CCQM K6.2 determination of total cholesterol in human serum

• Autores: Wise, Stephen A.; Phinney, Karen W.; Duewer, David L.; Sniegoski, Lorna T.; Welch, Michael J.; Pabello, Guiomar; Ávila Caldero, Marco A.; Qinde, Liu; Tong Kooi, Lee; Rego, Eliane; Garrido, Bruno; Allegri, Gabriella; De La Cruz, Marcia; Barrabin, Juliana; Puglisi, Celia María; Lopez, Eduardo Emilio; Lee, Hwashim; Kim, Byungioo; Delatour, Vincent; Heuillet, Maud; Nammoonnoy, Jintana; Gören, Ahmet Ceyhan; Bilsel, Gokhan; Konopelko, L.; Krylov, A.; Lopushanskaya, E.
• Año de publicación: 2018
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Metrologia, 55: 08011
• Materia: Metrología; Colesterol; Suero; Nutrición
• Institución: Instituto Nacional de Tecnología Industrial

Cholesterol is one of the most frequently measured substances in human blood/serum to assist in assessing the health status of individuals. Because of its clinical significance, CCQM-K6 Determination of Cholesterol in Serum was completed in 2000 as one of the first Key Comparison (KC) studies performed within the Organic Analysis Working Group (OAWG). The first Subsequent KC for cholesterol, CCQM-K6.1, was completed in 2001. Measurements for this second Subsequent, CCQM-K6.2, were completed in 2012. These Subsequent comparisons were conducted to enable CCQM members that had not participated in earlier studies to demonstrate their capabilities to measure a nonpolar (pKow < -2), low molecular mass (100 g/mol to 500 g/mol) metabolite in human serum at relatively high concentrations (1 mg/g to 3 mg/g) found in normal populations. Successful participation in CCQM-K6.2 demonstrated capabilities in analysis of complex biological matrices including sample preparation (extraction, derivatization), LC or GC separation, and quantification using an isotope dilution mass spectrometry approach. Normally in a subsequent KC, no Key Comparison Reference Value (KCRV) would be established and assessment of performance would be via the deviation of participants’ results to the anchor institute’s results, adjusted to account for the anchor’s performance in the original comparison versus its KCRV. Due to the very long-time period since the original key comparison, the OAWG decided that this did not represent the best approach to assess performance in what is a relatively complex measurement. Given the excellent agreement between the anchor institute’s results and robust consensus summary of the participants’ values, the Reference Value for this study was taken as the anchor institute’s result and treated as a “KCRV”. Seven of the nine participants demonstrated agreement with the reference value.
Fil: Wise, Stephen A.. National Institute of Standards and Technology (NIST); Estados Unidos
Fil: Phinney, Karen W.. National Institute of Standards and Technology (NIST); Estados Unidos
Fil: Duewer, David L.. National Institute of Standards and Technology (NIST); Estados Unidos
Fil: Sniegoski, Lorna T.. National Institute of Standards and Technology (NIST); Estados Unidos
Fil: Welch, Michael J.. National Institute of Standards and Technology (NIST); Estados Unidos
Fil: Pabello, Guiomar. Centro Nacional de Metrologia (CENAM); México
Fil: Ávila Caldero, Marco A.. Centro Nacional de Metrologia (CENAM); México
Fil: Qinde, Liu. Health Sciences Authority (HSA); Singapur
Fil: Tong Kooi, Lee. Health Sciences Authority (HSA); Singapur
Fil: Rego, Eliane. Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO); Brasil
Fil: Garrido, Bruno. Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO); Brasil
Fil: Allegri, Gabriella. Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO); Brasil
Fil: De La Cruz, Marcia. Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO); Brasil
Fil: Barrabin, Juliana. Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO); Brasil
Fil: Puglisi, Celia María. Instituto Nacional de Tecnología Industrial (INTI); Argentina
Fil: López, Eduardo Emilio. Instituto Nacional de Tecnología Industrial (INTI); Argentina
Fil: Lee, Hwashim. Korea Research Institute of Standards and Science (KRISS); República de Corea
Fil: Kim, Byungioo. Korea Research Institute of Standards and Science (KRISS); República de Corea
Fil: Delatour, Vincent. Laboratoire National de Métrologie et d’Essais (LNE); Francia
Fil: Heuillet, Maud. Laboratoire National de Métrologie et d’Essais (LNE); Francia
Fil: Nammoonnoy, Jintana. National Institute of Metrology (NIMT); Tailandia
Fil: Gören, Ahmet Ceyhan. National Metrology Institute of Turkey (TÜBİTAK UME); Turquía
Fil: Bilsel, Gokhan. National Metrology Institute of Turkey (TÜBİTAK UME); Turquía
Fil: Konopelko, L.. D.I. Mendeleyev Institute for Metrology (VNIIM); Rusia
Fil: Krylov, A.. D.I. Mendeleyev Institute for Metrology (VNIIM); Rusia
Fil: Lopushanskaya, E.. D.I. Mendeleyev Institute for Metrology (VNIIM); Rusia