN
Β-LACTOGLOBULIN
topic CARBON NANOTUBES
ELECTROCHEMICAL BIOSENSOR
FOOD ALLERGEN
Β-LACTOGLOBULIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations.
Fil: Molinari, Judith. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Florez, Laura Carolina. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Medrano, Anahi Vanesa. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Monsalve, Leandro Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Ybarra, Gabriel Omar. Instituto Nacional de Tecnología Industrial; Argentina
description In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/177753
Molinari, Judith; Florez, Laura Carolina; Medrano, Anahi Vanesa; Monsalve, Leandro Nicolas; Ybarra, Gabriel Omar; Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink; MDPI AG; Biosensors; 8; 4; 11-2018; 1-9
2079-6374
CONICET Digital
CONICET
url http://hdl.handle.net/11336/177753
identifier_str_mv Molinari, Judith; Florez, Laura Carolina; Medrano, Anahi Vanesa; Monsalve, Leandro Nicolas; Ybarra, Gabriel Omar; Electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink; MDPI AG; Biosensors; 8; 4; 11-2018; 1-9
2079-6374
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6374/8/4/109
info:eu-repo/semantics/altIdentifier/doi/10.3390/bios8040109
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613690048905216
score 13.070432

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