Finite Difference Model Representing Cell Distribution in Monolayer

Autores
Acerbo, Esteban; Bellotti, Mariela Ines; Bonetto, Fabian
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión aceptada
Descripción
Fil: Acerbo, Esteban. Laboratorio de Cavitación y Biotecnología, Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina . Comisión Nacional de Energía Atómica (CNEA), Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina c Consejo Nacional de Investigaciones Ciencia y Técnologicas (CONICET), Buenos Aires, C1033AAJ, Argentina d Universidad Nacional de Cuyo Instituto Balseiro, San Carlos de Bariloche, R8402AGP, Argentina
Fil: Bellotti, Mariela Ines. Universidad Nacional de Río Negro. Sede Andina. Carrera de Medicina. Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Laboratorio de Cavitación y Biotecnología. Argentina
Fil: Bonetto, Fabian. Laboratorio de Cavitación y Biotecnología, Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina . Comisión Nacional de Energía Atómica (CNEA), Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina c Consejo Nacional de Investigaciones Ciencia y Tecnológicas (CONICET), Buenos Aires, C1033AAJ, Argentina d Universidad Nacional de Cuyo Instituto Balseiro, San Carlos de Bariloche, R8402AGP, Argentina
In this work we developed a finite difference algorithm to calculate the spectral impedance of any distribution of square cells over an electrode. Allowing to simulate assays evolution, cell death dynamics, different cell morphologies and any electrode shape. The spectral impedance is calculated as a function of alpha, Rb and Cm. The same parameters proposed by Giaever and Keese to analytically model a confluent culture over an infinite electrode [1]. Using the same parameters allows us to compare the simulation results of a non-confluent culture to its confluent equivalent. As seen in the Figure, a simulation resume of a culture with 75% alive – 25% dead cells emplaced randomly. The results show the system spectral impedance between the bare electrode (100% dead cells) and GK model (100% alive cells) as expected. This model is aimed to estimate the cell population above the electrode by contrasting simulations with experimental measurements. For this it is necessary to simulate different cell distributions or assay evolutions, using a range of parameter to compare before the measurement as each simulation is computationally expensive.
Materia
Ciencias Médicas y de la Salud
Algorithm
Impedance spectral
cell
Ciencias Médicas y de la Salud
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
RID-UNRN (UNRN)
Institución
Universidad Nacional de Río Negro
OAI Identificador
oai:rid.unrn.edu.ar:20.500.12049/13461
Acceder
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network_name_str RID-UNRN (UNRN)
spelling Finite Difference Model Representing Cell Distribution in MonolayerAcerbo, EstebanBellotti, Mariela InesBonetto, FabianCiencias Médicas y de la SaludAlgorithmImpedance spectralcellCiencias Médicas y de la SaludFil: Acerbo, Esteban. Laboratorio de Cavitación y Biotecnología, Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina . Comisión Nacional de Energía Atómica (CNEA), Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina c Consejo Nacional de Investigaciones Ciencia y Técnologicas (CONICET), Buenos Aires, C1033AAJ, Argentina d Universidad Nacional de Cuyo Instituto Balseiro, San Carlos de Bariloche, R8402AGP, ArgentinaFil: Bellotti, Mariela Ines. Universidad Nacional de Río Negro. Sede Andina. Carrera de Medicina. Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Laboratorio de Cavitación y Biotecnología. ArgentinaFil: Bonetto, Fabian. Laboratorio de Cavitación y Biotecnología, Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina . Comisión Nacional de Energía Atómica (CNEA), Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina c Consejo Nacional de Investigaciones Ciencia y Tecnológicas (CONICET), Buenos Aires, C1033AAJ, Argentina d Universidad Nacional de Cuyo Instituto Balseiro, San Carlos de Bariloche, R8402AGP, ArgentinaIn this work we developed a finite difference algorithm to calculate the spectral impedance of any distribution of square cells over an electrode. Allowing to simulate assays evolution, cell death dynamics, different cell morphologies and any electrode shape. The spectral impedance is calculated as a function of alpha, Rb and Cm. The same parameters proposed by Giaever and Keese to analytically model a confluent culture over an infinite electrode [1]. Using the same parameters allows us to compare the simulation results of a non-confluent culture to its confluent equivalent. As seen in the Figure, a simulation resume of a culture with 75% alive – 25% dead cells emplaced randomly. The results show the system spectral impedance between the bare electrode (100% dead cells) and GK model (100% alive cells) as expected. This model is aimed to estimate the cell population above the electrode by contrasting simulations with experimental measurements. For this it is necessary to simulate different cell distributions or assay evolutions, using a range of parameter to compare before the measurement as each simulation is computationally expensive.2025-09-17info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://rid.unrn.edu.ar/handle/20.500.12049/13461eng6th Conference on Impedance-Based Cellular Assays (IBCA), Super C, Universidad RWTH Aachen, Alemania1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/reponame:RID-UNRN (UNRN)instname:Universidad Nacional de Río Negro2025-10-23T11:17:42Zoai:rid.unrn.edu.ar:20.500.12049/13461instacron:UNRNInstitucionalhttps://rid.unrn.edu.ar/jspui/Universidad públicaNo correspondehttps://rid.unrn.edu.ar/oai/snrdrid@unrn.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:43692025-10-23 11:17:42.702RID-UNRN (UNRN) - Universidad Nacional de Río Negrofalse
dc.title.none.fl_str_mv Finite Difference Model Representing Cell Distribution in Monolayer
title Finite Difference Model Representing Cell Distribution in Monolayer
spellingShingle Finite Difference Model Representing Cell Distribution in Monolayer
Acerbo, Esteban
Ciencias Médicas y de la Salud
Algorithm
Impedance spectral
cell
Ciencias Médicas y de la Salud
title_short Finite Difference Model Representing Cell Distribution in Monolayer
title_full Finite Difference Model Representing Cell Distribution in Monolayer
title_fullStr Finite Difference Model Representing Cell Distribution in Monolayer
title_full_unstemmed Finite Difference Model Representing Cell Distribution in Monolayer
title_sort Finite Difference Model Representing Cell Distribution in Monolayer
dc.creator.none.fl_str_mv Acerbo, Esteban
Bellotti, Mariela Ines
Bonetto, Fabian
author Acerbo, Esteban
author_facet Acerbo, Esteban
Bellotti, Mariela Ines
Bonetto, Fabian
author_role author
author2 Bellotti, Mariela Ines
Bonetto, Fabian
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Médicas y de la Salud
Algorithm
Impedance spectral
cell
Ciencias Médicas y de la Salud
topic Ciencias Médicas y de la Salud
Algorithm
Impedance spectral
cell
Ciencias Médicas y de la Salud
dc.description.none.fl_txt_mv Fil: Acerbo, Esteban. Laboratorio de Cavitación y Biotecnología, Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina . Comisión Nacional de Energía Atómica (CNEA), Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina c Consejo Nacional de Investigaciones Ciencia y Técnologicas (CONICET), Buenos Aires, C1033AAJ, Argentina d Universidad Nacional de Cuyo Instituto Balseiro, San Carlos de Bariloche, R8402AGP, Argentina
Fil: Bellotti, Mariela Ines. Universidad Nacional de Río Negro. Sede Andina. Carrera de Medicina. Argentina. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche. Laboratorio de Cavitación y Biotecnología. Argentina
Fil: Bonetto, Fabian. Laboratorio de Cavitación y Biotecnología, Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina . Comisión Nacional de Energía Atómica (CNEA), Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina c Consejo Nacional de Investigaciones Ciencia y Tecnológicas (CONICET), Buenos Aires, C1033AAJ, Argentina d Universidad Nacional de Cuyo Instituto Balseiro, San Carlos de Bariloche, R8402AGP, Argentina
In this work we developed a finite difference algorithm to calculate the spectral impedance of any distribution of square cells over an electrode. Allowing to simulate assays evolution, cell death dynamics, different cell morphologies and any electrode shape. The spectral impedance is calculated as a function of alpha, Rb and Cm. The same parameters proposed by Giaever and Keese to analytically model a confluent culture over an infinite electrode [1]. Using the same parameters allows us to compare the simulation results of a non-confluent culture to its confluent equivalent. As seen in the Figure, a simulation resume of a culture with 75% alive – 25% dead cells emplaced randomly. The results show the system spectral impedance between the bare electrode (100% dead cells) and GK model (100% alive cells) as expected. This model is aimed to estimate the cell population above the electrode by contrasting simulations with experimental measurements. For this it is necessary to simulate different cell distributions or assay evolutions, using a range of parameter to compare before the measurement as each simulation is computationally expensive.
description Fil: Acerbo, Esteban. Laboratorio de Cavitación y Biotecnología, Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina . Comisión Nacional de Energía Atómica (CNEA), Centro Atómico Bariloche, San Carlos de Bariloche, R8402AGP, Argentina c Consejo Nacional de Investigaciones Ciencia y Técnologicas (CONICET), Buenos Aires, C1033AAJ, Argentina d Universidad Nacional de Cuyo Instituto Balseiro, San Carlos de Bariloche, R8402AGP, Argentina
publishDate 2025
dc.date.none.fl_str_mv 2025-09-17
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://rid.unrn.edu.ar/handle/20.500.12049/13461
url http://rid.unrn.edu.ar/handle/20.500.12049/13461
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 6th Conference on Impedance-Based Cellular Assays (IBCA), Super C, Universidad RWTH Aachen, Alemania1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RID-UNRN (UNRN)
instname:Universidad Nacional de Río Negro
reponame_str RID-UNRN (UNRN)
collection RID-UNRN (UNRN)
instname_str Universidad Nacional de Río Negro
repository.name.fl_str_mv RID-UNRN (UNRN) - Universidad Nacional de Río Negro
repository.mail.fl_str_mv rid@unrn.edu.ar
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score 12.982451

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